JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB10149

Anti-MYRIP 抗体

Anti-MYRIP antibody

4

(1 Review)

|

(4 Publications)

Goat Polyclonal MYRIP antibody. Suitable for WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human MYRIP aa 800 to C-terminus.

別名を表示する

SLAC2C, MYRIP, Rab effector MyRIP, Exophilin-8, Myosin-VIIa- and Rab-interacting protein, Synaptotagmin-like protein lacking C2 domains C, SlaC2-c, Slp homolog lacking C2 domains c

1 Images
Western blot - Anti-MYRIP antibody (AB10149)
  • WB

Unknown

Western blot - Anti-MYRIP antibody (AB10149)

All lanes:

Western blot - Anti-MYRIP antibody (ab10149) at 0.3 µg/mL

All lanes:

Human Amygdala lysates at 35 µg

Predicted band size: 96 kDa

true

Key facts

宿主種

Goat

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

WB

applications

免疫原

Synthetic Peptide within Human MYRIP aa 800 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q8NFW9

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.3 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>" } } }
style
left-column

製品の詳細

A molecular complex composed of Rab27A, MYRIP and myosin VIIa bridges retinal melanosomes to the actin cytoskeleton and thereby mediates the local trafficking of these organelles. The defect of this molecular complex is likely to account for the perinuclear mislocalization of the melanosomes observed in the retinal pigment epithelium cells of myosin VIIa-defective mice.
style
left-column

出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Immunogen
精製に関する特記事項
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
バッファー組成
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
style
left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

MYRIP also known as Myosin VIIA and Rab Interacting Protein plays a role in linking Rab27A to myosin motor proteins which is essential for the transportation of cellular components. It is a protein with a molecular mass of approximately 131 kDa. MYRIP expresses in various tissues including the retina where it supports the function of photoreceptor cells. The protein also shows presence in specialized secretory cells contributing to the regulation of secretory pathways.
Biological function summary

MYRIP facilitates the connection between specific motor proteins and vesicle-bound Rab proteins forming part of a complex responsible for vesicle movement along actin filaments. By acting as an adapter it links the actin cytoskeleton with membrane-bound organelles and vesicles influencing intracellular transport and distribution. Its role in these complexes indicates its involvement in cellular trafficking mechanisms necessary for maintaining cellular organization and function.

Pathways

MYRIP engages in the exocytosis pathway and melanosome transport. It interacts with Rab27A participating in the pathway that mediates the transport of melanosomes within cells. This interaction is key to controlling the delivery of pigment granules in melanocytes. Additionally the exocytosis pathway relies on MYRIP for effective secretion where it works alongside other proteins like Rab27B to manage vesicle docking and fusion at the plasma membrane.

MYRIP relates to conditions like Griscelli syndrome type 1 which involves a defect in the transport of melanosomes. Changes or malfunctions in MYRIP or its associated proteins including Rab27A disrupt normal pigment distribution leading to this type of disorder. Furthermore given its expression in the retina MYRIP also connects to visual impairments wherein its dysfunction could affect photoreceptor cell health.
style
left-column
style
left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

ターゲットの情報

Rab effector protein involved in melanosome transport. Serves as link between melanosome-bound RAB27A and the motor proteins MYO5A and MYO7A. May link RAB27A-containing vesicles to actin filaments. Functions as a protein kinase A-anchoring protein (AKAP). May act as a scaffolding protein that links PKA to components of the exocytosis machinery, thus facilitating exocytosis, including insulin release (By similarity).
See full target information MYRIP
style
left-column

文献 (4)

Recent publications for all applications. Explore the full list and refine your search

eLife 12: PubMed36803984

2023

Functional hierarchy among different Rab27 effectors involved in secretory granule exocytosis.

Applications

Unspecified application

Species

Unspecified reactive species

Kunli Zhao,Kohichi Matsunaga,Kouichi Mizuno,Hao Wang,Katsuhide Okunishi,Tetsuro Izumi

Journal of cell science 129:592-603 PubMed26675235

2015

Interaction between MyRIP and the actin cytoskeleton regulates Weibel-Palade body trafficking and exocytosis.

Applications

Unspecified application

Species

Unspecified reactive species

Ianina L Conte,Nicola Hellen,Ruben Bierings,Gregory I Mashanov,Jean-Baptiste Manneville,Nikolai I Kiskin,Matthew J Hannah,Justin E Molloy,Tom Carter

Proceedings of the National Academy of Sciences of 106:14966-71 PubMed19706440

2009

TLR4-mediated expulsion of bacteria from infected bladder epithelial cells.

Applications

IP

Species

Human

Jeongmin Song,Brian L Bishop,Guojie Li,Richard Grady,Ann Stapleton,Soman N Abraham

Blood 113:5010-8 PubMed19270261

2009

Rab27a and MyRIP regulate the amount and multimeric state of VWF released from endothelial cells.

Applications

ICC/IF

Species

Human

Thomas D Nightingale,Krupa Pattni,Alistair N Hume,Miguel C Seabra,Daniel F Cutler
View all publications
style
left-column
style
left-column
style
right-column

組合せ製品

Primary Antibodies

AB78298

Anti-SEL1L antibody

Western blot - Anti-SEL1L antibody (AB78298)

  • 3
  • 3
Primary Antibodies

AB109363

Anti-RENT1/hUPF1 antibody [EPR4681]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-RENT1/hUPF1 antibody [EPR4681] (AB109363)

  • 5
  • 2
Primary Antibodies

AB124267

Anti-MARK4 antibody

Immunocytochemistry/ Immunofluorescence - Anti-MARK4 antibody (AB124267)

  • 0
  • 0
Primary Antibodies

AB109450

Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479]

RabMAb|Recombinant

Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) antibody [EPR5479] (AB109450)

  • 5
  • 2

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com