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AB8070

Anti-Myosin 抗体 [414]

Anti-Myosin antibody [414]

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(1 Publication)

Mouse Monoclonal Fast Myosin Skeletal Heavy chain antibody. Suitable for ICC, WB, IHC-P, IHC-Fr and reacts with Chicken samples. Cited in 1 publication.

別名を表示する

Myosin-1, Myosin heavy chain 1, Myosin heavy chain 2x, Myosin heavy chain IIx/d, MyHC-2x, MyHC-IIx/d, MYH1

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

414

アイソタイプ

IgM

軽鎖のタイプ

unknown

キャリアフリー

No

交差種

Chicken

アプリケーション

IHC-P, IHC-Fr, ICC, WB

applications

特異性

This monoclonal antibody reacts in immunoblots specific with myosin and is specific to the 75kD of Myosin. Does not react with alpha-cardiac myosin. Does not react with the light-chain but with the last 75 kD of the myosin molecule. These 75 kD are located on a myosin-common site-chain.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Chicken": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p>Use at 1/5 - 1/10.(Only with microwave oven pretreatment. Based on the Indirect method, with a normal ABC method the dilution can be 5-10 times higher). Dilute immediately before use with PBS or Tris.</p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" } } }

出荷温度及び保存条件

製品の状態
Liquid
精製度
Tissue culture supernatant
バッファー組成
pH: 7.3 Preservative: 0.1% Sodium azide Constituents: 1% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Myosin is a molecular motor protein responsible for converting chemical energy into mechanical force facilitating muscle contraction and cellular movement. Also known as the myosin heavy chain (MHC) or MYH myosin has a commonly referenced heavy chain Myosin heavy chain 1 (MYH1) with a molecular mass of roughly 200 kDa. Myosin is broadly expressed in muscle tissues including skeletal cardiac and smooth muscles and appears in various isoforms depending on the specific type of muscle tissue.
Biological function summary

Myosin interacts intricately with actin filaments to form a complex that executes muscle contraction and cell motility. Myosin heads bind to actin filaments employing ATP hydrolysis to generate motion. This protein is a fundamental component of the sarcomere the structural unit of muscle fibers and works in concert with other proteins like tropomyosin and troponin to regulate muscle contraction.

Pathways

Myosin has a significant role in the muscle contraction and cell motility pathways. Myosin and actin interaction is central to the sliding filament theory which is an essential part of the muscle contraction pathway. Myosin is also involved in non-muscle cell movement through the actin-myosin contractile ring in the process of cytokinesis. Myosin interacts with proteins such as actin and ATP in these pathways to facilitate cellular processes.

Myosin has connections to conditions like hypertrophic cardiomyopathy and various myopathies. Mutations in the myosin heavy chain can lead to hypertrophic cardiomyopathy affecting the heart's ability to pump blood effectively. Additionally disruptions in myosin function can contribute to muscular diseases impairing muscle strength and function. Myosin's interaction with actin and related sarcomeric proteins is pivotal in these pathological scenarios often resulting in altered muscle dynamics and associated clinical manifestations.

製品プロトコール

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ターゲットの情報

Muscle contraction.
See full target information MYH1

文献 (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 26: PubMed40869109

2025

Exploring the Heterogeneity of Cancer-Associated Fibroblasts via Development of Patient-Derived Cell Culture of Breast Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Ilyina,Anastasia Leonteva,Ekaterina Berezutskaya,Maria Abdurakhmanova,Mikhail Ermakov,Sergey Mishinov,Elena Kuligina,Sergey Vladimirov,Maria Bogachek,Vladimir Richter,Anna Nushtaeva
View all publications

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