Name: Anti-Myeloperoxidase antibody [EPR20257]
Brand: RabMAb
SKU: ab208670
Rating: 5 (6 reviews)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR20257] (ab208670)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR20257] to Myeloperoxidase
Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
Reacts with: Mouse, Rat, Human

Alexa Fluor® 488 Biotin Carrier Free

製品名

Anti-Myeloperoxidase antibody [EPR20257]
Myeloperoxidase 一次抗体製品一覧
製品の詳細

Rabbit monoclonal [EPR20257] to Myeloperoxidase
由来種

Rabbit
特異性

This antibody is specific to Myeloperoxidase heavy chain.
アプリケーション

適用あり: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details
種交差性

交差種: Mouse, Rat, Human
免疫原

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
ポジティブ･コントロール
- WB: Human fetal spleen lysate; Rat and mouse spleen lysates; HL-60 whole cell lysate. IHC-P: Human spleen and stomach cancer tissues; Mouse and rat spleen tissues. ICC/IF: HL-60 cells. Flow Cyt (intra): Mouse PBMC and HL-60 cells.
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

製品の状態

Liquid
保存方法

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
バッファー

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
精製度

Protein A purified
ポリ/モノ

モノクローナル
クローン名

EPR20257
アイソタイプ

IgG
研究分野

The Abpromise guarantee

Abpromise保証は、次のテスト済みアプリケーションにおけるab208670の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション	Abreviews	特記事項
Flow Cyt (Intra)		1/500.
WB		1/1000. Detects a band of approximately 89, 59 kDa (predicted molecular weight: 83 kDa).
IHC-P	(3)	1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF		1/100.

特記事項
Flow Cyt (Intra) 1/500.
WB 1/1000. Detects a band of approximately 89, 59 kDa (predicted molecular weight: 83 kDa).
IHC-P 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/100.

機能

Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity.
関連疾患

Defects in MPO are the cause of myeloperoxidase deficiency (MPD) [MIM:254600]. MPD is an autosomal recessive defect that results in disseminated candidiasis.
配列類似性

Belongs to the peroxidase family. XPO subfamily.
細胞内局在

Lysosome.
Target information above from: UniProt accession P05164 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
参照データベース
- Entrez Gene: 4353 Human
- Entrez Gene: 17523 Mouse
- Entrez Gene: 303413 Rat
- Omim: 606989 Human
- SwissProt: P05164 Human
- SwissProt: P11247 Mouse
- Unigene: 458272 Human
- Unigene: 4668 Mouse
別名
- 84 kDa myeloperoxidase antibody
- 89 kDa myeloperoxidase antibody
- EC 1.11.1.7 antibody
- EC1.11.2.2 antibody
- fj80f04 antibody
- MPO antibody
- mpx antibody
- myeloid-specific peroxidase antibody
- Myeloperoxidase antibody
- Myeloperoxidase heavy chain antibody
- Myeloperoxidase light chain antibody
- PERM_HUMAN antibody
- wu:fj80f04 antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR20257] (ab208670)

Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling Myeloperoxidase with ab208670 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasmic staining on neutrophils of human spleen is observed [PMID: 19566938].

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-Myeloperoxidase antibody [EPR20257] (ab208670)

All lanes : Anti-Myeloperoxidase antibody [EPR20257] (ab208670) at 1/1000 dilution

Lane 1 : Human fetal spleen lysate
Lane 2 : Rat spleen lysate
Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 83 kDa
Observed band size: 59 kDa why is the actual band size different from the predicted?

Exposure time: 10 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature. PMID: 3029127 PMID: 2154223.

Negative control: HeLa PMID 12040446.
Immunocytochemistry/ Immunofluorescence - Anti-Myeloperoxidase antibody [EPR20257] (ab208670)

Immunofluorescent analysis of 100% methanol-fixed HL-60 (Human promyelocytic leukemia cell line) and HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Myeloperoxidase with ab208670 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Confocal image showing cytoplasmic staining on HL-60 cell line.

Negative control: HeLa (PMID: 12040446).

The nuclear counterstain is DAPI (blue).

Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594)) at 1/200 dilution (red).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR20257] (ab208670)

Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue labeling Myeloperoxidase with ab208670 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasmic staining on neutrophils of human stomach cancer is observed [PMID: 19566938].

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cytometry (Intracellular) - Anti-Myeloperoxidase antibody [EPR20257] (ab208670)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed HeLa cells (left panel) and HL-60cells (right panel)labeling Myeloperoxidase with ab208670 at 1/500 dilution, compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control ( (ab172730);black)andunlabelled control (cells without incubation with primary and secondary antibodies; blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody. Negative control: HeLa (PMID: 12040446).
Western blot - Anti-Myeloperoxidase antibody [EPR20257] (ab208670)

Anti-Myeloperoxidase antibody [EPR20257] (ab208670) at 1/5000 dilution + Mouse spleen lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 83 kDa
Observed band size: 59 kDa why is the actual band size different from the predicted?

Exposure time: 10 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature. PMID: 3029127 PMID: 2154223.
Western blot - Anti-Myeloperoxidase antibody [EPR20257] (ab208670)

Anti-Myeloperoxidase antibody [EPR20257] (ab208670) at 1/20000 dilution + HL-60 (Human promyelocytic leukemia cell line) whole cell lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 83 kDa
Observed band size: 39,59,89 kDa why is the actual band size different from the predicted?

