Anti-Myosin Phosphatase 抗体 (ab59235)
Key features and details
- Rabbit polyclonal to Myosin Phosphatase
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Myosin Phosphatase antibody
Myosin Phosphatase 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Myosin Phosphatase -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic non-phosphopeptide derived from human Myosin Phosphatase around the phosphorylation site of threonine 853 (R-S-TP-G-V).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg+2 and Ca+2 -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab59235の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
Use at an assay dependent concentration.
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| WB |
1/500 - 1/1000. Detects a band of approximately 130 kDa (predicted molecular weight: 115 kDa).
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| 特記事項 |
|---|
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IHC-P
Use at an assay dependent concentration. |
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WB
1/500 - 1/1000. Detects a band of approximately 130 kDa (predicted molecular weight: 115 kDa). |
ターゲット情報
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機能
Regulates myosin phosphatase activity. -
組織特異性
Expressed in striated muscles, specifically in type 2a fibers (at protein level). -
配列類似性
Contains 6 ANK repeats. -
発生段階
Induced by 2-fold during pregnancy, including in abdominus rectus muscle. -
ドメイン
Heterotetramerization is mediated by the interaction between a coiled-coil of PRKG1 and the leucine/isoleucine zipper of PPP1R12A/MBS, the myosin-binding subunit of the myosin phosphatase. -
翻訳後修飾
Phosphorylated by CIT (Rho-associated kinase) (By similarity). Phosphorylated cooperatively by ROCK1 and CDC42BP on Thr-696. Phosphorylated on upon DNA damage, probably by ATM or ATR.
In vitro, phosphorylation of Ser-695 by PKA and PKG appears to prevent phosphorylation of the inhibitory site Thr-696, probably mediated by PRKG1. -
細胞内局在
Cytoplasm. Along actomyosin filaments and stress fibers. - Information by UniProt
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参照データベース
- Entrez Gene: 4659 Human
- Entrez Gene: 17931 Mouse
- Omim: 602021 Human
- SwissProt: O14974 Human
- SwissProt: Q9DBR7 Mouse
- Unigene: 49582 Human
- Unigene: 422959 Mouse
- Unigene: 482714 Mouse
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別名
- M130 antibody
- MBS antibody
- Myosin binding subunit antibody
see all
画像
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Western blot - Anti-Myosin Phosphatase antibody (ab59235)All lanes : Anti-Myosin Phosphatase antibody (ab59235) at 1/500 dilution
Lane 1 : NIH3T3 cell extract
Lane 2 : NIH3T3 cell extract with immunising peptide
Predicted band size: 115 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin Phosphatase antibody (ab59235)ab59235 at 1/50 dilution staining Myosin Phosphatase in human brain by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (9)
ab59235 は 9 報の論文で使用されています。
- Hanaki S et al. PP1 regulatory subunit NIPP1 regulates transcription of E2F1 target genes following DNA damage. Cancer Sci 112:2739-2752 (2021). PubMed: 33939241
- Ma L et al. Leukocyte Rho kinase activity and serum cystatin C affect cardiovascular events in acute coronary syndrome. Medicine (Baltimore) 99:e20060 (2020). PubMed: 32664054
- Jia Z et al. Offsetting Expression Profiles of Prognostic Markers in Prostate Tumor vs. Its Microenvironment. Front Oncol 9:539 (2019). PubMed: 31316912
- Wan S et al. HMGCS2 functions as a tumor suppressor and has a prognostic impact in prostate cancer. Pathol Res Pract 215:152464 (2019). PubMed: 31176575
- Liang Y et al. Decreased Expression of MYPT1 Contributes to Tumor Angiogenesis and Poor Patient Prognosis in Human Prostate Cancer. Curr Mol Med 18:100-108 (2018). PubMed: 29974831
- Wang XQ et al. Resveratrol enhances vascular reactivity in mice following lipopolysaccharide challenge via the RhoA-ROCK-MLCP pathway. Exp Ther Med 14:308-316 (2017). PubMed: 28672931
- Yin Y et al. Expression of MYPT1, CPI-17 and MLC20 in ileum of neonatal mouse NEC model and its significance. Exp Ther Med 14:2221-2227 (2017). PubMed: 28962146
- Montalvo J et al. ROCK1 & 2 perform overlapping and unique roles in angiogenesis and angiosarcoma tumor progression. Curr Mol Med 13:205-19 (2013). WB . PubMed: 22934846
- Fu K et al. Tra2ß Protein Is Required for Tissue-specific Splicing of a Smooth Muscle Myosin Phosphatase Targeting Subunit Alternative Exon. J Biol Chem 287:16575-85 (2012). WB ; Mouse . PubMed: 22437831