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Signal Transduction Cytoskeleton / ECM Cell Adhesion Cadherins
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遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-N Cadherin 抗体 [EPR1791-4] (ab76011)

  • Datasheet
  • SDS
Reviews (13)Q&A (7)References (379)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [EPR1791-4] (ab76011)
  • Western blot - Anti-N Cadherin antibody [EPR1791-4] (ab76011)
  • Western blot - Anti-N Cadherin antibody [EPR1791-4] (ab76011)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [EPR1791-4] (ab76011)
  • Western blot - Anti-N Cadherin antibody [EPR1791-4] (ab76011)
  • Western blot - Anti-N Cadherin antibody [EPR1791-4] (ab76011)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [EPR1791-4] (ab76011)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [EPR1791-4] (ab76011)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [EPR1791-4] (ab76011)
  • Anti-N Cadherin antibody [EPR1791-4] (ab76011)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1791-4] to N Cadherin
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 750 Carrier Free

こちらの製品もご検討ください

二次抗体
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
ELISA
Product image
Human IDO ELISA Kit (ab245710)
ノックアウト
Product image
Human CDH2 (N Cadherin) knockout HEK-293T cell line (ab255377)

関連製品

製品の概要

  • 製品名

    Anti-N Cadherin antibody [EPR1791-4]
    N Cadherin 一次抗体 製品一覧
  • 製品の詳細

    Rabbit monoclonal [EPR1791-4] to N Cadherin
  • 由来種

    Rabbit
  • 特異性

    The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

  • アプリケーション

    適用あり: WB, IHC-Pmore details
    適用なし: Flow Cyt or ICC/IF
  • 種交差性

    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ・コントロール

    • WB: HEK-293T, A549, PC-3, HepG2, C6, Human brain, Mouse brain, and Rat brain lysates; IHC-P: Human liver, and Human cardiac muscle tissues;
  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • バッファー

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • 精製度

    Protein A purified
  • ポリ/モノ

    モノクローナル
  • クローン名

    EPR1791-4
  • アイソタイプ

    IgG
  • 研究分野

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Cadherins
    • Stem Cells
    • Hematopoietic Progenitors
    • Surface Molecules
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Cell adhesion
    • Cadherins

関連製品

  • Alternative Versions

    • Alexa Fluor® 647 Anti-N Cadherin antibody [EPR1791-4] (ab195186)
    • Anti-N Cadherin antibody [EPR1791-4] - Low endotoxin, Azide free (ab202030)
    • Anti-N Cadherin antibody [EPR1791-4] - BSA and Azide free (ab271856)
    • Alexa Fluor® 568 Anti-N Cadherin antibody [EPR1791-4] (ab313119)
    • Alexa Fluor® 750 Anti-N Cadherin antibody [EPR1791-4] (ab321710)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human CDH2 (N Cadherin) knockout HEK-293T cell line (ab255377)
  • KO cell lysates

    • Human CDH2 (N Cadherin) knockout HEK-293T cell lysate (ab263843)
  • Positive Controls

    • HeLa membrane extract lysate (ab29547)
  • Related Products

    • Prestained Protein Ladder - Broad molecular weight (10 - 245 kDa) (ab116028)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab76011の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (3)
1/5000 - 1/20000. Predicted molecular weight: 100 kDa.
IHC-P (3)
Use at an assay dependent concentration.
特記事項
WB
1/5000 - 1/20000. Predicted molecular weight: 100 kDa.
IHC-P
Use at an assay dependent concentration.
追加情報
Is unsuitable for Flow Cyt or ICC/IF.

