AB208670

Anti-Myeloperoxidase 抗体 [EPR20257]

Name: Anti-Myeloperoxidase 抗体 [EPR20257] (ab208670) | Abcam
Brand: Abcam Limited
SKU: AB208670
Price: 32000 JPY
Availability: InStock
Rating: 5 (7 reviews)

Anti-Myeloperoxidase antibody [EPR20257]

RabMAb
Recombinant
20ul selling size
詳細を見る

(7 Reviews)

(350 Publications)

Anti-Myeloperoxidase antibody [EPR20257] (ab208670) is a rabbit monoclonal antibody detecting Myeloperoxidase in Western Blot, Flow Cytometry (Intra), IHC-P, ICC/IF. Suitable for Human, Mouse, Rat.

- Biophysical QC for unrivalled batch-batch consistency
- Over 250 publications

Rat version of this clone also available as ab300650. Same clone, same staining pattern, different species antibody backbone.
Learn more about chimeric antibodies

別名を表示する

Myeloperoxidase, MPO

10 Images

Supplier Data

Western blot - Anti-Myeloperoxidase antibody [EPR20257] (AB208670)

Blocking/Dilution buffer : 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature. PMID : 3029127 PMID : 2154223.

Negative control : HeLa PMID 12040446.

All lanes:

Western blot - Anti-Myeloperoxidase antibody [EPR20257] (ab208670) at 1/1000 dilution

Lane 1:

Human fetal spleen lysate at 20 µg

Lane 2:

Rat spleen lysate at 20 µg

Lane 3:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 83 kDa

Observed band size: 59 kDa

false

Exposure time: 10s

Immunocytochemistry/ Immunofluorescence - Anti-Myeloperoxidase antibody [EPR20257] (AB208670)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Myeloperoxidase antibody [EPR20257] (AB208670)

Immunofluorescent analysis of 100% methanol-fixed HL-60 (Human promyelocytic leukemia cell line) and HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Myeloperoxidase with ab208670 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Confocal image showing cytoplasmic staining on HL-60 cell line.

Negative control : HeLa (PMID : 12040446).

The nuclear counterstain is DAPI (blue).

Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594)) at 1/200 dilution (red).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR20257] (AB208670)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR20257] (AB208670)

Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling Myeloperoxidase with ab208670 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasmic staining on neutrophils of human spleen is observed [PMID : 19566938].

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-Myeloperoxidase antibody [EPR20257] (AB208670)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Myeloperoxidase antibody [EPR20257] (AB208670)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed 90% methanol permeabilized HeLa cells (left panel) and HL-60cells (right panel) labeling Myeloperoxidase with ab208670 at 1/500 dilution, compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730; black) and unlabelled control (cells without incubation with primary and secondary antibodies; blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

Negative control : HeLa (PMID : 12040446).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR20257] (AB208670)

Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue labeling Myeloperoxidase with ab208670 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasmic staining on neutrophils of human stomach cancer is observed [PMID : 19566938].

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Myeloperoxidase antibody [EPR20257] (AB208670)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed 90% methanol permeabilized Mouse PBMC cells labeling Myeloperoxidase with ab208670 at 1/500 dilution (right), compared with a rabbit monoclonal IgG isotype control (ab172730; left). Goat anti rabbit IgG (Alexa Fluor^® 488) at 1/2000 dilution was used as the secondary antibody. Mouse peripheral blood mononuclear cells stained intracellularly with ab208670 (Right) and isotype control (Left). Only monocytes and granulocytes (larger SSC population) result in positive signal while the lymphocyte population remains unchanged.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR20257] (AB208670)

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling Myeloperoxidase with ab208670 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasmic staining on neutrophils of mouse spleen is observed [PMID : 19566938].

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR20257] (AB208670)

Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling Myeloperoxidase with ab208670 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasmic staining on neutrophils of rat spleen is observed [PMID : 19566938].

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-Myeloperoxidase antibody [EPR20257] (AB208670)

Blocking/Dilution buffer : 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature. PMID : 2154223 PMID : 8384653.

All lanes:

Western blot - Anti-Myeloperoxidase antibody [EPR20257] (ab208670) at 1/20000 dilution

All lanes:

HL-60 (Human promyelocytic leukemia cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 83 kDa

Observed band size: 39 kDa,59 kDa,89 kDa

false

Exposure time: 1s

Supplier Data

Western blot - Anti-Myeloperoxidase antibody [EPR20257] (AB208670)

Blocking/Dilution buffer : 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature. PMID : 3029127 PMID : 2154223.

