Anti-MYL12A (phospho T17 + S18) 抗体 [EPR1457] (ab126739)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1457] to MYL12A (phospho T17 + S18)
- Suitable for: WB
- Reacts with: Mouse
Related conjugates and formulations
製品の概要
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製品名
Anti-MYL12A (phospho T17 + S18) antibody [EPR1457]
MYL12A 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR1457] to MYL12A (phospho T17 + S18) -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: Flow Cyt,ICC/IF,IHC-P or IP -
種交差性
交差種: Mouse
交差が予測される動物種: Rat, Human -
免疫原
Synthetic peptide corresponding to Human MYL12A (phospho T17 + S18).
Database link: P19105 -
ポジティブ・コントロール
- NIH/3T3 cell lysate.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR1457 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab126739の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/10000. Detects a band of approximately 18 kDa (predicted molecular weight: 20 kDa).
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特記事項 |
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WB
1/1000 - 1/10000. Detects a band of approximately 18 kDa (predicted molecular weight: 20 kDa). |
ターゲット情報
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機能
Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion. -
配列類似性
Contains 3 EF-hand domains. -
翻訳後修飾
Phosphorylation increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. It is required to generate the driving force in the migration of the cells but not necessary for localization of myosin-2 at the leading edge. - Information by UniProt
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参照データベース
- Entrez Gene: 10627 Human
- Entrez Gene: 67268 Mouse
- Entrez Gene: 501203 Rat
- SwissProt: P19105 Human
- SwissProt: D3YV37 Mouse
- Unigene: 190086 Human
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別名
- ML12A_HUMAN antibody
- MLC 2B antibody
- MLC-2B antibody
see all
画像
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All lanes : Anti-MYL12A (phospho T17 + S18) antibody [EPR1457] (ab126739) at 1/1000 dilution
Lane 1 : NIH/3T3 cell lysate
Lane 2 : NIH/3T3 cell lysate + Calyculin A
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat-anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 20 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab126739 は論文での使用が確認できていません。