Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-MYL9 抗体 [EPR13013(2)(B)] (ab187152)

Submit a review Submit a question References (1)
Western blot - Anti-MYL9 antibody [EPR13013(2)(B)] (ab187152)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYL9 antibody [EPR13013(2)(B)] (ab187152)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYL9 antibody [EPR13013(2)(B)] (ab187152)
  • Western blot - Anti-MYL9 antibody [EPR13013(2)(B)] (ab187152)
  • Western blot - Anti-MYL9 antibody [EPR13013(2)(B)] (ab187152)
  • Anti-MYL9 antibody [EPR13013(2)(B)] (ab187152)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13013(2)(B)] to MYL9
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-MYL9 antibody [EPR13013(2)(B)]
    MYL9 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR13013(2)(B)] to MYL9

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-Pmore details

  • 種交差性

    交差種: Mouse, Human
    交差が予測される動物種: Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: HeLa and human colon cell lysate; Human stomach, colon and uterus lysates; Mouse muscle lysate. IHC-P: Human colon tissue; Mouse stomach tissue.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR13013(2)(B)

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab187152の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 20 kDa.
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 20 kDa.
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ターゲット情報

  • 機能

    Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion.

  • 組織特異性

    Smooth muscle tissues and in some, but not all, nonmuscle cells.

  • 配列類似性

    Contains 3 EF-hand domains.

  • 翻訳後修飾

    Phosphorylation increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. It is required to generate the driving force in the migration of the cells but not necessary for localization of myosin-2 at the leading edge.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • 20 kDa myosin light chain antibody
    • Human 20kDa myosin light chain (MLC2) mRNA complete cds antibody
    • LC20 antibody
    • MGC3505 antibody
    • MLC 2 antibody
    • MLC-2C antibody
    • MLC2 antibody
    • MLY 9 antibody
    • MRLC1 antibody
    • MYL9 antibody
    • MYL9_HUMAN antibody
    • Myosin light chain 9 regulatory antibody
    • Myosin light polypeptide 9 regulatory antibody
    • myosin regulatory light chain 1 antibody
    • Myosin regulatory light chain 2 antibody
    • Myosin regulatory light chain 2 smooth muscle isoform antibody
    • Myosin regulatory light chain 9 antibody
    • Myosin regulatory light chain MRLC1 antibody
    • Myosin regulatory light polypeptide 9 antibody
    • Myosin RLC antibody
    • Myosin vascular smooth muscle light chain 2 antibody
    • MYRL2 antibody
    • OTTHUMP00000030857 antibody
    • smooth muscle isoform antibody
    see all

画像

  • Western blot - Anti-MYL9 antibody [EPR13013(2)(B)] (ab187152)
    Western blot - Anti-MYL9 antibody [EPR13013(2)(B)] (ab187152)
    All lanes : Anti-MYL9 antibody [EPR13013(2)(B)] (ab187152) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : MYL9 knockout HeLa cell lysate
    Lane 3 : Human colon cell lysate
    Lane 4 : Daudi cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 20 kDa



    Lanes 1-4: Merged signal (red and green). Green - ab187152 observed at 20 kDa. Red - loading control ab8245 observed at 37 kDa.

     ab187152 Anti-MYL9 antibody [EPR13013(2)(B)] was shown to specifically react with MYL9 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab266036 (knockout cell lysate ab256999) was used. Wild-type and MYL9 knockout samples were subjected to SDS-PAGE. ab187152 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYL9 antibody [EPR13013(2)(B)] (ab187152)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYL9 antibody [EPR13013(2)(B)] (ab187152)

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue labeling MYL9 with ab187152 at 1/500 dilution. Anti-rabbit IgG HRP was used as a secondary antibody and slides were counterstained with hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYL9 antibody [EPR13013(2)(B)] (ab187152)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYL9 antibody [EPR13013(2)(B)] (ab187152)

    Immunohistochemical analysis of paraffin-embedded human colon tissue labeling MYL9 with ab187152 at 1/500 dilution. Anti-rabbit IgG HRP was used as a secondary antibody and slides were counterstained with hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Western blot - Anti-MYL9 antibody [EPR13013(2)(B)] (ab187152)
    Western blot - Anti-MYL9 antibody [EPR13013(2)(B)] (ab187152)
    Anti-MYL9 antibody [EPR13013(2)(B)] (ab187152) at 1/1000 dilution + Mouse muscle lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 20 kDa

  • Western blot - Anti-MYL9 antibody [EPR13013(2)(B)] (ab187152)
    Western blot - Anti-MYL9 antibody [EPR13013(2)(B)] (ab187152)
    All lanes : Anti-MYL9 antibody [EPR13013(2)(B)] (ab187152) at 1/5000 dilution

    Lane 1 : Human stomach lysate
    Lane 2 : Human colon lysate
    Lane 3 : Human uterus lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 20 kDa

  • Anti-MYL9 antibody [EPR13013(2)(B)] (ab187152)
    Anti-MYL9 antibody [EPR13013(2)(B)] (ab187152)

参考文献 (1)

ab187152 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab187152 は 1 報の論文で使用されています。

  • Fei L  et al. Systematic identification of cell-fate regulatory programs using a single-cell atlas of mouse development. Nat Genet 54:1051-1061 (2022). PubMed: 35817981

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

There are currently no Customer reviews or Questions for ab187152.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com