Anti-MYL12A (phospho S19) 抗体 (ab2480)
Key features and details
- Rabbit polyclonal to MYL12A (phospho S19)
- Suitable for: ELISA, ICC/IF, IHC-FoFr, WB, IHC-P, IP
- Reacts with: Mouse, Rat, Human, Caenorhabditis elegans, Recombinant fragment
- Isotype: IgG
製品の概要
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製品名
Anti-MYL12A (phospho S19) antibody
MYL12A 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to MYL12A (phospho S19) -
由来種
Rabbit -
特異性
ab2480 can detect the phosphorylated serine residue in both the human and mouse protein. In human the phosphorylation site is pS19, while in mouse the site maps to pS20.
This phospho specific polyclonal antibody is specific for phosphorylated pS19 of human myosin light chain. Reactivity with non-phosphorylated human myosin light chain is less than 1% by ELISA. The selected peptide sequence used to generate the polyclonal antibody is located near the amino terminal end of the polypeptide corresponding to the smooth/non-muscle form of myosin regulatory light chain found in cardiac myocytes in addition to smooth and non-muscle cells. This sequence differs from that of the sarcomeric/cardiac form of myosin regulatory light chain that has a different sequence around the phosphorylation site. BLAST search analysis was used to determine that the smooth and non-muscle forms of myosin regulatory light chain have identical sequences. Cross reactivity is expected.
Customers have seen variable performance with Xenopus laevis.
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アプリケーション
適用あり: ELISA, ICC/IF, IHC-FoFr, WB, IHC-P, IPmore details -
種交差性
交差種: Mouse, Rat, Human, Caenorhabditis elegans, Recombinant fragment
交差が予測される動物種: Bird, Mammals -
免疫原
Synthetic peptide corresponding to Human MYL12A aa 1-100 (phospho S19) conjugated to keyhole limpet haemocyanin.
Database link: P19105 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 0.424% Tripotassium orthophosphate, 0.88% Sodium chloride -
Concentration information loading...
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精製度
Affinity purified -
特記事項(精製)
This affinity purified antibody is directed against the regulatory light chain of smooth and non-muscle myosin. The antibody is phosphospecific and detects monophosphorylated and diphosphorylated forms of the protein. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab2480の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ELISA |
1/10000 - 1/30000.
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ICC/IF | (6) |
Use at an assay dependent concentration.
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IHC-FoFr |
Use at an assay dependent concentration. PubMed: 19279134
Trichloroacetic acid was used for tissue fixation. |
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WB | (7) |
1/500 - 1/2000. Predicted molecular weight: 20 kDa.
We recommend using BSA for blocking when using phospho-specific antibodies. |
IHC-P | (4) |
Use a concentration of 2.5 µg/ml.
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IP |
Use at an assay dependent concentration.
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特記事項 |
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ELISA
1/10000 - 1/30000. |
ICC/IF
Use at an assay dependent concentration. |
IHC-FoFr
Use at an assay dependent concentration. PubMed: 19279134 Trichloroacetic acid was used for tissue fixation. |
WB
1/500 - 1/2000. Predicted molecular weight: 20 kDa. We recommend using BSA for blocking when using phospho-specific antibodies. |
IHC-P
Use a concentration of 2.5 µg/ml. |
IP
Use at an assay dependent concentration. |
ターゲット情報
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機能
Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion. -
配列類似性
Contains 3 EF-hand domains. -
翻訳後修飾
Phosphorylation increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. It is required to generate the driving force in the migration of the cells but not necessary for localization of myosin-2 at the leading edge. - Information by UniProt
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参照データベース
- Entrez Gene: 10627 Human
- Entrez Gene: 67268 Mouse
- Entrez Gene: 501203 Rat
- SwissProt: P19105 Human
- SwissProt: D3YV37 Mouse
- Unigene: 190086 Human
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別名
- ML12A_HUMAN antibody
- MLC 2B antibody
- MLC-2B antibody
see all
画像
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All lanes : Anti-MYL12A (phospho S19) antibody (ab2480) at 1/1000 dilution
Lane 1 : Regulatory Light Chain Non-Phospho recombinant protein, 50 ng
Lane 2 : Regulatory Light Chain Phospho recombinant protein, 50 ng
Lane 3 : Smooth Muscle Non-Phospho recombinant protein, 50 ng
Lane 4 : Smooth Muscle Phospho recombinant protein, 50 ng
Secondary
All lanes : Peroxidase rabbit secondary antibody at 1/40000 dilution
Predicted band size: 20 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYL12A (phospho S19) antibody (ab2480)
IHC-P of ab2480 at 2.5 µg/ml staining both vascular and myometrial smooth muscle cells of the human uterus. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
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Rabbit polyclonal to phospho Myosin Light Chain phospho Myosin Light Chain (Ser 20) (ab2480) used at a 1/5000 dilution to detect myosin light chain by Western blot. Either 13 ug (lanes 1-3) or 20 ug (lanes 4-7) of a mouse cardiac myocyte lysate was loaded on a 4-20% Criterion gel for SDS-PAGE. Samples were either mock-treated or CLA treated:
Lane 1 : untreated 45 min
Lane 2 : CLA 50 nm 45 min
Lane 3 : CLA 100 nm 45 min
Lane 4 : A21 untreated 45 min
Lane 5 : A22 A23187 5 min
Lane 6 : A23 A23187 15 min
Lane 7 : A24 A23187 60 min
After washing, a 1/5,000 dilution of anti-rabbit HRP (ab7090) was used as secondary.Rabbit polyclonal to phospho Myosin Light Chain phospho Myosin Light Chain (Ser 20) (ab2480) used at a 1/5000 dilution to detect myosin light chain by Western blot. Either 13 ug (lanes 1-3) or 20 ug (lanes 4-7) of a mouse cardiac myocyte lysate was loaded on a 4-20
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (144)
ab2480 は 144 報の論文で使用されています。
- Javary J et al. Phosphoproteomic analysis identifies supervillin as an ERK3 substrate regulating cytokinesis and cell ploidy. J Cell Physiol 239:e30938 (2024). PubMed: 36576983
- Fang X et al. Longitudinal characterization of cerebral hemodynamics in the TgF344-AD rat model of Alzheimer's disease. Geroscience 45:1471-1490 (2023). PubMed: 36933144
- Yang S et al. Internalization of apoptotic cells during efferocytosis requires Mertk-mediated calcium influx. Cell Death Dis 14:391 (2023). PubMed: 37391432
- Sterling NA et al. An entosis-like process induces mitotic disruption in Pals1 microcephaly pathogenesis. Nat Commun 14:82 (2023). PubMed: 36604424
- Saghebasl S et al. Sandwich-like electro-conductive polyurethane-based gelatin/soybean oil nanofibrous scaffolds with a targeted release of simvastatin for cardiac tissue engineering. J Biol Eng 17:42 (2023). PubMed: 37415188