Anti-Myosin light chain kinase/MLCK 抗体 [EP1458Y] (ab76092)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain kinase/MLCK antibody [EP1458Y] (ab76092)](https://www.abcam.co.jp/ps/products/76/ab76092/Images/ab76092-8-antimyosin-lightchain-kinase-mlck-antibody-ep1458y-immunohistochemistry-colon-rat.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain kinase/MLCK antibody [EP1458Y] (ab76092)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1458Y] to Myosin light chain kinase/MLCK
- Suitable for: IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Myosin light chain kinase/MLCK antibody [EP1458Y]
Myosin light chain kinase/MLCK 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP1458Y] to Myosin light chain kinase/MLCK -
由来種
Rabbit -
アプリケーション
適用あり: IHC-Pmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human Myosin light chain kinase/MLCK aa 1850-1950 (C terminal). The exact sequence is proprietary.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP1458Y -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
- Anti-Myosin light chain kinase/MLCK antibody [EP1458Y] - BSA and Azide free (ab238429)
- PE Anti-Myosin light chain kinase/MLCK antibody [EP1458Y] (ab317912)
- APC Anti-Myosin light chain kinase/MLCK antibody [EP1458Y] (ab317953)
- Alexa Fluor® 488 Anti-Myosin light chain kinase/MLCK antibody [EP1458Y] (ab317994)
- Alexa Fluor® 594 Anti-Myosin light chain kinase/MLCK antibody [EP1458Y] (ab318036)
- Alexa Fluor® 555 Anti-Myosin light chain kinase/MLCK antibody [EP1458Y] (ab318078)
- Alexa Fluor® 647 Anti-Myosin light chain kinase/MLCK antibody [EP1458Y] (ab318119)
- Alexa Fluor® 750 Anti-Myosin light chain kinase/MLCK antibody [EP1458Y] (ab321713)
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab76092の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | (2) |
1/25000. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Use of HRP-conjugated or polymerized HRP secondary antibodies, stronger signals have been found using the polymerized HRP secondary. |
| 特記事項 |
|---|
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IHC-P
1/25000. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. Use of HRP-conjugated or polymerized HRP secondary antibodies, stronger signals have been found using the polymerized HRP secondary. |
ターゲット情報
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機能
Calcium/calmodulin-dependent enzyme implicated in smooth muscle contraction via phosphorylation of myosin light chains (MLC). Also regulates actin-myosin interaction through a non-kinase activty (By similarity). Implicated in the regulation of endothelial as well as vascular permeability. In the nervous system it has been shown to control the growth initiation of astrocytic processes in culture and to participate in transmitter release at synapses formed between cultured sympathetic ganglion cells. Critical participant in signaling sequences that result in fibroblast apoptosis. -
組織特異性
Smooth muscle and non-muscle isozymes are expressed in a wide variety of adult and fetal tissues and in cultured endothelium with qualitative expression appearing to be neither tissue- nor development-specific. Non-muscle isoform 2 is the dominant splice variant expressed in various tissues. Telokin has been found in a wide variety of adult and fetal tissues. -
配列類似性
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
Contains 1 fibronectin type-III domain.
Contains 9 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain. -
翻訳後修飾
MLCK is probably down-regulated by phosphorylation.
The C-terminus is deglutamylated by AGTPBP1/ CCP1, AGBL1/CCP4 and AGBL4/CCP6, leading to the formation of Myosin light chain kinase, smooth muscle, deglutamylated form. The consequences of C-terminal deglutamylation are unknown. - Information by UniProt
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参照データベース
- Entrez Gene: 4638 Human
- Entrez Gene: 107589 Mouse
- Entrez Gene: 288057 Rat
- Omim: 600922 Human
- SwissProt: Q15746 Human
- SwissProt: Q6PDN3 Mouse
- Unigene: 477375 Human
- Unigene: 33360 Mouse
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別名
- deglutamylated form antibody
- DKFZp686I10125 antibody
- EC 2.7.11.18 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain kinase/MLCK antibody [EP1458Y] (ab76092)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat colon tissue sections labelling Myosin light chain kinase/MLCK with purified ab76092 at 1/25000 dilution (0.005 µ?g/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain kinase/MLCK antibody [EP1458Y] (ab76092)](https://www.abcam.co.jp/ps/products/76/ab76092/Images/ab76092-7-antimyosin-lightchain-kinase-mlck-antibody-ep1458y-immunohistochemistry-colon-mouse.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain kinase/MLCK antibody [EP1458Y] (ab76092)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse colon tissue sections labelling Myosin light chain kinase/MLCK with purified ab76092 at 1/25000 dilution (0.005 µg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain kinase/MLCK antibody [EP1458Y] (ab76092)](https://www.abcam.co.jp/ps/products/76/ab76092/Images/ab76092-6-antimyosin-lightchain-kinase-mlck-antibody-ep1458y-immunohistochemistry-colon-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain kinase/MLCK antibody [EP1458Y] (ab76092)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue sections labelling Myosin light chain kinase/MLCK with purified ab76092 at 1/25000 dilution (0.005 µg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
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![Anti-Myosin light chain kinase/MLCK antibody [EP1458Y] (ab76092)](https://www.abcam.co.jp/ps/products/76/ab76092/Images/ab76092-5-benefits-of-recombinant-antibodies.png)
Anti-Myosin light chain kinase/MLCK antibody [EP1458Y] (ab76092)
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (57)
ab76092 は 57 報の論文で使用されています。
- Cai H et al. Heterozygous Actg2R257C mice mimic the phenotype of megacystis microcolon intestinal hypoperistalsis syndrome. Neurogastroenterol Motil 35:e14472 (2023). PubMed: 36264152
- Yang S et al. Internalization of apoptotic cells during efferocytosis requires Mertk-mediated calcium influx. Cell Death Dis 14:391 (2023). PubMed: 37391432
- Luo J et al. LTB4 Promotes Acute Lung Injury via Upregulating the PLCe-1/TLR4/NF-?B Pathway in One-Lung Ventilation. Dis Markers 2022:1839341 (2022). PubMed: 35059042
- Du J et al. N6-adenomethylation of GsdmC is essential for Lgr5+ stem cell survival to maintain normal colonic epithelial morphogenesis. Dev Cell 57:1976-1994.e8 (2022). PubMed: 35917813
- Liu L et al. Myocardin regulates exon usage in smooth muscle cells through induction of splicing regulatory factors. Cell Mol Life Sci 79:459 (2022). PubMed: 35913515