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AB16148

Anti-MyoD1 抗体 [5.2F]

Anti-MyoD1 antibody [5.2F]

3

(18 Reviews)

|

(68 Publications)

Mouse Monoclonal MYOD1 antibody. Suitable for sELISA, IHC-P and reacts with Mouse, Human samples. Cited in 68 publications. Immunogen corresponding to Synthetic Peptide within Mouse Myod1 aa 1-100.

別名を表示する

BHLHC1, MYF3, MYOD, MYOD1, Myoblast determination protein 1, Class C basic helix-loop-helix protein 1, Myogenic factor 3, bHLHc1, Myf-3

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MyoD1 antibody [5.2F] (AB16148)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MyoD1 antibody [5.2F] (AB16148)

Immunohistochemical staining using ab16148 at 2ug/ml on formalin fixed, paraffin embedded samples of human rhabdomyosarcoma.

Sandwich ELISA - Anti-MyoD1 antibody [5.2F] (AB16148)
  • sELISA

Unknown

Sandwich ELISA - Anti-MyoD1 antibody [5.2F] (AB16148)

Standard Curve for Myo-D; dilution range 1 pg/ml to 1 ug/ml using Capture Antibody Mouse monoclonal [5.2F] to MyoD1 (ab16148) at 5ug/ml and Detector Antibody Rabbit polyclonal to MyoD1 (ab64159) at 0.1ug/ml.

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

5.2F

アイソタイプ

IgG2a

キャリアフリー

No

交差種

Mouse, Human

アプリケーション

sELISA, IHC-P

applications

免疫原

Synthetic Peptide within Mouse Myod1 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

P10085

特異性

This antibody does not cross react with Myogenin, Myf5, or Myf6.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "2-4 µg/mL", "IHCP-species-notes": "<p></p>" }, "Mouse": { "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "5 µg/mL", "sELISA-species-notes": "<p>For sandwich ELISA, use this antibody as Capture at 5 µg/ml with Rabbit polyclonal to MyoD1 (<a href='/products/primary-antibodies/myod1-antibody-ab64159'>ab64159</a>) as Detection.</p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chicken": { "sELISA-species-checked": "predicted", "sELISA-species-dilution-info": "", "sELISA-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "sELISA-species-checked": "predicted", "sELISA-species-dilution-info": "", "sELISA-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Immunogen
バッファー組成
Preservative: 0.08% Sodium azide Constituents: PBS
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

MyoD1 also known as Myogenic Differentiation 1 is a master regulator of muscle differentiation. It functions mechanically as a transcription factor binding to DNA at specific sequences thereby activating the transcription of genes necessary for muscle tissue development. MyoD1 has a molecular weight of approximately 45 kDa. It is expressed in skeletal muscle tissues and also detected in some non-muscle tissues though at lower levels. MyoD1 is essential in the early stages of muscle cell lineage commitment.
Biological function summary

MyoD1 plays an important role in muscle differentiation by activating muscle-specific genes. It belongs to the myogenic regulatory factor family and often operates within a protein complex alongside Myf5 and myogenin aiding in the conversion of mesodermal stem cells into muscle cells. These partnerships enhance its ability to initiate the muscle-specific gene expression that drives myogenesis. MyoD1 acts like a molecular switch shifting cells into the pathway leading to muscle formation.

Pathways

MyoD1 is an integral component of myogenesis and muscle regeneration pathways. MyoD1 interacts with key pathway components such as Myf5 and MRF4 which reinforce MyoD1 activity in muscle tissue lineage determination and cell cycle arrest during differentiation. The activity of MyoD1 in these pathways highlights its role in skeletal muscle growth. MyoD1 also demonstrates functional redundancy with Myogenin as both share overlapping roles in these critical pathways.

MyoD1 is associated with muscular dystrophy and some sarcomas. Aberrant expression or mutations in MyoD1 can disrupt normal muscle differentiation contributing to muscle-related disorders like rhabdomyosarcoma a form of cancer comprising cells resembling skeletal muscle. Its relationship with other proteins like Myf5 further highlights its role in such pathological conditions as faults in these connections can amplify or alter disease progression.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation. Together with MYF5 and MYOG, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins (By similarity).
See full target information MYOD1
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文献 (68)

Recent publications for all applications. Explore the full list and refine your search

Veterinary sciences 12: PubMed41012786

2025

Effects of Dietary and Bacteriophage Supplementation on Water Quality, Carcass Traits, and Muscle Growth in Magang Geese.

Applications

Unspecified application

Species

Unspecified reactive species

Yong Li,Yongquan Luo,Yuanhao Han,Zhiyuan Liu,Songchao Li,Xiujin Li,Zhongping Wu,Yunbo Tian,Yunmao Huang,Xumeng Zhang

eLife 13: PubMed40960171

2025

A novel mouse model for -related muscular dystrophy with analysis of molecular pathogenesis and clinical phenotype.

