Anti-Myoglobin 抗体 [6H8B5] (ab47702)
![Western blot - Anti-Myoglobin antibody [6H8B5] (ab47702)](https://www.abcam.co.jp/ps/products/47/ab47702/Images/ab47702-60288-anti-myoglobin-antibody-6h8b5-western-blot.jpg "Western blot - Anti-Myoglobin antibody [6H8B5] (ab47702)")
Key features and details
- Mouse monoclonal [6H8B5] to Myoglobin
- Suitable for: WB, IHC-P
- Reacts with: Human, Recombinant fragment
- Isotype: IgG1
製品の概要
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製品名
Anti-Myoglobin antibody [6H8B5]
Myoglobin 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [6H8B5] to Myoglobin -
由来種
Mouse -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human, Recombinant fragment -
免疫原
Ni-NTA purified truncated recombinant Myoglobin expressed in E. Coli strain BL21 (DE3)
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ポジティブ･コントロール
- IHC-P: Human skeletal muscle tissue.
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特記事項
This product was changed from ascites to supernatant. Lot no’s high than GR232969-1 are from Tissue Culture Supernatant
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Protein G purified -
特記事項（精製）
Purified from tissue culture supernatant. -
ポリ/モノ
モノクローナル -
クローン名
6H8B5 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab47702の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/2000. Detects a band of approximately 39 kDa (predicted molecular weight: 17 kDa).
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| IHC-P |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
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WB
1/500 - 1/2000. Detects a band of approximately 39 kDa (predicted molecular weight: 17 kDa). |
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IHC-P
Use at an assay dependent concentration. |
ターゲット情報
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機能
Serves as a reserve supply of oxygen and facilitates the movement of oxygen within muscles. -
配列類似性
Belongs to the globin family. - Information by UniProt
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参照データベース
- Entrez Gene: 4151 Human
- Omim: 160000 Human
- SwissProt: P02144 Human
- Unigene: 517586 Human
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別名
- MB antibody
- MGC13548 antibody
- MYG_HUMAN antibody
- Myoglobin antibody
画像
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Western blot - Anti-Myoglobin antibody [6H8B5] (ab47702)Anti-Myoglobin antibody [6H8B5] (ab47702) + Truncated Myoglobin recombinant protein (2-154aa) (Tagged)
Predicted band size: 17 kDa
Observed band size: 39 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myoglobin antibody [6H8B5] (ab47702)](https://www.abcam.co.jp/ps/products/47/ab47702/Images/ab47702-60287-anti-myoglobin-antibody-6h8b5-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myoglobin antibody [6H8B5] (ab47702)Ab47702 staining human Myoglobin in human skeletal muscle tissue, showing cytoplasmic localization, with DAB staining.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab47702 は 1 報の論文で使用されています。
- Perrone CE et al. Methionine restriction effects on mitochondrial biogenesis and aerobic capacity in white adipose tissue, liver, and skeletal muscle of F344 rats. Metabolism 59:1000-11 (2010). WB ; Rat . PubMed: 20045141