Anti-N WASP 抗体 (ab187527)
Key features and details
- Rabbit polyclonal to N WASP
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-N WASP antibody
N WASP 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to N WASP -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Recombinant fragment corresponding to Human N WASP (N terminal). N-terminal 250AA
Database link: O00401 -
ポジティブ･コントロール
- OVCAR3, BT474, SW480 PC3 and MCF7 cell extracts; mouse brain and kidney extracts.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab187527の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
1/50 - 1/200.
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| WB | (1) |
1/500 - 1/2000. Predicted molecular weight: 55 kDa.
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| 特記事項 |
|---|
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IHC-P
1/50 - 1/200. |
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WB
1/500 - 1/2000. Predicted molecular weight: 55 kDa. |
ターゲット情報
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機能
Regulates actin polymerization by stimulating the actin-nucleating activity of the Arp2/3 complex. Binds to HSF1/HSTF1 and forms a complex on heat shock promoter elements (HSE) that negatively regulates HSP90 expression. -
配列類似性
Contains 1 CRIB domain.
Contains 1 WH1 domain.
Contains 2 WH2 domains. -
細胞内局在
Cytoplasm > cytoskeleton. Nucleus. Preferentially localized in the cytoplasm when phosphorylated and in the nucleus when unphosphorylated. - Information by UniProt
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参照データベース
- Entrez Gene: 8976 Human
- Entrez Gene: 73178 Mouse
- Entrez Gene: 682507 Rat
- Omim: 605056 Human
- SwissProt: O00401 Human
- SwissProt: Q91YD9 Mouse
- SwissProt: O08816 Rat
- Unigene: 143728 Human
see all -
別名
- DKFZp779G0847 antibody
- MGC48327 antibody
- N-WASP antibody
see all
画像
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Western blot - Anti-N WASP antibody (ab187527)All lanes : Anti-N WASP antibody (ab187527) at 1/3000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : U-87MG cell lysate
Lane 3 : A549 cell lysate
Lane 4 : Mouse brain tissue lysate
Lane 5 : Mouse lung tissue lysate
Lane 6 : Rat brain tissue lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP goat anti-rabbit IgG (H+L) at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 55 kDa
Exposure time: 90 secondsBlocking buffer: 3% nonfat dry milk in TBST.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N WASP antibody (ab187527)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat brain tissue labelling N WASP with ab187527 at 1/100. Magnification: 40x.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N WASP antibody (ab187527)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling N WASP with ab187527 at 1/100. Magnification: 40x.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N WASP antibody (ab187527)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse heart tissue labelling N WASP with ab187527 at 1/100. Magnification: 40x.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab187527 は 2 報の論文で使用されています。
- Hunziker A et al. Phosphoproteomic profiling of influenza virus entry reveals infection-triggered filopodia induction counteracted by dynamic cortactin phosphorylation. Cell Rep 38:110306 (2022). PubMed: 35081340
- Leduc C & Etienne-Manneville S Regulation of microtubule-associated motors drives intermediate filament network polarization. J Cell Biol 216:1689-1703 (2017). PubMed: 28432079