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Anti-Myosin Phosphatase (phospho T696) 抗体 (ab59202)

Reviews (1)Q&A (3)References (2)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin Phosphatase (phospho T696) antibody (ab59202)

    Key features and details

    • Rabbit polyclonal to Myosin Phosphatase (phospho T696)
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG

    製品の概要

    • 製品名

      Anti-Myosin Phosphatase (phospho T696) antibody
      Myosin Phosphatase 一次抗体 製品一覧

    • 製品の詳細

      Rabbit polyclonal to Myosin Phosphatase (phospho T696)

    • 由来種

      Rabbit

    • アプリケーション

      適用あり: IHC-Pmore details

    • 種交差性

      交差種: Human
      交差が予測される動物種: Mouse, Rat

    • 免疫原

      Synthetic peptide corresponding to Human Myosin Phosphatase aa 650-750 (phospho T696).
      Database link: O14974

    • ポジティブ･コントロール

      • Human breast carcinoma tissue

    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    • 製品の状態

      Liquid

    • 保存方法

      Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

    • バッファー

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

      Without Mg+2 and Ca+2
    • Concentration information loading...

    • 精製度

      Immunogen affinity purified

    • 特記事項（精製）

      Affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.

    • ポリ/モノ

      ポリクローナル

    • アイソタイプ

      IgG

    • 研究分野

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab59202の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    IHC-P
    Use at an assay dependent concentration.
    特記事項
    IHC-P
    Use at an assay dependent concentration.

    ターゲット情報

    • 機能

      Regulates myosin phosphatase activity.

    • 組織特異性

      Expressed in striated muscles, specifically in type 2a fibers (at protein level).

    • 配列類似性

      Contains 6 ANK repeats.

    • 発生段階

      Induced by 2-fold during pregnancy, including in abdominus rectus muscle.

    • ドメイン

      Heterotetramerization is mediated by the interaction between a coiled-coil of PRKG1 and the leucine/isoleucine zipper of PPP1R12A/MBS, the myosin-binding subunit of the myosin phosphatase.

    • 翻訳後修飾

      Phosphorylated by CIT (Rho-associated kinase) (By similarity). Phosphorylated cooperatively by ROCK1 and CDC42BP on Thr-696. Phosphorylated on upon DNA damage, probably by ATM or ATR.
      In vitro, phosphorylation of Ser-695 by PKA and PKG appears to prevent phosphorylation of the inhibitory site Thr-696, probably mediated by PRKG1.

    • 細胞内局在

      Cytoplasm. Along actomyosin filaments and stress fibers.
    • Information by UniProt

    • 参照データベース

      see all

    • 別名

      • M130 antibody
      • MBS antibody
      • Myosin binding subunit antibody
      • Myosin phosphatase target subunit 1 antibody
      • Myosin phosphatase targeting subunit 1 antibody
      • Myosin phosphatase-targeting subunit 1 antibody
      • MYPT1 antibody
      • MYPT1_HUMAN antibody
      • PPP1R12A antibody
      • Protein phosphatase 1 regulatory inhibitor subunit 12A antibody
      • Protein phosphatase 1 regulatory subunit 12A antibody
      • Protein phosphatase 1, regulatory (inhibitor) subunit 12A antibody
      • Protein phosphatase myosin binding subunit antibody
      • Protein phosphatase myosin-binding subunit antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin Phosphatase (phospho T696) antibody (ab59202)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin Phosphatase (phospho T696) antibody (ab59202)
      Immunohistochemical analysis of paraffin embedded human breast carcinoma tissue using ab59202 at 1/50 dilution. Samples were untreated (left) or treated with immunizing peptide (right).

    参考文献 (2)

    ab59202 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab59202 は 2 報の論文で使用されています。

    • Yin Y  et al. Expression of MYPT1, CPI-17 and MLC20 in ileum of neonatal mouse NEC model and its significance. Exp Ther Med 14:2221-2227 (2017). PubMed: 28962146
    • Sastre E  et al. Decompensated liver cirrhosis and neural regulation of mesenteric vascular tone in rats: role of sympathetic, nitrergic and sensory innervations. Sci Rep 6:31076 (2016). WB ; Rat . PubMed: 27484028

    レビューと Q&A

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    1-4 of 4 Abreviews or Q&A

    Western blot abreview for Anti-Myosin Phosphatase (phospho T696) antibody

     Inconclusive
    Abreviews
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Hela)
    Loading amount
    120 µg
    Specification
    Hela
    Treatment
    10ng/ml IL6 for 30 minutes
    Gel Running Conditions
    Reduced Denaturing (10)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Sep 02 2008

    Question

    Thanks—do you sell this polymer kit, or do you have a spec sheet for it? I have a tyramide amplification reagent, for example, and I’d like to determine if this is a similar reagent or different.