ターゲット情報

  • 機能

    Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density.
  • 配列類似性

    Contains 5 cadherin domains.
  • 細胞内局在

    Cell membrane.
  • Target information above from: UniProt accession P19022 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 1000 Human
    • Entrez Gene: 12558 Mouse
    • Entrez Gene: 83501 Rat
    • Omim: 114020 Human
    • SwissProt: P19022 Human
    • SwissProt: P15116 Mouse
    • SwissProt: Q9Z1Y3 Rat
    • Unigene: 464829 Human
    • Unigene: 606106 Human
    • Unigene: 257437 Mouse
    • Unigene: 23200 Rat
    see all
  • 別名

    • CADH2_HUMAN antibody
    • Cadherin 2 antibody
    • Cadherin 2 N cadherin neuronal antibody
    • Cadherin 2 type 1 antibody
    • Cadherin 2 type 1 N cadherin neuronal antibody
    • Cadherin 2, type 1, N-cadherin (neuronal) antibody
    • Cadherin-2 antibody
    • Cadherin2 antibody
    • Calcium dependent adhesion protein neuronal antibody
    • CD325 antibody
    • CD325 antigen antibody
    • CDH2 antibody
    • CDHN antibody
    • CDw325 antibody
    • CDw325 antigen antibody
    • N cadherin 1 antibody
    • N-cadherin antibody
    • NCAD antibody
    • Neural cadherin antibody
    • OTTHUMP00000066304 antibody
    • OTTHUMP00000067378 antibody
    see all

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [EPR1791-4] (ab76011)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [EPR1791-4] (ab76011)

    Immunohistochemical analysis of formalin-fixed paraffin-embedded human heart labelling N Cadherin with ab271856 at a concentration of 1µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with an OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was conducted for 32min with ULTRA cell conditioning solution (CC1 pH8.5). ab271856 anti N Cadherin antibody was incubated at 37°C for 16min. Sections were counterstained is with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.

    This data was developed using the same antibody clone in a different buffer formulation (ab271856).

  • Western blot - Anti-N Cadherin antibody [EPR1791-4] (ab76011)
    Western blot - Anti-N Cadherin antibody [EPR1791-4] (ab76011)
    All lanes : Anti-N Cadherin antibody [EPR1791-4] (ab76011) at 1/5000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : cdh2 knockout HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

    Performed under reducing conditions.

    Predicted band size: 100 kDa
    Observed band size: 125 kDa why is the actual band size different from the predicted?



    False colour image of Western blot: Anti-N Cadherin antibody [EPR1791-4] staining at 1/5000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab76011 was shown to bind specifically to N Cadherin. A band was observed at 125 kDa in wild-type HeLa cell lysates with no signal observed at this size in cdh2 knockout cell line ab274934 (knockout cell lysate ab274992).

     

    To generate this image, wild-type and cdh2 knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

  • Western blot - Anti-N Cadherin antibody [EPR1791-4] (ab76011)
    Western blot - Anti-N Cadherin antibody [EPR1791-4] (ab76011)
    All lanes : Anti-N Cadherin antibody [EPR1791-4] (ab76011) at 1/1000 dilution

    Lane 1 : HeLa Whole Cell Lysate
    Lane 2 : HeLa Whole Cell Lysate (Scraped)
    Lane 3 : Human Brain Tissue Lysate
    Lane 4 : Mouse Brain Tissue Lysate
    Lane 5 : Rat Brain Tissue Lysate
    Lane 6 : MCF7 Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 100 kDa
    Observed band size: 125 kDa why is the actual band size different from the predicted?



    This blot was produced using a 4-12% Bis-tris under the MOPS buffer system. The gel was run at 200V for 55 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was blocked for an hour using 3% milk before ab76011 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at a 1/1000 dilution and 1/20000 dilution respectively. Antibody binding was detected using Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [EPR1791-4] (ab76011)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [EPR1791-4] (ab76011)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human cardiac muscle tissue sections labeling N Cadherin with purified ab76011 at 1:50 dilution (1.94 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Western blot - Anti-N Cadherin antibody [EPR1791-4] (ab76011)
    Western blot - Anti-N Cadherin antibody [EPR1791-4] (ab76011)
    All lanes : Anti-N Cadherin antibody [EPR1791-4] (ab76011) at 1/5000 dilution

