JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB409

Anti-N-epsilon acetyl Lysine 抗体 [11A1]

Anti-N-epsilon acetyl Lysine antibody [11A1]

Be the first to review this product! Submit a review

|

(9 Publications)

Mouse Monoclonal N-epsilon acetyl Lysine antibody. Suitable for ELISA, WB, IHC-P and reacts with Modified Amino Acid samples. Cited in 9 publications.
1 Images
Western blot - Anti-N-epsilon acetyl Lysine antibody [11A1] (AB409)
  • WB

PubMed

Western blot - Anti-N-epsilon acetyl Lysine antibody [11A1] (AB409)

Western Blot analysis showing ab409 staining N-epsilon acetyl Lysine on immunoprecipitated ER-resident proteins from control (non-transfected) H4 cells.

All lanes:

Western blot - Anti-N-epsilon acetyl Lysine antibody [11A1] (ab409)

false

Image from Pehar M et al., J Biol Chem. 2012 Jun 29;287(27):22436-40. Epub 2012 May 24.; Fig 2.; doi: 10.1074/jbc.C112.362871; June 29, 2012, The Journal of Biological Chemistry, 287, 22436-22440.

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

11A1

アイソタイプ

IgG1

軽鎖のタイプ

kappa

キャリアフリー

No

アプリケーション

IHC-P, ELISA, WB

applications

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Modified Amino Acid": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "1/5000 - 1/50000", "ELISA-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/500 - 1/5000", "IHCP-species-notes": "<p></p>" } } }
style
left-column

製品の詳細

The ability of this antibody to fix complement is unknown.
style
left-column

出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
Constituents: 2% Sucrose, 1.21% Tris, 0.75% Glycine
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
style
left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

N-epsilon acetyl Lysine often known as acetylated lysine is a modified amino acid resulting from the acetylation of the lysine residue. This modification typically occurs as part of protein post-translational modifications which influence protein function and interaction. N-epsilon acetyl Lysine itself is not a standalone protein with a specific mass; rather it fits into larger proteins as an acetylation site. These acetylation events frequently take place in the nucleus primarily affecting histone proteins within chromatin structures impacting gene expression regulation.
Biological function summary

Acetylation of lysine residues plays an important role in regulating gene expression and chromatin dynamics. Acetylation neutralizes the positive charge of lysine affecting histone's binding affinity for DNA and other regulatory proteins. This modification typically leads to chromatin relaxation facilitating access to transcription factors and promoting gene transcription. N-epsilon acetyl Lysine often appears within the context of larger multiprotein complexes like the histone acetyltransferase complex which includes other proteins such as CBP/p300.

Pathways

N-epsilon acetyl Lysine is critical in chromatin remodeling and transcriptional regulation pathways. Acetylation interacts significantly with the transcriptional coactivators p300/CBP which transfer acetyl groups to specific lysines on histone tails directly impacting gene transcription. Additionally it is involved in the regulation of the cell cycle through pathways that include the p21 protein which influences cellular proliferation and differentiation.

Acetylation of lysine residues is implicated in cancer and neurological disorders. In cancer altered acetylation patterns result in aberrant gene expression as seen in proteins interacting with p53 an important tumor suppressor gene. Similarly in neurological disorders like Alzheimer's disease dysregulated lysine acetylation affects the proteins tau and amyloid precursor protein (APP) contributing to disease pathology. Understanding the role of N-epsilon acetyl Lysine within these contexts aids in pioneering targeted therapeutic strategies.
style
left-column
style
left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

ターゲットの情報

See full target information N-epsilon acetyl Lysine
style
left-column

文献 (9)

Recent publications for all applications. Explore the full list and refine your search

Journal of neurochemistry 154:404-423 PubMed31945187

2020

The endoplasmic reticulum acetyltransferases ATase1/NAT8B and ATase2/NAT8 are differentially regulated to adjust engagement of the secretory pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Michael J Rigby,Yun Ding,Mark A Farrugia,Michael Feig,Giuseppe P Cortese,Heather Mitchell,Corinna Burger,Luigi Puglielli