    Read More
    Answer

    Thank you for your inquiry.

    We don't have a polymer kit besides our mouse on mouse polymer kit, ab127055.

    https://www.abcam.com/Mouse-on-Mouse-MOM-Polymer-IHC-Kit-ab127055.html

    We have ABC kits and EXPOSE kits.

    https://www.abcam.com/Mouse-and-Rabbit-Specific-HRPDAB-ABC-detection-IHC-kit-ab64264.html

    https://www.abcam.com/index.html?pageconfig=resource&rid=12865

    I hope this information helps. Please contact us with any other questions.

    Read More

    Question

    Thanks very much. One more question: can you please clarify what the Polymer Enhancer is in step 18?

    Read More
    Answer

    Thank you for your reply.


    This is just to go along with the polymer kit that was used. If you are using polymer detection, simply follow the manufacturers instructions. You can omit this step if not using this type of amplification.


    I hope this information is helpful. Please do not hesitate to contact me if you have any additional questions.

    Read More

    Question

    What is the recommended IHC protocol to use with this antibody?

    Read More
    Answer

    Thank you for contacting Abcam regarding ab59202.



    The IHC protocol is as follows:



    Deparaffinization

    1. Incubate slide at 60℃ for 60 minutes.

    2. Deparaffinize in Xylene for 10 minutes and repeat one more times.

    3. Hydrate in 100% alcohol for 5 minutes, in 95% alcohol for 5 minutes, in 85% alcohol for 5 minutes, in 75% alcohol for 5 minutes.

    4. Dip into Distill Water for 5 minutes.

    5. Dip into TBS (50 mM Tris, 100 mM NaCl, pH 7.6), leave for 5 minutes, and repeat two times.



    Antigen Retrieval

    6. Bring 500 - 2000 ml 10 mM citrate buffer (pH6.0) to the boil in a stainless steel pressure cooker.

    7. Put the slide into staining rack and lower into pressure cooker ensuring the slide is well immersed in citrate buffer.

    8. When the pressure indicator valve has risen after 3-4 minutes, incubate for 1 minute.

    9. Cool the slide naturally to room temperature.

    10. Dip into distilled water, leave for 5 minutes, and repeat two times.

    11. Dip the slide in TBS for 5 minutes and repeat two times.

    12. Immerse slides in 3% H2O2 (in fresh methanol) for 15 minutes at room temperature.

    13. Wash with distilled water two times, 5 minutes each time.

    14. Wash with TBS (pH 7.6) two times, 5 minutes each time.



    Staining with Primary Antibody

    15. Dilute primary antibody with 3% BSA in TBS to 1:50. Cover the tissue section on the slide with diluted primary antibody (use 50 – 150μl for each slide).

    16. Incubate at 37℃ for 30 minutes or at room temperature for 60 minutes (The optimal incubation time, incubation temperature, and antibody dilution should be determined by the individual laboratory).

    17. Wash with TBS two times, 5 minutes each time.



    Staining with Secondary Antibody

    18. Incubate with 100-200μl Polymer Enhancer. Incubate 30 minutes at 37℃.

    19. Wash with TBS for 3 times, 5 minutes each time.

    20. Incubate with 100-200μl Polymerized HRP and incubate 30 minutes at 37℃

    21. Wash with TBS for 3 times, 5 minutes each time.

    22. Add DAB solution and incubate 3-10 minutes (The reaction progress and the optimal time should be determine according to microscope).

    23. Wash with distilled water for 2 times, 5 minutes each time.

    24. Counterstain sections in hematoxylin if required,wash with distilled water.Immerse slides in 0.1% HCl ethanol for 1-10 seconds, wash with distilled water.

    25. Dehydrate through 95% ethanol for 1 minute, 100%



    I hope this information is helpful. Please do not hesitate to contact me if you have any additional questions.

    Read More

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