Anti-N Cadherin (phospho Y820) 抗体 (ab76059)
Key features and details
- Rabbit polyclonal to N Cadherin (phospho Y820)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-N Cadherin (phospho Y820) antibody
N Cadherin 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to N Cadherin (phospho Y820) -
由来種
Rabbit -
特異性
ab76059 detects a 130 kDa band corresponding to N cadherin in western blots of serum-starved human endothelial cells treated with pervanadate, but it is not detected in untreated cells. -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Synthetic peptide corresponding to Human N Cadherin (phospho Y820).
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ポジティブ･コントロール
- Human endothelial cells treated with pervanadate (1 mM) for 30 min.
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特記事項
Do not aliquot this antibody.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 50% Glycerol, PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab76059 was cross-adsorbed to phospho-N Cadherin (Tyr-860) and unphosphorylated N cadherin (Tyr-820) peptides before affinity purification using phospho-N cadherin (Tyr-820) peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab76059の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000. Predicted molecular weight: 100 kDa.
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| 特記事項 |
|---|
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WB
1/1000. Predicted molecular weight: 100 kDa. |
ターゲット情報
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機能
Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density. -
配列類似性
Contains 5 cadherin domains. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 1000 Human
- Entrez Gene: 12558 Mouse
- Entrez Gene: 83501 Rat
- Omim: 114020 Human
- SwissProt: P19022 Human
- SwissProt: P15116 Mouse
- SwissProt: Q9Z1Y3 Rat
- Unigene: 464829 Human
see all -
別名
- CADH2_HUMAN antibody
- Cadherin 2 antibody
- Cadherin 2 N cadherin neuronal antibody
see all
画像
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Western blot - Anti-N Cadherin (phospho Y820) antibody (ab76059)All lanes : Anti-N Cadherin (phospho Y820) antibody (ab76059) at 1/1000 dilution
Lane 1 : Human endothelial cells untreated
Lane 2 : Human endothelial cells treated with pervanadate (1 mM) for 30 min
Lane 3 : Human endothelial cells treated with pervanadate (1 mM) for 30 min with phospho-N-cadherin (Tyr-820) peptide
Lane 4 : Human endothelial cells treated with pervanadate (1 mM) for 30 min with phospho-N-cadherin (Tyr-860) peptide
Predicted band size: 100 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted?
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab76059 は 3 報の論文で使用されています。
- Zhang J et al. Identification and validation of an eight-lncRNA signature that predicts prognosis in patients with esophageal squamous cell carcinoma. Cell Mol Biol Lett 27:39 (2022). PubMed: 35578166
- Li Y et al. Apogossypolone Inhibits Cell Proliferation and Epithelial-Mesenchymal Transition in Cervical Cancer via Activating DKK3. Front Oncol 12:948023 (2022). PubMed: 35924156
- Chen C et al. Long non-coding RNA TMCC1-AS1 predicts poor prognosis and accelerates epithelial-mesenchymal transition in liver cancer. Oncol Lett 22:773 (2021). PubMed: 34589152