Name: Anti-Myoglobin antibody [EP3081Y]
Brand: RabMAb
SKU: ab77232
Rating: 5 (1 reviews)

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Western blot - Anti-Myoglobin antibody [EP3081Y] (ab77232)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP3081Y] to Myoglobin
Suitable for: WB, IHC-P, ICC/IF
Reacts with: Mouse, Rat, Human

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 750 APC Carrier Free HRP PE

製品名

Anti-Myoglobin antibody [EP3081Y]
Myoglobin 一次抗体製品一覧
製品の詳細

Rabbit monoclonal [EP3081Y] to Myoglobin
由来種

Rabbit
アプリケーション

適用あり: WB, IHC-P, ICC/IFmore details
種交差性

交差種: Mouse, Rat, Human
免疫原

Synthetic peptide within Human Myoglobin aa 100-200 (C terminal). The exact sequence is proprietary.
Database link: P02144
ポジティブ･コントロール
- Human, rat and mouse muscle tissue lysates; human heart muscle tissue.
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

製品の状態

Liquid
保存方法

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
解離定数（K_D 値）

K_D = 1.78 x 10 ^-10 M
Learn more about K_D
バッファー

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
Concentration information loading...
精製度

Protein A purified
ポリ/モノ

モノクローナル
クローン名

EP3081Y
アイソタイプ

IgG
研究分野
- Neuroscience
- Processes

The Abpromise guarantee

Abpromise保証は、次のテスト済みアプリケーションにおけるab77232の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション	Abreviews	特記事項
WB	(1)	1/1000 - 1/5000. Predicted molecular weight: 17 kDa.
IHC-P		1/250 - 1/500. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. Use of an HRP/AP polymerized secondary antibody is recommended. See IHC antigen retrieval protocols.
ICC/IF		Use at an assay dependent concentration.

特記事項
WB 1/1000 - 1/5000. Predicted molecular weight: 17 kDa.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. Use of an HRP/AP polymerized secondary antibody is recommended. See IHC antigen retrieval protocols.
ICC/IF Use at an assay dependent concentration.

機能

Serves as a reserve supply of oxygen and facilitates the movement of oxygen within muscles.
配列類似性

Belongs to the globin family.
Target information above from: UniProt accession P02144 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
参照データベース
- Entrez Gene: 4151 Human
- Entrez Gene: 17189 Mouse
- Entrez Gene: 59108 Rat
- Omim: 160000 Human
- SwissProt: P02144 Human
- SwissProt: P04247 Mouse
- SwissProt: Q9QZ76 Rat
- Unigene: 517586 Human
- Unigene: 404074 Mouse
- Unigene: 40511 Rat
see all
別名
- MB antibody
- MGC13548 antibody
- MYG_HUMAN antibody
- Myoglobin antibody

Western blot - Anti-Myoglobin antibody [EP3081Y] (ab77232)

All lanes : Anti-Myoglobin antibody [EP3081Y] (ab77232) at 1/5000 dilution (Purified)

Lane 1 : Human muscle lysate
Lane 2 : Mouse muscle lysate
Lane 3 : Rat muscle lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 17 kDa
Observed band size: 17 kDa

Exposure time: 1 second

Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myoglobin antibody [EP3081Y] (ab77232)

Purified ab77232 staining Myoglobin in Human cardiac muscle tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde; antigen retrieval was by heat mediation in a Tris/EDTA buffer (ab93684 Tris/EDTA buffer, pH 9.0). Samples were incubated with primary antibody (1/1000). A HRP-conjugated Goat Anti-Rabbit IgG H&L (ready to use) was used as the secondary antibody. Counterstained with Hematoxylin.
Immunocytochemistry/ Immunofluorescence - Anti-Myoglobin antibody [EP3081Y] (ab77232)

Purified ab77232 staining Myoglobin in the C2C12 (mouse myoblasts myoblast) cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol. Samples were incubated with primary antibody and an Alexa Fluor^® 488-conjugated Goat anti-rabbit IgG (ab150077) was used as the secondary antibody. Counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) and DAPI.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myoglobin antibody [EP3081Y] (ab77232)

Purified ab77232 staining Myoglobin in Mouse cardiac muscle tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde; antigen retrieval was by heat mediation in a Tris/EDTA buffer (ab93684 Tris/EDTA buffer, pH 9.0). Samples were incubated with primary antibody (1/1000). A HRP-conjugated Goat Anti-Rabbit IgG H&L (ready to use) was used as the secondary antibody. Counterstained with Hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myoglobin antibody [EP3081Y] (ab77232)

Purified ab77232 staining Myoglobin in Rat cardiac muscle tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde; antigen retrieval was by heat mediation in a Tris/EDTA buffer (ab93684 Tris/EDTA buffer, pH 9.0). Samples were incubated with primary antibody (1/1000). A HRP-conjugated Goat Anti-Rabbit IgG H&L (ready to use) was used as the secondary antibody. Counterstained with Hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myoglobin antibody [EP3081Y] (ab77232)

Unpurified ab77232 showing positive staining in human Skeletal muscle tissue.

