Anti-Myosin Light Chain 2 抗体 (ab79935)
Key features and details
- Rabbit polyclonal to Myosin Light Chain 2
- Suitable for: WB, IHC-P, IP
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Myosin Light Chain 2 antibody
Myosin Light Chain 2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Myosin Light Chain 2 -
由来種
Rabbit -
特異性
From September 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
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アプリケーション
適用あり: WB, IHC-P, IPmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Sheep, Cow, Dog, Pig
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免疫原
Synthetic peptide corresponding to Human Myosin Light Chain 2 aa 100 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab88166) -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab79935の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa).
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| IHC-P |
Use a concentration of 5 µg/ml.
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| IP |
Use a concentration of 5 µg/ml.
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| 特記事項 |
|---|
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa). |
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IHC-P
Use a concentration of 5 µg/ml. |
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IP
Use a concentration of 5 µg/ml. |
ターゲット情報
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関連疾患
Defects in MYL2 are the cause of cardiomyopathy familial hypertrophic type 10 (CMH10) [MIM:608758]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
Defects in MYL2 are the cause of cardiomyopathy familial hypertrophic with mid-left ventricular chamber type 2 (MVC2) [MIM:608758]. MVC2 is a very rare variant of familial hypertrophic cardiomyopathy, characterized by mid-left ventricular chamber thickening. -
配列類似性
Contains 3 EF-hand domains. -
翻訳後修飾
N-terminus is methylated by METTL11A/NTM1. - Information by UniProt
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参照データベース
- Entrez Gene: 505519 Cow
- Entrez Gene: 403614 Dog
- Entrez Gene: 4633 Human
- Entrez Gene: 17906 Mouse
- Entrez Gene: 363925 Rat
- Omim: 160781 Human
- SwissProt: Q3SZE5 Cow
- SwissProt: P10916 Human
see all -
別名
- Cardiac myosin light chain-2 antibody
- Cardiac ventricular myosin light chain 2 antibody
- CMH10 antibody
see all
画像
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Immunoprecipitation - Anti-Myosin Light Chain 2 antibody (ab79935)
Myosin Light Chain 2 was immunoprecipitated using 0.5mg Mouse Heart tissue, 5µg of Rabbit polyclonal to Myosin Light Chain 2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Mouse Heart tissue lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab79935.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 19kDa; Myosin Light Chain 2
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin Light Chain 2 antibody (ab79935)IHC image of Myosin light chain 2 antibody staining in a section of formalin-fixed paraffin-embedded normal mouse heart performed on a Leica BONDTM system using the standard protocol. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab79935, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Western blot - Anti-Myosin Light Chain 2 antibody (ab79935)All lanes : Anti-Myosin Light Chain 2 antibody (ab79935) at 1 µg/ml
Lane 1 : Human heart tissue lysate - total protein (ab29431)
Lane 2 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lane 3 : Heart (Mouse) Tissue Lysate
Lane 4 : Heart (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 19 kDa
Observed band size: 19 kDa
Additional bands at: 17 kDa, 8 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 15 minutes
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (34)
ab79935 は 34 報の論文で使用されています。
- Chapotte-Baldacci CA et al. Biophysical properties of NaV1.5 channels from atrial-like and ventricular-like cardiomyocytes derived from human induced pluripotent stem cells. Sci Rep 13:20685 (2023). PubMed: 38001331
- Bornstein B et al. Molecular characterization of the intact mouse muscle spindle using a multi-omics approach. Elife 12:N/A (2023). PubMed: 36744866
- Yap L et al. Pluripotent stem cell-derived committed cardiac progenitors remuscularize damaged ischemic hearts and improve their function in pigs. NPJ Regen Med 8:26 (2023). PubMed: 37236990
- Wu X et al. Cardiac Development in the Presence of Cadmium: An in Vitro Study Using Human Embryonic Stem Cells and Cardiac Organoids. Environ Health Perspect 130:117002 (2022). PubMed: 36321828
- Gu Z et al. A Uterus-Inspired Niche Drives Blastocyst Development to the Early Organogenesis. Adv Sci (Weinh) 9:e2202282 (2022). PubMed: 35843885