Anti-Myeloperoxidase 抗体 (ab45977)
Key features and details
- Rabbit polyclonal to Myeloperoxidase
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Myeloperoxidase antibody
Myeloperoxidase 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Myeloperoxidase -
由来種
Rabbit -
特異性
ab45977 has been batch tested using human lysates/tissues only. Some customers have successfully used ab45977 with rat. Please contact Abcam Scientific Support for more information. -
アプリケーション
適用あり: ICC/IF, WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Rat, Common marmoset
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免疫原
Synthetic peptide corresponding to Human Myeloperoxidase aa 150-250 conjugated to keyhole limpet haemocyanin.
(Peptide available asab45976) -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Assay kits
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab45977の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | (1) |
Use a concentration of 5 - 10 µg/ml.
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| WB | (2) |
Use a concentration of 1 µg/ml. Detects a band of approximately 84 kDa (predicted molecular weight: 84 kDa).
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| IHC-P | (11) |
Use a concentration of 1 µg/ml.
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| 特記事項 |
|---|
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ICC/IF
Use a concentration of 5 - 10 µg/ml. |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 84 kDa (predicted molecular weight: 84 kDa). |
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IHC-P
Use a concentration of 1 µg/ml. |
ターゲット情報
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機能
Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity. -
関連疾患
Defects in MPO are the cause of myeloperoxidase deficiency (MPD) [MIM:254600]. MPD is an autosomal recessive defect that results in disseminated candidiasis. -
配列類似性
Belongs to the peroxidase family. XPO subfamily. -
細胞内局在
Lysosome. - Information by UniProt
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参照データベース
- Entrez Gene: 4353 Human
- Entrez Gene: 303413 Rat
- Omim: 606989 Human
- SwissProt: P05164 Human
- Unigene: 458272 Human
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別名
- 84 kDa myeloperoxidase antibody
- 89 kDa myeloperoxidase antibody
- EC 1.11.1.7 antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-Myeloperoxidase antibody (ab45977)
ab45977 stained in HL60 cells. Cells were fixed with 100% methanol (5min) at room temperature and incubated with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 1h at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab45977 at 5µg/ml and ab72791 (Mouse monoclonal to alpha Tubulin - Loading Control) at 1ug/ml overnight at +4°C. The secondary antibodies used were ab150081 used at 1 ug/ml (colored green) and ab150116 (pseudo-colored red) used at 2ug/ml for 1hour at room temperature. DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43µM for 1hour at room temperature.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody (ab45977)This image is courtesy of an Abreview submitted by Antibody Solutions Ltd.ab45977 (1/50) staining Myeloperoxidase in paraffin-embbeded human spleen tissue sections. Tissue underwent fixation in formaldehyde, heat-mediated antigen retrieval in citrate buffer pH 6.0 and blocking (5 minutes/peroxidase block and 10 minutes/protein block). For further experimental details please refer to abreview. -
Western blot - Anti-Myeloperoxidase antibody (ab45977)Anti-Myeloperoxidase antibody (ab45977) at 1 µg/ml + HL60 (Human promyelocytic leukemia cell line) Whole Cell Lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 84 kDa
Observed band size: 84 kDa
Exposure time: 2 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab45977 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody (ab45977)IHC image of 45977 staining in human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab45977, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. -
Western blot - Anti-Myeloperoxidase antibody (ab45977)Anti-Myeloperoxidase antibody (ab45977) at 1 µg/ml + HL60 (Human promyelocytic leukemia cell line) Whole Cell Lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Predicted band size: 84 kDa
Observed band size: 84 kDa
Additional bands at: 12 kDa, 35 kDa, 75 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutesThe mature myeloperoxidase protein is a tetramer of two heavy chains (60 kDa) and two light chains (12 kDa). Our immunogen sequence is within the myeloperoxidase light chain. In HL60 cells, ab45977 detects bands at approximately 84-kDa, corresponding to the expected MW of full-length Myeloperoxidase protein, and at 12-kDa, corresponding to the expected MW of myeloperoxidase light chain.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (78)
ab45977 は 78 報の論文で使用されています。
- Lavie L et al. Giant phagocytes (Gφ) and neutrophil-macrophage hybrids in human carotid atherosclerotic plaques - An activated phenotype. Front Immunol 14:1101569 (2023). PubMed: 36911715
- Zhao J et al. NETs Promote Inflammatory Injury by Activating cGAS-STING Pathway in Acute Lung Injury. Int J Mol Sci 24:N/A (2023). PubMed: 36982193
- Liu Y et al. Neutrophil extracellular traps predict postoperative pulmonary complications in paediatric patients undergoing parental liver transplantation. BMC Gastroenterol 23:237 (2023). PubMed: 37442949
- Yao L et al. Imbalance of Innate and Adaptive Immunity in Esophageal Achalasia. J Neurogastroenterol Motil 29:486-500 (2023). PubMed: 37586778
- Kawasaki M et al. Neutrophil degranulation interconnects over-represented biological processes in atrial fibrillation. Sci Rep 11:2972 (2021). PubMed: 33536523