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リコンビナントRabMAb

Anti-N-ethylmaleimide-sensitive fusion protein 抗体 [EPR25190-1] - BSA and Azide free (ab302789)

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Western blot - Anti-N-ethylmaleimide-sensitive fusion protein antibody [EPR25190-1] (BSA and Azide free) (AB302789)
  • Western blot - Anti-N-ethylmaleimide-sensitive fusion protein antibody [EPR25190-1] (BSA and Azide free) (AB302789)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N-ethylmaleimide-sensitive fusion protein antibody [EPR25190-1] (BSA and Azide free) (AB302789)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N-ethylmaleimide-sensitive fusion protein antibody [EPR25190-1] (BSA and Azide free) (AB302789)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N-ethylmaleimide-sensitive fusion protein antibody [EPR25190-1] (BSA and Azide free) (AB302789)
  • Anti-N-ethylmaleimide-sensitive fusion protein antibody [EPR25190-1] (BSA and Azide free) (AB302789)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR25190-1] to N-ethylmaleimide-sensitive fusion protein - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat

Conjugates logo Related conjugates and formulations

Unconjugated

製品の概要

  • 製品名

    Anti-N-ethylmaleimide-sensitive fusion protein antibody [EPR25190-1] - BSA and Azide free
    N-ethylmaleimide-sensitive fusion protein 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR25190-1] to N-ethylmaleimide-sensitive fusion protein - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-Pmore details
    適用なし: ICC/IF,IHC-Fr or IP

  • 種交差性

    交差種: Mouse, Rat
    非交差種: Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Rat and mouse brain and hippocampus tissue lysate. IHC-P: Rat cerebrum and mouse hippocampus tissue.

  • 特記事項

    AB302789 is a carrier free version of AB302788. 

    AB302788 does not react in: WB and IHC-P with human samples; IHC-Fr and ICC/IF with mouse and rat tissues; IP with human and mouse samples. 

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab302789の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration. Detects a band of approximately 80 kDa (predicted molecular weight: 83 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
特記事項
WB
Use at an assay dependent concentration. Detects a band of approximately 80 kDa (predicted molecular weight: 83 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
追加情報
Is unsuitable for ICC/IF,IHC-Fr or IP.

ターゲット情報

  • 機能

    Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seem to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin.

  • 配列類似性

    Belongs to the AAA ATPase family.

  • 細胞内局在

    Cytoplasm.
  • Information by UniProt

  • 参照データベース

    • 別名

      • N ethylmaleimide sensitive factor antibody
      • N ethylmaleimide sensitive factor like protein antibody
      • N ethylmaleimide sensitive fusion protein antibody
      • N-ethylmaleimide-sensitive fusion protein antibody
      • NEM sensitive fusion protein antibody
      • NEM-sensitive fusion protein antibody
      • NSF antibody
      • NSF_HUMAN antibody
      • SKD 2 antibody
      • SKD2 antibody
      • Vesicle fusing ATPase antibody
      • Vesicle-fusing ATPase antibody
      • Vesicular fusion protein NSF antibody
      • Vesicular-fusion protein NSF antibody
      see all

    画像

    • Western blot - Anti-N-ethylmaleimide-sensitive fusion protein antibody [EPR25190-1] (BSA and Azide free) (AB302789)
      Western blot - Anti-N-ethylmaleimide-sensitive fusion protein antibody [EPR25190-1] (BSA and Azide free) (AB302789)
      All lanes : Anti-N-ethylmaleimide-sensitive fusion protein antibody [EPR25190-1] (ab302788) at 1/1000 dilution

      Lane 1 : Rat brain tissue lysate
      Lane 2 : Rat hippocampus tissue lysate
      Lane 3 : Rat heart tissue lysate
      Lane 4 : Rat lung tissue lysate
      Lane 5 : Rat kidney tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Developed using the ECL technique.

      Predicted band size: 83 kDa
      Observed band size: 80 kDa why is the actual band size different from the predicted?


      Exposure time: 180 seconds


      This data was developed using ab302788, the same antibody clone in a different buffer formulation.

      Blocking and dilution buffer: 5% NFDM/TBST

      Negative control: heart, lung, kidney (PMID: 8013662).

      This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

    • Western blot - Anti-N-ethylmaleimide-sensitive fusion protein antibody [EPR25190-1] (BSA and Azide free) (AB302789)
      Western blot - Anti-N-ethylmaleimide-sensitive fusion protein antibody [EPR25190-1] (BSA and Azide free) (AB302789)
      All lanes : Anti-N-ethylmaleimide-sensitive fusion protein antibody [EPR25190-1] (ab302788) at 1/1000 dilution

      Lane 1 : Mouse brain tissue lysate
      Lane 2 : Mouse hippocampus tissue lysate
      Lane 3 : Mouse heart tissue lysate
      Lane 4 : Mouse lung tissue lysate
      Lane 5 : Mouse kidney tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Developed using the ECL technique.

      Predicted band size: 83 kDa
      Observed band size: 80 kDa why is the actual band size different from the predicted?


      Exposure time: 180 seconds


      This data was developed using ab302788, the same antibody clone in a different buffer formulation.

      Blocking and dilution buffer: 5% NFDM/TBST

      Negative control: heart, lung, kidney (PMID: 8013662).

      This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N-ethylmaleimide-sensitive fusion protein antibody [EPR25190-1] (BSA and Azide free) (AB302789)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N-ethylmaleimide-sensitive fusion protein antibody [EPR25190-1] (BSA and Azide free) (AB302789)

      This data was developed using ab302788, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling N-ethylmaleimide-sensitive fusion protein with ab302788 at 1/500 dilution (0.944 µg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Cytoplasmic staining on rat cerebrum. The section was incubated with ab302788 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: A ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).A

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N-ethylmaleimide-sensitive fusion protein antibody [EPR25190-1] (BSA and Azide free) (AB302789)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N-ethylmaleimide-sensitive fusion protein antibody [EPR25190-1] (BSA and Azide free) (AB302789)

      This data was developed using ab302788, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue labeling N-ethylmaleimide-sensitive fusion protein with ab302788 at 1/500 dilution (0.944 µg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Cytoplasmic staining on mouse hippocampus. The section was incubated with ab302788 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: A ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N-ethylmaleimide-sensitive fusion protein antibody [EPR25190-1] (BSA and Azide free) (AB302789)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N-ethylmaleimide-sensitive fusion protein antibody [EPR25190-1] (BSA and Azide free) (AB302789)

      This data was developed using ab302788, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded rat lung tissue labeling N-ethylmaleimide-sensitive fusion protein with ab302788 at 1/500 dilution (0.944 µg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). The section was incubated with ab302788 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Negative control: No staining on rat lung is observed.

      Secondary antibody only control: A ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    • Anti-N-ethylmaleimide-sensitive fusion protein antibody [EPR25190-1] (BSA and Azide free) (AB302789)
      Anti-N-ethylmaleimide-sensitive fusion protein antibody [EPR25190-1] (BSA and Azide free) (AB302789)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab302789 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab302789 は論文での使用が確認できていません。

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