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リコンビナントRabMAb

Anti-Myosin light chain 3 抗体 [EPR4160] (ab108923)

Submit a review Submit a question References (2)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain 3 antibody [EPR4160] (ab108923)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain 3 antibody [EPR4160] (ab108923)
  • Western blot - Anti-Myosin light chain 3 antibody [EPR4160] (ab108923)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain 3 antibody [EPR4160] (ab108923)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain 3 antibody [EPR4160] (ab108923)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain 3 antibody [EPR4160] (ab108923)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain 3 antibody [EPR4160] (ab108923)
  • Anti-Myosin light chain 3 antibody [EPR4160] (ab108923)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4160] to Myosin light chain 3
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-Myosin light chain 3 antibody [EPR4160]
    Myosin light chain 3 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR4160] to Myosin light chain 3

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-Pmore details
    適用なし: Flow Cyt,ICC/IF or IP

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • human heart muscle lysate, human skeletal muscle lysate; human cardiac muscle tissue, human skeletal muscle tissue

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab108923の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 22 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 22 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
追加情報
Is unsuitable for Flow Cyt,ICC/IF or IP.

ターゲット情報

  • 機能

    Regulatory light chain of myosin. Does not bind calcium.

  • 関連疾患

    Defects in MYL3 are the cause of cardiomyopathy familial hypertrophic type 8 (CMH8) [MIM:608751]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death. CMH8 inheritance can be autosomal dominant or recessive.
    Defects in MYL3 are the cause of cardiomyopathy familial hypertrophic with mid-left ventricular chamber type 1 (MVC1) [MIM:608751]. MVC1 is a very rare variant of familial hypertrophic cardiomyopathy, characterized by mid-left ventricular chamber thickening.

  • 配列類似性

    Contains 3 EF-hand domains.

  • 翻訳後修飾

    The N-terminus is blocked.
    N-terminus is methylated by METTL11A/NTM1.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • Cardiac myosin light chain 1 antibody
    • CMH8 antibody
    • CMLC1 antibody
    • ELC of myosin antibody
    • Essential light chain of myosin antibody
    • MLC1SB antibody
    • MLC1V antibody
    • MYL3 antibody
    • MYL3_HUMAN antibody
    • Myosin light chain 1 antibody
    • Myosin light chain 1 slow twitch muscle B/ventricular isoform antibody
    • Myosin light chain 1 slow, B antibody
    • Myosin light chain 3 antibody
    • Myosin, light chain 1, ventricular antibody
    • myosin, light chain 3, alkali; ventricular, skeletal, slow antibody
    • myosin, light polypeptide 3, alkali antibody
    • myosin, light polypeptide 3, alkali; ventricular, skeletal, slow antibody
    • OTTHUMP00000165922 antibody
    • Slow skeletal ventricular myosin alkali light chain 3 antibody
    • slow-twitch muscle B/ventricular isoform antibody
    • Ventricular/slow twitch myosin alkali light chain antibody
    • VLC1 antibody
    see all

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain 3 antibody [EPR4160] (ab108923)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain 3 antibody [EPR4160] (ab108923)

    Immunohistochemical staining of paraffin-embedded human cardiac muscle using ab108923 at a dilution of 1/100.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain 3 antibody [EPR4160] (ab108923)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain 3 antibody [EPR4160] (ab108923)

    Immunohistochemical staining of paraffin-embedded human skeletal muscle tissue using ab108923 at a diltion of 1/100.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-Myosin light chain 3 antibody [EPR4160] (ab108923)
    Western blot - Anti-Myosin light chain 3 antibody [EPR4160] (ab108923)
    All lanes : Anti-Myosin light chain 3 antibody [EPR4160] (ab108923) at 1/1000 dilution

    Lane 1 : human heart muscle tissue lysate
    Lane 2 : human skeletal muscle tissue lysate

    Lysates/proteins at 10 µg/ml per lane.

    Predicted band size: 22 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain 3 antibody [EPR4160] (ab108923)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain 3 antibody [EPR4160] (ab108923)

    ab108923 showing negative staining in Normal brain tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain 3 antibody [EPR4160] (ab108923)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain 3 antibody [EPR4160] (ab108923)

    ab108923 showing negative staining in Normal liver tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain 3 antibody [EPR4160] (ab108923)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain 3 antibody [EPR4160] (ab108923)

    ab108923 showing negative staining in Normal tonsil tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain 3 antibody [EPR4160] (ab108923)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain 3 antibody [EPR4160] (ab108923)

    ab108923 showing negative staining in Normal breast tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-Myosin light chain 3 antibody [EPR4160] (ab108923)
    Anti-Myosin light chain 3 antibody [EPR4160] (ab108923)

参考文献 (2)

ab108923 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab108923 は 2 報の論文で使用されています。

  • Turco MP  et al. Periurethral muscle-derived mononuclear cell injection improves urethral sphincter restoration in rats. Neurourol Urodyn 36:2011-2018 (2017). PubMed: 28346707
  • Ren Y  et al. Potential of adipose-derived mesenchymal stem cells and skeletal muscle-derived satellite cells for somatic cell nuclear transfer mediated transgenesis in Arbas Cashmere goats. PLoS One 9:e93583 (2014). ICC ; Goat . PubMed: 24699686

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