Anti-N-epsilon acetyl Lysine 抗体 [11A1] (ab409)
Key features and details
- Mouse monoclonal [11A1] to N-epsilon acetyl Lysine
- Suitable for: WB, ELISA, IHC-P
- Reacts with: Species independent
- Isotype: IgG1
製品の概要
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製品名
Anti-N-epsilon acetyl Lysine antibody [11A1] -
製品の詳細
Mouse monoclonal [11A1] to N-epsilon acetyl Lysine -
由来種
Mouse -
アプリケーション
適用あり: WB, ELISA, IHC-Pmore details -
種交差性
交差種: Species independent -
特記事項
The ability of this antibody to fix complement is unknown.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Constituents: 0.75% Glycine, 1.21% Tris, 2% Sucrose -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
11A1 -
ミエローマ
unknown -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab409の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/5000.
|
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ELISA |
1/5000 - 1/50000.
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IHC-P |
1/500 - 1/5000.
|
特記事項 |
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WB
1/500 - 1/5000. |
ELISA
1/5000 - 1/50000. |
IHC-P
1/500 - 1/5000. |
画像
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Western blot - Anti-N-epsilon acetyl Lysine antibody [11A1] (ab409)Image from Pehar M et al., J Biol Chem. 2012 Jun 29;287(27):22436-40. Epub 2012 May 24.; Fig 2.; doi: 10.1074/jbc.C112.362871; June 29, 2012, The Journal of Biological Chemistry, 287, 22436-22440.Western Blot analysis showing ab409 staining N-epsilon acetyl Lysine on immunoprecipitated ER-resident proteins from control (non-transfected) H4 cells.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (9)
ab409 は 9 報の論文で使用されています。
- Rigby MJ et al. The endoplasmic reticulum acetyltransferases ATase1/NAT8B and ATase2/NAT8 are differentially regulated to adjust engagement of the secretory pathway. J Neurochem 154:404-423 (2020). PubMed: 31945187
- Peng Y et al. Deficient import of acetyl-CoA into the ER lumen causes neurodegeneration and propensity to infections, inflammation, and cancer. J Neurosci 34:6772-89 (2014). PubMed: 24828632
- Ding Y et al. Biochemical Inhibition of the Acetyltransferases ATase1 and ATase2 Reduces ß-Secretase (BACE1) Levels and Aß Generation. J Biol Chem 287:8424-33 (2012). WB . PubMed: 22267734
- Pehar M et al. Proteomic assessment shows that many ER-resident proteins are targeted by N{varepsilon}-lysine acetylation in the lumen of the organelle and predicts broad biological impact. J Biol Chem : (2012). WB . PubMed: 22628546
- Guo B et al. Dynamic modification of the ETS transcription factor PEA3 by sumoylation and p300-mediated acetylation. Nucleic Acids Res 39:6403-13 (2011). WB . PubMed: 21543453
- Choi JK et al. Acetylation of Rsc4p by Gcn5p is essential in the absence of histone H3 acetylation. Mol Cell Biol 28:6967-72 (2008). WB . PubMed: 18809572
- Costantini C et al. A reversible form of lysine acetylation in the ER and Golgi lumen controls the molecular stabilization of BACE1. Biochem J 407:383-95 (2007). WB . PubMed: 17425515
- Mantovani F et al. The prolyl isomerase Pin1 orchestrates p53 acetylation and dissociation from the apoptosis inhibitor iASPP. Nat Struct Mol Biol 14:912-20 (2007). PubMed: 17906639
- Mantovani F et al. Pin1 links the activities of c-Abl and p300 in regulating p73 function. Mol Cell 14:625-36 (2004). PubMed: 15175157