Anti-MYL1 抗体 [EPR9934(B)] (ab151749)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9934(B)] to MYL1
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
-
製品名
Anti-MYL1 antibody [EPR9934(B)]
MYL1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR9934(B)] to MYL1 -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), WBmore details
適用なし: ICC/IF,IHC-P or IP -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide within Human MYL1 aa 50-150. The exact sequence is proprietary.
-
ポジティブ・コントロール
- Human skeletal muscle, HT1080 and Saos2 lysates; HT1080 cells
-
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20ºC. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
-
精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EPR9934(B) -
アイソタイプ
IgG
関連製品
-
Alternative Versions
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab151749の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB |
1/1000 - 1/10000. Predicted molecular weight: 21 kDa.
|
特記事項 |
---|
Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Predicted molecular weight: 21 kDa. |
ターゲット情報
-
機能
Regulatory light chain of myosin. Does not bind calcium. -
配列類似性
Contains 3 EF-hand domains. -
翻訳後修飾
Isoform MLC3 is acetylated at position 2. - Information by UniProt
-
参照データベース
- Entrez Gene: 4632 Human
- Entrez Gene: 4634 Human
- Entrez Gene: 17897 Mouse
- Entrez Gene: 17901 Mouse
- Entrez Gene: 24585 Rat
- Entrez Gene: 56781 Rat
- Omim: 160780 Human
- Omim: 160790 Human
see all -
別名
- A1 catalytic antibody
- Alkali myosin light chain 1 antibody
- MLC1/MLC3 antibody
see all
画像
-
All lanes : Anti-MYL1 antibody [EPR9934(B)] (ab151749) at 1/1000 dilution
Lane 1 : Human skeletal muscle lysate
Lane 2 : HT1080 lysate
Lane 3 : Saos2 lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 21 kDa -
Intracellular flow cytometric analysis of permeabilized HT1080 cells labeling MYL1 with ab151749 at 1/10 dilution (red), compared with a negative control rabbit IgG (green).
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (1)
ab151749 は 1 報の論文で使用されています。
- Li C et al. Analysis of myosin genes in HNSCC and identify MYL1 as a specific poor prognostic biomarker, promotes tumor metastasis and correlates with tumor immune infiltration in HNSCC. BMC Cancer 23:840 (2023). PubMed: 37679666