    Lane 1 : Wild-type HEK-293T cell lysate
    Lane 2 : CDH2 knockout HEK-293T cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 100 kDa



    Lanes 1 - 2: Merged signal (red and green). Green - ab76011 observed at 125 kDa. Red - loading control, ab8245 observed at 37 kDa.  

     ab76011 was shown to react with N Cadherin in wild-type HEK-293T. Loss of signal was observed when knockout cell line ab255377 (knockout cell lysate ab263843) was used. Wild-type and N Cadherin knockout samples were subjected to SDS-PAGE. ab76011 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 5000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

     

  • Western blot - Anti-N Cadherin antibody [EPR1791-4] (ab76011)
    Western blot - Anti-N Cadherin antibody [EPR1791-4] (ab76011)
    All lanes : ab76011, Anti-N Cadherin antibody [EPR1791-4] (Left) or ab207608, Anti-N Cadherin antibody [EPR19654] (Right) at 1/1000 dilution

    Lane 1 : A549 (Human lung carcinoma epithelial cell) whole cell lysates prepared in RIPA lysis method
    Lane 2 : PC-3 (Human prostate adenocarcinoma epithelial cell) whole cell lysates prepared in RIPA lysis method
    Lane 3 : HCT 116 (Human colorectal carcinoma epithelial cell) whole cell lysates prepared in RIPA lysis method
    Lane 4 : HCT 116 (Human colorectal carcinoma epithelial cell) whole cell lysates prepared in 1%SDS Hot lysis method
    Lane 5 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate prepared in RIPA lysis method
    Lane 6 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate prepared in 1%SDS Hot lysis method
    Lane 7 : C6 (Rat glial tumor glial cell) whole cell lysates prepared in RIPA lysis method
    Lane 8 : C6 (Rat glial tumor glial cell) whole cell lysates prepared in 1%SDS Hot lysis method
    Lane 9 : Human brain lysates prepared in 1%SDS Hot lysis method
    Lane 10 : Mouse brain lysates prepared in 1%SDS Hot lysis method
    Lane 11 : Rat brain lysates prepared in 1%SDS Hot lysis method

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 100 kDa
    Observed band size: 110-130 kDa why is the actual band size different from the predicted?



    The molecular weight observed is consistent with what has been described in the literature (PMID: 22553038). This antibody fails to detect N Cadherin in HCT 116 cell which is positive described in the literature (PMID: 23431386 and 26540342)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [EPR1791-4] (ab76011)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [EPR1791-4] (ab76011)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human liver tissue sections labeling N Cadherin with purified ab76011 at 1:50 dilution (1.94 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [EPR1791-4] (ab76011)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [EPR1791-4] (ab76011)

    Immunohistochemistry of kidney carcinoma staining N Cadherin with ab76011 at 1μg/ml

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [EPR1791-4] (ab76011)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [EPR1791-4] (ab76011)This image is courtesy of an anonymous Abreview

    ab76011 staining N Cadherin in Mouse pancreas tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA + 1% FBS for 2 hours at room temperature; antigen retrieval was by heat mediation in a citrate buffer pH6. Samples were incubated with primary antibody (1/500 in 1% BSA + 1% FBS) for 16 hours at 4°C. An undiluted HRP-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

  • Anti-N Cadherin antibody [EPR1791-4] (ab76011)
    Anti-N Cadherin antibody [EPR1791-4] (ab76011)

プロトコール

  • Western blot protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

データシートおよび資料

  • SDS download

  • Datasheet download

    Download

参考文献 (379)

ab76011 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab76011 は 379 報の論文で使用されています。