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Western blot - Anti-Myoglobin antibody [EP3081Y] (ab77232)This image is courtesy of an anonymous Abreview

Western blot analysis of Pig skeletal muscle whole tissue lysate (10μg/lane) labelling Myoglobin with unpurified ab77232 at 1/5000 in 1x PBS + 0.1% Tween-20 for 17 hours at 4ºC. A IRDye^® 680-conjugated Donkey anti-rabbit polyclonal (1/40000) was used as the secondary antibody.
See Abreview
Western blot - Anti-Myoglobin antibody [EP3081Y] (ab77232)

All lanes : Anti-Myoglobin antibody [EP3081Y] (ab77232) at 1/5000 dilution (Unpurified)

Lane 1 : Human heart muscle lysate
Lane 2 : Mouse heart muscle lysate
Lane 3 : Rat heart muscle lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 17 kDa
Observed band size: 17 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myoglobin antibody [EP3081Y] (ab77232)

Unpurified ab77232, at a 1/250 dilution, staining Myoglobin in paraffin embedded human heart muscle by Immunohistochemistry.

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myoglobin antibody [EP3081Y] (ab77232)

Unpurified ab77232 showing negative staining in Normal human brain tissue.

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myoglobin antibody [EP3081Y] (ab77232)

Unpurified ab77232 showing negative staining in Normal human kidney tissue.

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myoglobin antibody [EP3081Y] (ab77232)

Unpurified ab77232 showing negative staining in Normal human liver tissue.

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
OI-RD Scanning - Anti-Myoglobin antibody [EP3081Y] (ab77232)

Produced using unpurified ab77232.

Equilibrium disassociation constant (K_D)
Learn more about K_D

Click here to learn more about K_D
Anti-Myoglobin antibody [EP3081Y] (ab77232)

Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

ab77232 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab77232 は 31 報の論文で使用されています。

Yu M et al. lncRNA GPRC5D-AS1 as a ceRNA inhibits skeletal muscle aging by regulating miR-520d-5p. Aging (Albany NY) 15:13980-13997 (2023). PubMed: 38100482
Aboouf MA et al. Pro-Apoptotic and Anti-Invasive Properties Underscore the Tumor-Suppressing Impact of Myoglobin on a Subset of Human Breast Cancer Cells. Int J Mol Sci 23:N/A (2022). PubMed: 36232784
Majerczak J et al. Nitrite Concentration in the Striated Muscles Is Reversely Related to Myoglobin and Mitochondrial Proteins Content in Rats. Int J Mol Sci 23:N/A (2022). PubMed: 35269826
Liu F et al. HDAC9 exacerbates myocardial infarction via inactivating Nrf2 pathways. J Pharm Pharmacol N/A:N/A (2021). PubMed: 33963859
Blackburn ML et al. On the potential role of globins in brown adipose tissue: a novel conceptual model and studies in myoglobin knockout mice. Am J Physiol Endocrinol Metab 321:E47-E62 (2021). PubMed: 33969705

View all Publications for this product

レビューと Q&A

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レビューを送る質問を送る

1-2 of 2 Abreviews or Q&A

Application

Western blot

Loading amount

10 µg

Gel Running Conditions

Reduced Denaturing (14% 37.5:1)

Sample

Pig Tissue lysate - whole (Skeletal Muscle)

Specification

Skeletal Muscle

Blocking step

Thermo Starting Block 37542 as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Sep 16 2013

Question

What is the concentration of lot GR52810?

Abcam community

Verified customer

Asked on Jan 23 2012

Answer

Thank you for contacting us.

The IgG concentration ab77232, lot GR52810, is 0.100 mg/mL.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Abcam Scientific Support

Answered on Jan 23 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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Key features and details

Related conjugates and formulations

製品の概要

製品名

製品の詳細

由来種

アプリケーション

種交差性

免疫原

ポジティブ･コントロール

特記事項

製品の特性

製品の状態

保存方法

解離定数（KD 値）

バッファー

精製度

ポリ/モノ

クローン名

アイソタイプ

研究分野

関連製品

Alternative Versions

Compatible Secondaries

Isotype control

Recombinant Protein

アプリケーション

The Abpromise guarantee

ターゲット情報

機能

配列類似性

参照データベース

別名

画像

プロトコール

データシートおよび資料

SDS download

Datasheet download

参考文献 (31)

レビューと Q&A

Western blot abreview for Anti-Myoglobin antibody [EP3081Y]

解離定数（K_D 値）