Applications

Unspecified application

Species

Unspecified reactive species

Dandan Tan,Yidan Liu,Huaxia Luo,Qiang Shen,Xingbo Long,Luzheng Xu,Jieyu Liu,Nanbert A Zhong,Hong Zhang,Hui Xiong

The European journal of neuroscience 62:e70235 PubMed40960371

2025

Electrical Stimulation-Induced Muscle Damage Alters Hippocampal BDNF Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Julien Wirtz,Rémi Chaney,Marina Cefis,Alexandre Méloux,Yuan Wang,Stéphanie Lemaire,Aurore Quirié,Julien Delezie,Gilles Gouspillou,Anne Prigent-Tessier,Philippe Garnier

Frontiers in cell and developmental biology 13:1615676 PubMed40823533

2025

Integrated analysis of miRNAs-mRNAs in skeletal muscle development revealed that novel-miR-766 affects myoblast differentiation and myofiber-type formation in sheep.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenzhen Gu,WeiWei Duan,Chenxi Liu,Wenrong Li,Bin Han,Mingjun Liu

Veterinary medicine and science 10:e1412 PubMed38504633

2024

Polysaccharide of Atractylodes macrocephala Koidz alleviates LPS-induced proliferation, differentiation inhibition and excessive apoptosis in chicken embryonic myogenic cells.

Applications

Unspecified application

Species

Unspecified reactive species

Mengsi Fu,Jinhui Wang,Danning Xu,Nan Cao,Wanyan Li,Fada Li,Zhiyuan Liu,Yong Li,Chenyu Zhu,Yunmao Huang,Xumeng Zhang

International journal of molecular sciences 24: PubMed37685986

2023

Effects of Tofacitinib on Muscle Remodeling in Experimental Rheumatoid Sarcopenia.

Applications

Unspecified application

Species

Unspecified reactive species

Ismael Bermejo-Álvarez,Sandra Pérez-Baos,Paula Gratal,Juan Pablo Medina,Raquel Largo,Gabriel Herrero-Beaumont,Aránzazu Mediero

Science robotics 8:eadd9369 PubMed36947599

2023

Anti-inflammatory therapy enables robot-actuated regeneration of aged muscle.

Applications

Unspecified application

Species

Unspecified reactive species

S L McNamara,B R Seo,B R Freedman,E B Roloson,J T Alvarez,C T O'Neill,H H Vandenburgh,C J Walsh,D J Mooney

Life science alliance 6: PubMed36604149

2023

SMN promotes mitochondrial metabolic maturation during myogenesis by regulating the MYOD-miRNA axis.

Applications

Unspecified application

Species

Unspecified reactive species

Akihiro Ikenaka,Yohko Kitagawa,Michiko Yoshida,Chuang-Yu Lin,Akira Niwa,Tatsutoshi Nakahata,Megumu K Saito

Advances in clinical and experimental medicine : official organ Wroclaw Medical University 32:457-467 PubMed36398371

2022

Sulforaphane reduces lipopolysaccharide-induced inflammation and enhances myogenic differentiation of mouse embryonic myoblasts via the toll-like receptor 4 and NLRP3 pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Manfeng Wang,Meiling Liu,Wanzhen Xu,Zongyan Teng,Xiaowei Wu,Lu Gan,Yina Zhang

FEBS open bio 12:2213-2226 PubMed36325691

2022

Myoblast-derived exosomes promote the repair and regeneration of injured skeletal muscle in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Shusen Ji,Pei Ma,Xiaorui Cao,Juan Wang,Xiuju Yu,Xiaomao Luo,Jiayin Lu,Wei Hou,Zhijuan Zhang,Yi Yan,Yanjun Dong,Haidong Wang
View all publications
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代替製品

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Recombinant

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Primary Antibodies

AB209976

Anti-MyoD1 antibody

Lab Essentials

IHC - Wholemount - Anti-MyoD1 antibody (AB209976)

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Primary Antibodies

AB212662

Anti-MyoD1 antibody [5.8A] - BSA free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MyoD1 antibody [5.8A] - BSA free (AB212662)

  • 0
  • 1
Primary Antibodies

AB198251

Anti-MyoD1 antibody - C-terminal

Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MyoD1 antibody - C-terminal (AB198251)

  • 0
  • 0
Primary Antibodies

AB64159

Anti-MyoD1 antibody

Lab Essentials

Sandwich ELISA - Anti-MyoD1 antibody (AB64159)

  • 3
  • 3
Primary Antibodies

AB133627

Anti-MyoD1 antibody [EPR6653-131]

RabMAb|Recombinant|Lab Essentials|Advanced Validation|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MyoD1 antibody [EPR6653-131] (AB133627)

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Primary Antibodies

AB240073

Anti-MyoD1 antibody [EPR6653-131] - BSA and Azide free

Recombinant|Advanced Validation|RabMAb

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MyoD1 antibody [EPR6653-131] - BSA and Azide free (AB240073)

  • 0
  • 0
Primary Antibodies

AB203383

Anti-MyoD1 antibody

Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MyoD1 antibody (AB203383)

  • 3
  • 3

組合せ製品

Primary Antibodies

AB124800

Anti-Myogenin antibody [EPR4789]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myogenin antibody [EPR4789] (AB124800)

  • 5
  • 3
Primary Antibodies

AB37484

Anti-Myosin heavy chain antibody [A4.1025]

BOND RX™ Validated|Recombinant|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin heavy chain antibody [A4.1025] (AB37484)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB187339

Anti-PAX7 antibody

Immunocytochemistry/ Immunofluorescence - Anti-PAX7 antibody (AB187339)

  • 4
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