Anti-MyoD1 抗体 [5.2F] (ab16148)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MyoD1 antibody [5.2F] (ab16148)](https://www.abcam.co.jp/ps/products/16/ab16148/Images/ab16148-24867-anti-myod1-antibody-52f-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MyoD1 antibody [5.2F] (ab16148)")
Key features and details
- Mouse monoclonal [5.2F] to MyoD1
- Suitable for: Sandwich ELISA, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG2a
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-MyoD1 antibody [5.2F]
MyoD1 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [5.2F] to MyoD1 -
由来種
Mouse -
特異性
This antibody does not cross react with Myogenin, Myf5, or Myf6. -
アプリケーション
適用あり: Sandwich ELISA, IHC-Pmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Chicken, Pig
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免疫原
Synthetic peptide corresponding to Mouse MyoD1 aa 3-56 (N terminal).
Sequence:LLSPPLRDIDLTGPDGSLCSFETADDFYDDPCFDSPDLRFFEDLDPRLVH VGAL
Database link: P10085 -
ポジティブ･コントロール
- WB: Mouse muscle tissue lysate. ICC/IF: Rat differentiated skeletal muscle cells. IHC-P: Human rhabdomyosarcoma.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.08% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
モノクローナル -
クローン名
5.2F -
ミエローマ
Sp2/0-Ag14 -
アイソタイプ
IgG2a -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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sELISA pair antibody
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab16148の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Sandwich ELISA |
Use a concentration of 5 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to MyoD1 (ab64159). For sandwich ELISA, use this antibody as Capture at 5 µg/ml with Rabbit polyclonal to MyoD1 (ab64159) as Detection.
|
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| IHC-P | (6) |
Use a concentration of 2 - 4 µg/ml.
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| 特記事項 |
|---|
|
Sandwich ELISA
Use a concentration of 5 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to MyoD1 (ab64159). For sandwich ELISA, use this antibody as Capture at 5 µg/ml with Rabbit polyclonal to MyoD1 (ab64159) as Detection. |
|
IHC-P
Use a concentration of 2 - 4 µg/ml. |
ターゲット情報
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機能
Involved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Activates muscle-specific promoters. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins. -
配列類似性
Contains 1 basic helix-loop-helix (bHLH) domain. -
翻訳後修飾
Acetylated by a complex containing EP300 and PCAF. The acetylation is essential to activate target genes. Conversely, its deacetylation by SIRT1 inhibits its function.
Ubiquitinated on the N-terminus; which is required for proteasomal degradation. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 374048 Chicken
- Entrez Gene: 4654 Human
- Entrez Gene: 17927 Mouse
- Entrez Gene: 407604 Pig
- Omim: 159970 Human
- SwissProt: P16075 Chicken
- SwissProt: P15172 Human
- SwissProt: P10085 Mouse
see all -
別名
- bHLHc1 antibody
- Class C basic helix-loop-helix protein 1 antibody
- MYF 3 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MyoD1 antibody [5.2F] (ab16148)
Immunohistochemical staining using ab16148 at 2ug/ml on formalin fixed, paraffin embedded samples of human rhabdomyosarcoma.
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![Sandwich ELISA - Anti-MyoD1 antibody [5.2F] (ab16148)](https://www.abcam.co.jp/ps/products/16/ab16148/Images/ab16148-24868-anti-myod1-antibody-52f-sandwich-elisa.jpg)
Sandwich ELISA - Anti-MyoD1 antibody [5.2F] (ab16148)Standard Curve for Myo-D; dilution range 1 pg/ml to 1 ug/ml using Capture Antibody Mouse monoclonal [5.2F] to MyoD1 (ab16148) at 5ug/ml and Detector Antibody Rabbit polyclonal to MyoD1 (ab64159) at 0.1ug/ml.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (57)
ab16148 は 57 報の論文で使用されています。
- Bermejo-Álvarez I et al. Effects of Tofacitinib on Muscle Remodeling in Experimental Rheumatoid Sarcopenia. Int J Mol Sci 24:N/A (2023). PubMed: 37685986
- McNamara SL et al. Anti-inflammatory therapy enables robot-actuated regeneration of aged muscle. Sci Robot 8:eadd9369 (2023). PubMed: 36947599
- Wang M et al. Sulforaphane reduces lipopolysaccharide-induced inflammation and enhances myogenic differentiation of mouse embryonic myoblasts via the toll-like receptor 4 and NLRP3 pathways. Adv Clin Exp Med 32:457-467 (2023). PubMed: 36398371
- Cefis M et al. Endothelial cells are an important source of BDNF in rat skeletal muscle. Sci Rep 12:311 (2022). PubMed: 35013359
- Ji S et al. Myoblast-derived exosomes promote the repair and regeneration of injured skeletal muscle in mice. FEBS Open Bio 12:2213-2226 (2022). PubMed: 36325691