All lanes:

Western blot - Anti-Myeloperoxidase antibody [EPR20257] (ab208670) at 1/5000 dilution

All lanes:

Mouse spleen lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 83 kDa

Observed band size: 59 kDa

false

Exposure time: 10s

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR20257

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Rat, Human

アプリケーション

ICC/IF, IHC-P, Flow Cyt (Intra), WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特異性

This antibody is specific to Myeloperoxidase heavy chain.

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Reactivity data

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製品の詳細

Anti-Myeloperoxidase antibody [EPR20257] (ab208670) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt (Intra), ICC/IF, IHC-P and WB.

Anti-Myeloperoxidase antibody [EPR20257] (ab208670) was first used in a scientific publication in 2018 and has been cited over 162 times in peer reviewed journals. It's performance in IHC and Western Blot in mouse samples is trusted by the scientific community.

Abcam's high quality manufacturing and validation processes ensure Anti-Myeloperoxidase antibody [EPR20257] (ab208670) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.

Anti-Myeloperoxidase antibody [EPR20257] (ab208670) has 5 independent reviews from customers.

Anti-Myeloperoxidase antibody [EPR20257] (ab208670) specifically detects Myeloperoxidase (UniProt ID: P05164; Molecular weight: 79kDa) and is sold in 100 µL and 1 mL selling sizes.

Conjugation-ready, carrier free format available for antibody clone EPR20257 - ab221847.

Antibody clone EPR20257 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor^® 488, Biotin (ab225474, ab322301).

- Recombinant format for unrivalled batch-batch consistency: no need for same-lot requests

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Conditional Ambient

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Myeloperoxidase also called MPO is an enzyme that plays a critical role in the body's immune response. This protein has a mass of approximately 150 kDa and exists as a dimer composed of heavy and light polypeptide chains. Myeloperoxidase is prominently expressed in neutrophils and monocytes which are types of white blood cells important for combating infections. The enzyme catalyzes the production of hypochlorous acid and other reactive substances by utilizing hydrogen peroxide and chloride ions. These reactive substances help in neutralizing pathogens during the immune response.

Biological function summary

The generation of reactive oxygen species by myeloperoxidase is essential for microbicidal activity. Myeloperoxidase functions as part of the antimicrobial system in the phagosome which is the intracellular compartment where pathogens are degraded. This enzyme works in conjunction with other components of the immune system such as NADPH oxidase. By generating hypochlorous acid MPO contributes to the oxidative burst a rapid release of reactive oxygen species during the response to pathogens.

Pathways

Myeloperoxidase integrates into the immune defense and inflammatory pathways. In particular it is associated with the neutrophil degranulation pathway where it releases its enzymatic contents to fight off microbes. MPO also interacts with proteins involved in oxidative stress processes such as superoxide dismutase which moderates levels of reactive oxygen species in cells. These interactions ensure balance in the immune response preventing excessive tissue damage during inflammation.

Dysregulated MPO activity can contribute to the development of diseases. For instance myeloperoxidase is linked with atherosclerosis a cardiovascular condition where inflammation and oxidative stress lead to plaque formation in the arteries. It also associates with vasculitis an autoimmune disorder causing inflammation of blood vessels. Both disorders can relate to the inflammatory pathways that involve MPO and proteins like C-reactive protein which serves as a marker of inflammation. Understanding MPO's role in these conditions is important for effective therapeutic interventions.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity (PubMed : 9922160). Mediates the proteolytic cleavage of alpha-1-microglobulin to form t-alpha-1-microglobulin, which potently inhibits oxidation of low-density lipoprotein particles and limits vascular damage (PubMed : 25698971).

See full target information MPO

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文献 (350)

Recent publications for all applications. Explore the full list and refine your search

Journal of translational medicine 23:1074 PubMed41074088

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Proteomic insights into platelet dysregulation and pathogenic mechanisms of chronic thromboembolic pulmonary hypertension.

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Nature communications 16:8870 PubMed41053178

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Paraventricular nucleus CRH neurons regulate acute lung injury via sympathetic nerve-neutrophil axis.

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Hui Li,Tao Liu,Yang Wang,Xue-Mei Miao,Yi-Yu Xiong,Qian Zhao,Wei-Yun Shen,Fu-Hong Su,Kang Chen,Ru-Ping Dai

Cell communication and signaling : CCS 23:416 PubMed41039446

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Off-target engagement of sotorasib with PPARγ via FABP4: a novel mechanism driving interstitial lung disease.

