Anti-MYL9 抗体 (ab64161)
Key features and details
- Rabbit polyclonal to MYL9
- Suitable for: WB, IHC-P
- Reacts with: Human, Recombinant fragment
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-MYL9 antibody
MYL9 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to MYL9 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human, Recombinant fragment
交差が予測される動物種: Bird, Mammals
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免疫原
Synthetic peptide:
KKRPQRATSNVFAMFD
, corresponding to amino acids 12-27 of Human MYL9 -
ポジティブ･コントロール
- IHC-P: Human Smooth Muscle (colon).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab64161の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
1/500 - 1/2000. Predicted molecular weight: 20 kDa.
|
| IHC-P |
Use a concentration of 2.5 µg/ml.
|
| 特記事項 |
|---|
|
WB
1/500 - 1/2000. Predicted molecular weight: 20 kDa. |
|
IHC-P
Use a concentration of 2.5 µg/ml. |
ターゲット情報
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機能
Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion. -
組織特異性
Smooth muscle tissues and in some, but not all, nonmuscle cells. -
配列類似性
Contains 3 EF-hand domains. -
翻訳後修飾
Phosphorylation increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. It is required to generate the driving force in the migration of the cells but not necessary for localization of myosin-2 at the leading edge. - Information by UniProt
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参照データベース
- Entrez Gene: 10398 Human
- Omim: 609905 Human
- SwissProt: P24844 Human
- Unigene: 504687 Human
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別名
- 20 kDa myosin light chain antibody
- Human 20kDa myosin light chain (MLC2) mRNA complete cds antibody
- LC20 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYL9 antibody (ab64161)
ab64161 at 2.5µg/ml staining MYL9 in Colon, Human Smooth Muscle by Immunohistochemistry, Formalin-fixed, paraffin-embedded tissue.
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Western blot - Anti-MYL9 antibody (ab64161)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab64161 は 5 報の論文で使用されています。
- Zhu H et al. Identification of the hub genes associated with prostate cancer tumorigenesis. Front Oncol 13:1168772 (2023). PubMed: 37251946
- Zhu K et al. Long non-coding RNA MBNL1-AS1 regulates proliferation, migration, and invasion of cancer stem cells in colon cancer by interacting with MYL9 via sponging microRNA-412-3p. Clin Res Hepatol Gastroenterol N/A:N/A (2019). PubMed: 31255531
- Chao CM et al. Impact of Fgf10 deficiency on pulmonary vasculature formation in a mouse model of bronchopulmonary dysplasia. Hum Mol Genet 28:1429-1444 (2019). PubMed: 30566624
- Xie X & Percipalle P Elevated transforming growth factor ß signaling activation in ß-actin-knockout mouse embryonic fibroblasts enhances myofibroblast features. J Cell Physiol N/A:N/A (2018). PubMed: 29851084
- Hudson CA et al. Phasic contractions of isolated human myometrium are associated with Rho-kinase (ROCK)-dependent phosphorylation of myosin phosphatase-targeting subunit (MYPT1). Mol Hum Reprod 18:265-79 (2012). WB . PubMed: 22155728