  • Wang M  et al. miR-638 suppresses cervical cancer progression by inhibiting NCAPG2 under the treatment of Tetrandrine. Histol Histopathol 39:497-509 (2024). PubMed: 37702425
  • Xia YT  et al. Suppression of migration and invasion by taraxerol in the triple-negative breast cancer cell line MDA-MB-231 via the ERK/Slug axis. PLoS One 18:e0291693 (2023). PubMed: 37751436
  • Shi L  et al. Circular RNA circWHSC1 facilitates colorectal cancer cell proliferation by targeting miR-130a-5p/zeb1 signaling in vitro and in vivo. Heliyon 9:e20176 (2023). PubMed: 37810854
  • Gu W & Yang C Zinc oxide nanoparticles inhibit malignant progression and chemotherapy resistance of ovarian cancer cells by activating endoplasmic reticulum stress and promoting autophagy. Exp Ther Med 26:508 (2023). PubMed: 37840563
  • Xu X  et al. TPPP3 promote epithelial-mesenchymal transition via Snail1 in glioblastoma. Sci Rep 13:17960 (2023). PubMed: 37863960
View all Publications for this product

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

1-10 of 20 Abreviews or Q&A

Immunohistochemistry (Frozen sections) abreview for Anti-N Cadherin antibody [EPR1791-4]

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (embryonic head mesenchyme)
Specification
embryonic head mesenchyme
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Feb 10 2024

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-N Cadherin antibody [EPR1791-4]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: ER2
Permeabilization
No
Specification
liver
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. 勝啓 平田

Verified customer

投稿 Dec 15 2022

Immunocytochemistry/ Immunofluorescence abreview for Anti-N Cadherin antibody [EPR1791-4]

Average
Abreviews
Abreviews
This product is known to not work in this application or species.
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Brain)
Permeabilization
No
Specification
Brain
Blocking step
Serum as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 28°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Oct 09 2019

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-N Cadherin antibody [EPR1791-4]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Cow Tissue sections (oviductal tissues)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EDTA pH9.0
Permeabilization
No
Specification
oviductal tissues
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 2.5% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Yoshihiko Kobayashi

Verified customer

投稿 Sep 21 2016

Western blot abreview for Anti-N Cadherin antibody [EPR1791-4]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (MCF-7 cell)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
30 µg
Specification
MCF-7 cell
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Mar 11 2016

Immunohistochemistry (Frozen sections) abreview for Anti-N Cadherin antibody [EPR1791-4]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Embryonic mouse brain coronal cortical section)
Permeabilization
Yes - Triton X 100
Specification
Embryonic mouse brain coronal cortical section
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Paraformaldehyde
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The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Bhavin Shah

Verified customer

投稿 Oct 20 2015

Western blot abreview for Anti-N Cadherin antibody [EPR1791-4]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (10)
Sample
Mouse Cell lysate - whole cell (MEFs)
Specification
MEFs
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Dec 16 2013

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
1%BSA 1%FBS as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer pH 6
Sample
Mouse Tissue sections (Pancreas)
Specification
Pancreas
Permeabilization
Yes - TBS-Tween 0,1%
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Jun 06 2013

Immunocytochemistry/ Immunofluorescence abreview for Anti-N Cadherin antibody [EPR1791-4]

Excellent
Abreviews
Abreviews
This product is known to not work in this application or species.
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (MEFs wild type)
Specification
MEFs wild type
Fixative
Paraformaldehyde
Permeabilization
Yes - 0,3% Triton PBS
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Mar 18 2013

Question

Could you tell me the sequences used to raise ab76011 (EPR1791-4) and ab19348 (8C11), both of which are anti-N-Cadherin antibodies.

Thankyou

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Abcam community

Verified customer

Asked on Jul 23 2012

Answer

Thank you for contacting us and your interest in our products.
The anti-N Cadherin antibody [8C11], ab19348, was raised against a bacterial fusion protein corresponding to the extracellular domain of N-cadherin (a domain between EC3 and EC4, amino acid residues 92-593 from human N-cadherin).
The anti-N Cadherin antibody [EPR1791-4], ab76011, meanwhile was raised against a synthetic peptide taken from within the residues 100-200 of human N-cadherin , also within the extracellular domain of the protein.
I hope this information has been of help. If you have any further questions, please do not hesitate to contact us again.

Read More

Abcam Scientific Support

Answered on Jul 23 2012

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