Anti-Myosin Light Chain 2 抗体 (ab48003)

Thank you for your reply.

I can confirm that the immunogen for ab48003 was a synthetic peptide corresponding to amino acids 12 to 27 of Human Myosin Light Chain 2 (GANSNVFSMFEQTQIQ).

The antibody therefore binds to this region.

I can only confirm that the antibody bindsboth unphosphorylated and monophosphorylated forms of the protein. We assume this binding is equal, but this has not been tested experimentally.

I hope this information is helpful and wish you good luck with your research.

We recommend use of a 5% non fat dry milk/TBS blocking and incubation solution. We also recommend a starting dilution for the antibody within the range 1/500 - 1/2000. This is just a suggested starting range for the product. The optimal antibody dilution may ultimately be outside of this range and should be determined in the lab.

I have issued a free of charge replacement for one vial of ab92721. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.  

DISCOUNT CODE: *** Expiration date: April 6th, 2012 I am very pleased to hear you would like to accept our offer and test ab48003 in ICC on mouse. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for ICC on mouse cells and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.