The Journal of neuroscience : the official journal of the Society for Neuroscience 34:6772-89 PubMed24828632

2014

Deficient import of acetyl-CoA into the ER lumen causes neurodegeneration and propensity to infections, inflammation, and cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yajing Peng,Mi Li,Ben D Clarkson,Mariana Pehar,Patrick J Lao,Ansel T Hillmer,Todd E Barnhart,Bradley T Christian,Heather A Mitchell,Barbara B Bendlin,Matyas Sandor,Luigi Puglielli

The Journal of biological chemistry 287:22436-40 PubMed22628546

2012

Proteomic assessment shows that many endoplasmic reticulum (ER)-resident proteins are targeted by N(epsilon)-lysine acetylation in the lumen of the organelle and predicts broad biological impact.

Applications

WB

Species

Unspecified reactive species

Mariana Pehar,Massimiliano Lehnus,Anna Karst,Luigi Puglielli

The Journal of biological chemistry 287:8424-33 PubMed22267734

2012

Biochemical inhibition of the acetyltransferases ATase1 and ATase2 reduces β-secretase (BACE1) levels and Aβ generation.

Applications

WB

Species

Unspecified reactive species

Yun Ding,Mi Hee Ko,Mariana Pehar,Frank Kotch,Noel R Peters,Yun Luo,Shahriar M Salamat,Luigi Puglielli

Nucleic acids research 39:6403-13 PubMed21543453

2011

Dynamic modification of the ETS transcription factor PEA3 by sumoylation and p300-mediated acetylation.

Applications

WB

Species

Unspecified reactive species

Baoqiang Guo,Niki Panagiotaki,Stacey Warwood,Andrew D Sharrocks

Molecular and cellular biology 28:6967-72 PubMed18809572

2008

Acetylation of Rsc4p by Gcn5p is essential in the absence of histone H3 acetylation.

Applications

WB

Species

Unspecified reactive species

Jennifer K Choi,Daniel E Grimes,Keegan M Rowe,Leann J Howe

Nature structural & molecular biology 14:912-20 PubMed17906639

2007

The prolyl isomerase Pin1 orchestrates p53 acetylation and dissociation from the apoptosis inhibitor iASPP.

Applications

Unspecified application

Species

Unspecified reactive species

Fiamma Mantovani,Francesca Tocco,Javier Girardini,Paul Smith,Milena Gasco,Xin Lu,Tim Crook,Giannino Del Sal

The Biochemical journal 407:383-95 PubMed17425515

2007

A reversible form of lysine acetylation in the ER and Golgi lumen controls the molecular stabilization of BACE1.

Applications

WB

Species

Unspecified reactive species

Claudio Costantini,Mi Hee Ko,Mary Cabell Jonas,Luigi Puglielli

Molecular cell 14:625-36 PubMed15175157

2004

Pin1 links the activities of c-Abl and p300 in regulating p73 function.

Applications

Unspecified application

Species

Unspecified reactive species

Fiamma Mantovani,Silvano Piazza,Monica Gostissa,Sabrina Strano,Paola Zacchi,Roberto Mantovani,Giovanni Blandino,Giannino Del Sal
View all publications
style
left-column
style
left-column
style
right-column

組合せ製品

Proteins & Peptides

AB88533

Native Human Von Willebrand Factor protein

SDS-PAGE - Native Human Von Willebrand Factor protein (AB88533)

  • 0
  • 0
Primary Antibodies

AB190479

Anti-acetyl Lysine antibody [RM101]

Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-acetyl Lysine antibody [RM101] (AB190479)

  • 5
  • 2
Primary Antibodies

AB61384

Anti-acetyl Lysine antibody [7F8]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-acetyl Lysine antibody [7F8] (AB61384)

  • 5
  • 1
Primary Antibodies

AB80178

Anti-acetyl Lysine antibody

Immunocytochemistry/ Immunofluorescence - Anti-acetyl Lysine antibody (AB80178)

  • 4
  • 9

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com