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AB178386

Anti-Myoferlin 抗体 [EPR18887]

Anti-Myoferlin antibody [EPR18887]

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Rabbit Recombinant Monoclonal Myoferlin antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples.

別名を表示する

FER1L3, KIAA1207, MYOF, Myoferlin, Fer-1-like protein 3

6 Images
Western blot - Anti-Myoferlin antibody [EPR18887] (AB178386)
  • WB

Lab

Western blot - Anti-Myoferlin antibody [EPR18887] (AB178386)

Lanes 1-4 : Merged signal (red and green). Green - ab178386 observed at 250,180 kDa. Red - loading control ab7291 observed at 50 kDa.

ab178386 Anti-Myoferlin antibody [EPR18887] was shown to specifically react with Myoferlin in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265782 (knockout cell lysate ab257547) was used. Wild-type and Myoferlin knockout samples were subjected to SDS-PAGE. ab178386 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Myoferlin antibody [EPR18887] (ab178386) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

MYOF knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human MYOF (Myoferlin) knockout HeLa cell line (<a href='/products/cell-lines/human-myof-myoferlin-knockout-hela-cell-line-ab265782'>ab265782</a>)

Lane 3:

MDA-MB-231 cell lysate at 20 µg

Lane 4:

SK-BR-3 cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 235 kDa

Observed band size: 180 kDa,250 kDa

false

Immunocytochemistry/ Immunofluorescence - Anti-Myoferlin antibody [EPR18887] (AB178386)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Myoferlin antibody [EPR18887] (AB178386)

Immunofluorescent analysis of 100% methanol-fixed C2C12 (mouse myoblast cell line) cells labeling Myoferlin with ab178386 at 1/100 dilution followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on C2C12 cells. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (Alexa Fluor® 594 Goat anti-Mouse secondary) at 1/1000 dilution (red).

The negative controls are as follows :
-ve control 1 : ab178386 at 1/100 dilution followed by ab150120 (Alexa Fluor® 594 Goat anti-Mouse secondary) at 1/1000 dilution.

-ve control 2 : ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor® 488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

Flow Cytometry (Intracellular) - Anti-Myoferlin antibody [EPR18887] (AB178386)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Myoferlin antibody [EPR18887] (AB178386)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Myoferlin with ab178386 at 1/400 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.

Immunocytochemistry/ Immunofluorescence - Anti-Myoferlin antibody [EPR18887] (AB178386)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Myoferlin antibody [EPR18887] (AB178386)

Immunofluorescent analysis of 100% methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Myoferlin with ab178386 at 1/100 dilution followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HeLa cells. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (Alexa Fluor® 594 Goat anti-Mouse secondary) at 1/1000 dilution (red).

The negative controls are as follows :
-ve control 1 : ab178386 at 1/100 dilution followed by ab150120 (Alexa Fluor® 594 Goat anti-Mouse secondary) at 1/1000 dilution.

-ve control 2 : ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor® 488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

Western blot - Anti-Myoferlin antibody [EPR18887] (AB178386)
  • WB

Supplier Data

Western blot - Anti-Myoferlin antibody [EPR18887] (AB178386)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : Lane 1 : 3 minutes; Lane 2/4 : 10 seconds; Lane 3 : 3 seconds.

All lanes:

Western blot - Anti-Myoferlin antibody [EPR18887] (ab178386) at 1/2000 dilution

Lane 1:

Rat kidney lysate at 10 µg

Lane 2:

C6 (rat glial tumor cell line) whole cell lysate at 10 µg

Lane 3:

NIH/3T3 (mouse embryonic fibroblast cell line) whole cell lysate at 10 µg

Lane 4:

C2C12 (mouse myoblast cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 235 kDa

Observed band size: 180 kDa,250 kDa

false

Western blot - Anti-Myoferlin antibody [EPR18887] (AB178386)
  • WB

Supplier Data

Western blot - Anti-Myoferlin antibody [EPR18887] (AB178386)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure times : Lane 1/5 : 10 seconds; Lane 2 : 15 seconds; Lane 3/4 : 3 minutes.

The molecular weight observed is consistent with what has been described in the literature (PMID : 22135466, PMID : 23499551).

Lanes 1 - 2:

Western blot - Anti-Myoferlin antibody [EPR18887] (ab178386) at 1/20000 dilution

Lanes 3 - 5:

Western blot - Anti-Myoferlin antibody [EPR18887] (ab178386) at 1/2000 dilution

Lane 1:

MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 10 µg

Lane 2:

MDA-MB-231 (human breast adenocarcinoma cell line) whole cell lysate at 10 µg

Lane 3:

Human fetal kidney lysate at 10 µg

Lane 4:

Human fetal heart lysate at 10 µg

Lane 5:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 10 µg

Secondary

Lanes 1 - 3:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Lanes 4 - 5:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/100000 dilution

Predicted band size: 235 kDa

Observed band size: 180 kDa,250 kDa

false

関連する標識済み抗体及び組成の異なる製品 (1)

  • Carrier free

    Anti-Myoferlin antibody [EPR18887] - BSA and Azide free

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR18887

アイソタイプ

IgG

キャリアフリー

No

交差種

Mouse, Rat, Human

アプリケーション

WB, ICC/IF, Flow Cyt (Intra)

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/400", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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製品の詳細

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Myoferlin alternatively known as MYOF is a member of the ferlin family proteins and plays a mechanical role in membrane repair and fusion. It has a molecular mass of approximately 230 kDa. Myoferlin expresses mainly in muscle and endothelial tissues. Its function is critical during muscle development and regeneration as it participates in the fusion of myoblasts into mature myotubes. Also Myoferlin is involved in the repair of damaged sarcolemmal membranes in muscle cells.
Biological function summary

The protein supports membrane dynamics and stabilization. Myoferlin is not part of a large complex but interacts with other proteins at the cellular membrane. It regulates cellular processes such as proliferation differentiation and endocytosis. In muscle cells it assists in the organization and function of the cytoskeleton maintaining proper cellular architecture and signaling.

Pathways

Myoferlin engages in critical roles in pathways related to cell membrane repair and trafficking. It associates with pathways like the myogenesis pathway and integrin signaling. In these pathways it interacts with other proteins such as dysferlin and caveolin-3 which facilitate membrane repair and receptor localization respectively. Myoferlin ensures efficient cellular response to membrane damage maintaining tissue integrity.

Myoferlin is connected to muscular dystrophies and cancer. It correlates with the progression of muscular disorder due to its deficiency in membrane repair mechanisms. Additionally in cancer its expression levels relate to tumor invasion and metastasis involving interactions with proteins like E-cadherin and MMP-9. Investigating Myoferlin's role can aid in understanding its potential as a therapeutic target in these diseases.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Calcium/phospholipid-binding protein that plays a role in the plasmalemma repair mechanism of endothelial cells that permits rapid resealing of membranes disrupted by mechanical stress. Involved in endocytic recycling. Implicated in VEGF signal transduction by regulating the levels of the receptor KDR (By similarity).
See full target information MYOF
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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。
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