AB201228

Anti-MYOM1 抗体 [EPR17322]

Name: Anti-MYOM1 抗体 [EPR17322] (ab201228) | Abcam
Brand: Abcam Limited
SKU: AB201228
Price: 56000 JPY
Availability: InStock
Rating: 5 (1 reviews)

Anti-MYOM1 antibody [EPR17322]

RabMAb
Recombinant
詳細を見る

(1 Review)

(4 Publications)

Rabbit Recombinant Monoclonal MYOM1 antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 4 publications.

別名を表示する

Myomesin-1, 190 kDa connectin-associated protein, 190 kDa titin-associated protein, Myomesin family member 1, MYOM1

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYOM1 antibody [EPR17322] (AB201228)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYOM1 antibody [EPR17322] (AB201228)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling MYOM1 with ab201228 at 1/400 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Human tonsil tissue is a negative control for MYOM1. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYOM1 antibody [EPR17322] (AB201228)

Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling MYOM1 with ab201228 at 1/400 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasmic staining on Human skeletal muscle tissue is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYOM1 antibody [EPR17322] (AB201228)

Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labeling MYOM1 with ab201228 at 1/400 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasmic staining on mouse skeletal muscle tissue is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYOM1 antibody [EPR17322] (AB201228)

Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labeling MYOM1 with ab201228 at 1/400 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasmic staining on rat skeletal muscle tissue is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-MYOM1 antibody [EPR17322] (AB201228)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-MYOM1 antibody [EPR17322] (ab201228) at 1/20000 dilution

All lanes:

Human fetal heart tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 188 kDa

Observed band size: 188 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-MYOM1 antibody [EPR17322] (AB201228)

5% NFDM/TBST : Blocking and diluting buffer.

All lanes:

Western blot - Anti-MYOM1 antibody [EPR17322] (ab201228) at 1/20000 dilution

Lane 1:

Mouse heart lysate at 20 µg

Lane 2:

Mouse muscle lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 188 kDa

Observed band size: 188 kDa

false

Exposure time: 1min

Supplier Data

Western blot - Anti-MYOM1 antibody [EPR17322] (AB201228)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-MYOM1 antibody [EPR17322] (ab201228) at 1/20000 dilution

Lane 1:

Rat heart lysate at 20 µg

Lane 2:

Rat brain lysate at 20 µg

Lane 3:

Rat kidney lysate at 20 µg

Lane 4:

C6 (Rat glial tumor cells) whole cell lysate at 20 µg

Lane 5:

PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 188 kDa

Observed band size: 188 kDa

false

Exposure time: 15s

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR17322

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Rat, Human

アプリケーション

WB, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/20000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/400", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/20000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/400", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/20000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/400", "IHCP-species-notes": "" } } }

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製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Conditional Ambient

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

MYOM1 also known as myomesin 1 is a protein with a molecular mass of approximately 185 kDa. It plays a mechanical role in muscle cells where it contributes to the M-band region of the sarcomere. MYOM1 is important in the structural integrity and elasticity of the myofibrils. Expression of this protein is specifically seen in cardiac and skeletal muscle tissues indicating its significance in muscle function.

Biological function summary

The function of MYOM1 involves its contribution to sarcomere organization and stability. It acts as a scaffold in the M-band where it forms complexes with other sarcomeric proteins. These complexes include titin and muscle-specific kinase 1 which help maintain sarcomere structure during muscle contraction and stretch. MYOM1's interaction with these proteins is essential for muscle elasticity and proper muscle function.

Pathways

MYOM1 operates within the muscle contraction pathway and the sarcomere organization pathway. It interacts closely with titin which is a giant protein essential for muscle elasticity and with beta-catenin a protein involved in cell signaling and cytoskeleton organization. The interaction and the pathways help maintain muscle function and structural integrity ensuring proper muscle responses to physiological demands.

MYOM1 is linked to the development of myopathies particularly myosin storage myopathy and hypertrophic cardiomyopathy. These conditions are associated with mutations or malfunctions in proteins such as titin and myosin which are part of MYOM1's network. Understanding the connection between MYOM1 and these proteins helps in unraveling the pathology of these muscle disorders presenting potential targets for therapeutic interventions.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Major component of the vertebrate myofibrillar M band. Binds myosin, titin, and light meromyosin. This binding is dose dependent.

See full target information MYOM1

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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Circulation 146:1674-1693 PubMed36321451

2022

Pathogenesis of Cardiomyopathy Caused by Variants in , an Essential Pseudokinase in the Cardiomyocyte Nucleus and Sarcomere.

Applications

Unspecified application

Species

Unspecified reactive species

Radhika Agarwal,Hiroko Wakimoto,Joao A Paulo,Qi Zhang,Daniel Reichart,Christopher Toepfer,Arun Sharma,Angela C Tai,Mingyue Lun,Joshua Gorham,Steven R DePalma,Steven P Gygi,J G Seidman,Christine E Seidman

Acta biochimica Polonica 69:587-592 PubMed35977075

2022

MiR-135a is highly expressed and aggravates inflammatory response in sepsis by targeting MYOM1.

Applications

Unspecified application

Species

Unspecified reactive species

Sheng Chen,Han Zhang,Haoming Li

Frontiers in genetics 13:902329 PubMed35938015

2022

Analysis of characteristic genes and ceRNA regulation mechanism of endometriosis based on full transcriptional sequencing.

Applications

Unspecified application

Species

Unspecified reactive species

Chengmao Xie,Ziran Yin,Yong Liu

Cardiovascular pathology : the official journal of the Society for Cardiovascular Pathology 26:12-20 PubMed27837684

2016

Altered protein levels in the isolated extracellular matrix of failing human hearts with dilated cardiomyopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Joshua L DeAguero,Elizabeth N McKown,Liwen Zhang,Jeremy Keirsey,Edgar G Fischer,Von G Samedi,Benjamin D Canan,Ahmet Kilic,Paul M L Janssen,Dawn A Delfín

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MYOM1 抗体 [EPR17322]

Anti-MYOM1 antibody [EPR17322]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYOM1 antibody [EPR17322] (AB201228)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYOM1 antibody [EPR17322] (AB201228)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYOM1 antibody [EPR17322] (AB201228)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYOM1 antibody [EPR17322] (AB201228)

Western blot - Anti-MYOM1 antibody [EPR17322] (AB201228)

Western blot - Anti-MYOM1 antibody [EPR17322] (AB201228)

Western blot - Anti-MYOM1 antibody [EPR17322] (AB201228)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

代替製品

Primary Antibodies

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (4)

Abcam product promise