Anti-Myosin Phosphatase 抗体 (ab70809)
Key features and details
- Rabbit polyclonal to Myosin Phosphatase
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Myosin Phosphatase antibody
Myosin Phosphatase 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Myosin Phosphatase -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Gorilla, Orangutan
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免疫原
Synthetic peptide corresponding to a region between residue 800 and the C-terminus (residue 842) of human Myosin Phosphatase (NP_002471.1)
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ポジティブ･コントロール
- Whole cell lysate from HeLa or 293T cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab70809の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (2) |
1/2000 - 1/10000. Detects a band of approximately 140 kDa (predicted molecular weight: 115 kDa).
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| IP | (1) |
Use at 2-5 µg/mg of lysate.
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| 特記事項 |
|---|
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WB
1/2000 - 1/10000. Detects a band of approximately 140 kDa (predicted molecular weight: 115 kDa). |
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IP
Use at 2-5 µg/mg of lysate. |
ターゲット情報
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機能
Regulates myosin phosphatase activity. -
組織特異性
Expressed in striated muscles, specifically in type 2a fibers (at protein level). -
配列類似性
Contains 6 ANK repeats. -
発生段階
Induced by 2-fold during pregnancy, including in abdominus rectus muscle. -
ドメイン
Heterotetramerization is mediated by the interaction between a coiled-coil of PRKG1 and the leucine/isoleucine zipper of PPP1R12A/MBS, the myosin-binding subunit of the myosin phosphatase. -
翻訳後修飾
Phosphorylated by CIT (Rho-associated kinase) (By similarity). Phosphorylated cooperatively by ROCK1 and CDC42BP on Thr-696. Phosphorylated on upon DNA damage, probably by ATM or ATR.
In vitro, phosphorylation of Ser-695 by PKA and PKG appears to prevent phosphorylation of the inhibitory site Thr-696, probably mediated by PRKG1. -
細胞内局在
Cytoplasm. Along actomyosin filaments and stress fibers. - Information by UniProt
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参照データベース
- Entrez Gene: 4659 Human
- Entrez Gene: 17931 Mouse
- Entrez Gene: 397190 Pig
- Entrez Gene: 116670 Rat
- Omim: 602021 Human
- SwissProt: O14974 Human
- SwissProt: Q9DBR7 Mouse
- SwissProt: Q10728 Rat
see all -
別名
- M130 antibody
- MBS antibody
- Myosin binding subunit antibody
see all
画像
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Western blot - Anti-Myosin Phosphatase antibody (ab70809)All lanes : Anti-Myosin Phosphatase antibody (ab70809) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 115 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds -
Immunoprecipitation - Anti-Myosin Phosphatase antibody (ab70809)1mg whole lysate from HeLa cells was immunoprecipitated using ab70809 at 3ug/mg of lysate (lane 1) or a control rabbit Ig (lane 2). For the subsequent western blot, 20% of the immunoprecipitate was loaded per lane, and probed with ab70809 at 1ug/ml. Detection: chemiluminescence with exposure time of 10 seconds.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab70809 は 3 報の論文で使用されています。
- Betancor G et al. MX2-mediated innate immunity against HIV-1 is regulated by serine phosphorylation. Nat Microbiol 6:1031-1042 (2021). PubMed: 34282309
- Sastre E et al. Decompensated liver cirrhosis and neural regulation of mesenteric vascular tone in rats: role of sympathetic, nitrergic and sensory innervations. Sci Rep 6:31076 (2016). WB ; Rat . PubMed: 27484028
- Schein A et al. Identification of antisense long noncoding RNAs that function as SINEUPs in human cells. Sci Rep 6:33605 (2016). PubMed: 27646849