Exposure time: 1 second

Blocking/Dilution buffer: 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature. PMID: 2154223 PMID: 8384653.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR20257] (ab208670)

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling Myeloperoxidase with ab208670 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasmic staining on neutrophils of mouse spleen is observed [PMID: 19566938].

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR20257] (ab208670)

Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling Myeloperoxidase with ab208670 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasmic staining on neutrophils of rat spleen is observed [PMID: 19566938].

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cytometry (Intracellular) - Anti-Myeloperoxidase antibody [EPR20257] (ab208670)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed Mouse PBMC cells labeling Myeloperoxidase with ab208670 at 1/500 dilution (right), compared with a rabbit monoclonal IgG isotype control (ab172730;left). Goat anti rabbit IgG (Alexa Fluor^® 488) at 1/2000 dilution was used as the secondary antibody. Mouse peripheral blood mononuclear cells stained intracellularly with ab208670 (Right) and isotype control (Left). Only monocytes and granulocytes (larger SSC population) result in positive signal while the lymphocyte population remains unchanged.
Anti-Myeloperoxidase antibody [EPR20257] (ab208670)

Flow cytometry protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Click here to view the general protocols

SDS download

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Datasheet download

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ab208670 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab208670 は 162 報の論文で使用されています。

Cai M et al. Fibroblast Growth Factor 21 Relieves Lipopolysaccharide-Induced Acute Lung Injury by Suppressing JAK2/STAT3 Signaling Pathway. Inflammation 47:209-226 (2024). PubMed: 37864659
Ono N et al. Functional role of IL-19 in a mouse model of L-arginine-induced pancreatitis and related lung injury. Exp Anim 73:175-185 (2024). PubMed: 38057085
Yan S et al. Inhibition of IEC-6 Cell Proliferation and the Mechanism of Ulcerative Colitis in C57BL/6 Mice by Dandelion Root Polysaccharides. Foods 12:N/A (2023). PubMed: 37893693
Su D et al. Spatiotemporal single-cell transcriptomic profiling reveals inflammatory cell states in a mouse model of diffuse alveolar damage. Exploration (Beijing) 3:20220171 (2023). PubMed: 37933384
Zhu H et al. Low-intensity pulsed ultrasound alleviates doxorubicin-induced cardiotoxicity via inhibition of S100a8/a9-mediated cardiac recruitment of neutrophils. Bioeng Transl Med 8:e10570 (2023). PubMed: 38023700

View all Publications for this product

レビューと Q&A

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レビューを送る質問を送る

1-6 of 6 Abreviews or Q&A

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Dog Tissue sections (spleen & colon)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Tris-EDTA, pH9

Permeabilization

Specification

spleen & colon

Blocking step

(agent) for 10 minute(s) · Concentration: 100% · Temperature: 21°C

Fixative

10% NBF

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Apr 26 2022

Application

Immunohistochemistry (Frozen sections)

Sample

Mouse Tissue sections (Spleen)

Permeabilization

Specification

Spleen

Blocking step

10% Normal Serum, 1% BSA in TBS as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C

Fixative

Methanol

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Brett Bell

Verified customer

投稿 Oct 20 2020

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Mouse Tissue sections (Liver)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0

Permeabilization

Specification

Liver

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Oct 01 2020

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Mouse Tissue sections (Connective tissue)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: citrate

Permeabilization

Yes - Triton

Specification

Connective tissue

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Tina Yuan

Verified customer

投稿 Jan 05 2020

Application

Immunohistochemistry (Frozen sections)

Sample

Mouse Tissue sections (connective tissue)

Permeabilization

Yes - Triton

Specification

connective tissue

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Tina Yuan

Verified customer

投稿 Jan 05 2020

Application

Immunocytochemistry/ Immunofluorescence

Sample

Mouse Cell (Purified neutrophils)

Permeabilization

Yes - 0.1% Triton

Specification

Purified neutrophils

Blocking step

BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Oct 11 2018

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製品の概要

製品名

製品の詳細

由来種

特異性

アプリケーション

種交差性

免疫原

ポジティブ･コントロール

特記事項

製品の特性

製品の状態

保存方法

バッファー

精製度

ポリ/モノ

クローン名

アイソタイプ

研究分野

関連製品

Alternative Versions

Assay kits

Compatible Secondaries

Isotype control

Related Products

アプリケーション

The Abpromise guarantee

ターゲット情報

機能

関連疾患

配列類似性

細胞内局在

参照データベース

別名

画像

プロトコール

データシートおよび資料

SDS download

Datasheet download

Certificate of Compliance

参考文献 (162)

レビューと Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Myeloperoxidase antibody [EPR20257]

Immunohistochemistry (Frozen sections) abreview for Anti-Myeloperoxidase antibody [EPR20257]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Myeloperoxidase antibody [EPR20257]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Myeloperoxidase antibody [EPR20257]

Immunohistochemistry (Frozen sections) abreview for Anti-Myeloperoxidase antibody [EPR20257]

Immunocytochemistry/ Immunofluorescence abreview for Anti-Myeloperoxidase antibody [EPR20257]