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Jiaqi Zhang,Jinjin Li,Mengting Cheng,Haiyang Zhou,Xiaochen Zhang,Hao Yan,Zhifei Xu,Bo Yang,Qiaojun He,Zizheng Gao,Xueqin Chen,Peihua Luo

iScience 28:113519 PubMed41035689

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NRF2 activation in cancer cells suppresses immune infiltration into the tumor microenvironment.

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Huaichun Wen,Takafumi Suzuki,Anqi Zhang,Miu Sato,Mahiro Matsumoto,Yuka Takahashi,Yushi Takahashi,Masayuki Yamamoto

The Journal of clinical investigation 135: PubMed41031886

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Pharmacological targeting of the IL-17/neutrophil axis attenuates calcific deposits in rat models of calciphylaxis.

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Bo Tao,Edward Z Cao,James Hyun,Sivakumar Ramadoss,Juan F Alvarez,Lianjiu Su,Qihao Sun,Zhihao Liu,Linlin Zhang,Alejandro Espinoza,Yiqian Gu,Feiyang Ma,Shen Li,Matteo Pellegrini,Arjun Deb

Journal of nanobiotechnology 23:612 PubMed41023976

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Lactobacillus johnsonii-derived extracellular vesicles restore mucosal immunity via taurine-linked Th17/Treg and IgA/IgG regulation in colitis.

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Hailan Zhao,Ningning Yue,Zhiliang Mai,Yuan Zhang,Chengmei Tian,Chen Kong,Longbin Huang,Ruiyue Shi,Yujie Liang,Jun Yao,Yuqiang Nie,Defeng Li,Biao Nie,Lisheng Wang

Molecular medicine (Cambridge, Mass.) 31:304 PubMed41023796

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NET degradation attenuates ricin-induced acute lung injury and protects mice from ARDS.

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Anita Sapoznikov,Yentl Evgy,Liat Fux,Ilya Ruderfer,Yakir Nataf,Yael Hayon,Shay Zamin,Roey Mizrachi,Rachel Pessah,Yoav Gal,Alon Ben-David,Noam Erez,Reut Falach

Stem cell research & therapy 16:507 PubMed41013769

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E2 pretreatment alleviates aggregation of intravenously injected mesenchymal stem cells in TBI by regulating BRG1 to affect adhesion.

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Wangan Li,Xiangyu Wang,Zhiming Feng,Shiting Hua,Xiaoxiong Zou,Zelin Lai,Yingqian Cai,Yuxi Zou,Yanping Tang,Cai Lin,Guobiao Luo,Xiaolin Cai,Xiaodan Jiang

Histology and histopathology :18990 PubMed40977347

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Penning decoction ameliorated pyroptosis in mice with lipopolysaccharide-induced endometritis through inhibition of the TLR4/NF-κB/NLRP3 pathway.

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Jiani Shi,Chen Chen,Yuqiong Yuan,Zhihui Liu,Qianru Zhou

Laboratory animal research 41:21 PubMed40958130

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Anti-allergic and anti-inflammatory effects of Gastrodia elata Blume extract in ovalbumin-induced asthma rat model.

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Jeong Su Park,Yeon Su Lee,Da Eun Jung,Ji Won Seo,Hyeon Jeong Na,Jin Woo Hong,Jae-Ho Shin

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Myeloperoxidase 抗体 [EPR20257]

Anti-Myeloperoxidase antibody [EPR20257]

Western blot - Anti-Myeloperoxidase antibody [EPR20257] (AB208670)

Immunocytochemistry/ Immunofluorescence - Anti-Myeloperoxidase antibody [EPR20257] (AB208670)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR20257] (AB208670)

Flow Cytometry (Intracellular) - Anti-Myeloperoxidase antibody [EPR20257] (AB208670)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR20257] (AB208670)

Flow Cytometry (Intracellular) - Anti-Myeloperoxidase antibody [EPR20257] (AB208670)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR20257] (AB208670)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR20257] (AB208670)

Western blot - Anti-Myeloperoxidase antibody [EPR20257] (AB208670)

Western blot - Anti-Myeloperoxidase antibody [EPR20257] (AB208670)

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Key facts

宿主種

クローン性

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アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

特異性

代替製品

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Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (350)

Abcam product promise