AB25989

Anti-Myeloperoxidase 抗体 [2C7]

Name: Anti-Myeloperoxidase 抗体 [2C7] (ab25989) | Abcam
Brand: Abcam Limited
SKU: AB25989
Price: 69000 JPY
Availability: InStock
Rating: 3 (6 reviews)

Anti-Myeloperoxidase antibody [2C7]

(6 Reviews)

(111 Publications)

Anti-Myeloperoxidase antibody [2C7] (ab25989) is a mouse monoclonal antibody detecting Myeloperoxidase in Flow Cytometry. Suitable for Human.

- Over 90 publications
- Trusted since 2005

別名を表示する

Myeloperoxidase, MPO

1 Images

Flow Cytometry (Intracellular) - Anti-Myeloperoxidase antibody [2C7] (AB25989)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-Myeloperoxidase antibody [2C7] (AB25989)

Flow cytometry overlay histogram showing left HL-60 positive cells and right negative HeLa stained with ab25989 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilised with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS containing 10μg/ml human IgG and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab25989) (1x 10⁶ in 100μl at 0.04μg/ml (1/25000)) for 30min at 22°C.

The secondary antibody Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed was incubated at 1/4000 for 30min at 22°C

Isotype control antibody (black line) was Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.

This antibody gave a positive signal in HL-60 Fixed with 80% methanol (5 min) / permeabilised with 0.1% PBS-Triton X-100 for 15 min under the same conditions.

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

2C7

アイソタイプ

IgG1

キャリアフリー

交差種

Human

アプリケーション

Flow Cyt

applications

免疫原

Native Full Length Protein corresponding to Human MPO.

P05164

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/25000", "FlowCyt-species-notes": "<p></p>" } } }

style

left-column

製品の詳細

What is this antibody validated in?
Anti-Myeloperoxidase antibody [2C7] (ab25989) is a mouse monoclonal antibody and is validated for use in Flow Cytometry (Flow Cyt) in Human samples.

Trusted by the scientific community
Anti-Myeloperoxidase [2C7] (ab25989) was first used in a scientific publication in 2005 and has been cited over 90 times in peer-reviewed journals.

Reviewed by scientists
Anti-Myeloperoxidase [2C7] (ab25989) has over 5 independent reviews from customers.

Other related products
We have a range of other formats of antibody clone [2C7] also available for your convenience: ab283263, Carrier free - ab283284, FITC - ab11729, PE - ab11730

style

left-column

出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

Preservative: 0.09% Sodium azide Constituents: PBS

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

style

left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Myeloperoxidase also called MPO is an enzyme that plays a critical role in the body's immune response. This protein has a mass of approximately 150 kDa and exists as a dimer composed of heavy and light polypeptide chains. Myeloperoxidase is prominently expressed in neutrophils and monocytes which are types of white blood cells important for combating infections. The enzyme catalyzes the production of hypochlorous acid and other reactive substances by utilizing hydrogen peroxide and chloride ions. These reactive substances help in neutralizing pathogens during the immune response.

Biological function summary

The generation of reactive oxygen species by myeloperoxidase is essential for microbicidal activity. Myeloperoxidase functions as part of the antimicrobial system in the phagosome which is the intracellular compartment where pathogens are degraded. This enzyme works in conjunction with other components of the immune system such as NADPH oxidase. By generating hypochlorous acid MPO contributes to the oxidative burst a rapid release of reactive oxygen species during the response to pathogens.

Pathways

Myeloperoxidase integrates into the immune defense and inflammatory pathways. In particular it is associated with the neutrophil degranulation pathway where it releases its enzymatic contents to fight off microbes. MPO also interacts with proteins involved in oxidative stress processes such as superoxide dismutase which moderates levels of reactive oxygen species in cells. These interactions ensure balance in the immune response preventing excessive tissue damage during inflammation.

Dysregulated MPO activity can contribute to the development of diseases. For instance myeloperoxidase is linked with atherosclerosis a cardiovascular condition where inflammation and oxidative stress lead to plaque formation in the arteries. It also associates with vasculitis an autoimmune disorder causing inflammation of blood vessels. Both disorders can relate to the inflammatory pathways that involve MPO and proteins like C-reactive protein which serves as a marker of inflammation. Understanding MPO's role in these conditions is important for effective therapeutic interventions.

style

left-column

style

left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

ターゲットの情報

Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity (PubMed : 9922160). Mediates the proteolytic cleavage of alpha-1-microglobulin to form t-alpha-1-microglobulin, which potently inhibits oxidation of low-density lipoprotein particles and limits vascular damage (PubMed : 25698971).

See full target information MPO

style

left-column

文献 (111)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 12:e11873 PubMed40832885

2025

Succinate Dehydrogenase Subunit A (SDHA) Mediated Microglia Extracellular Traps Formation Participating in Cerebral Ischemic Reperfusion Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Lili Zhao,Tao Li,Meijuan Dang,Ye Li,Jialiang Lu,Ziwei Lu,Zhiyang Chen,Qiao Huang,Yujie Chen,Yang Yang,Yuxuan Feng,Xiaoya Wang,Yating Jian,Heying Wang,Yingying Guo,Lei Zhang,Yu Jiang,Songhua Fan,Shengxi Wu,Hong Fan,Fang Kuang,Guilian Zhang

Lupus science & medicine 12: PubMed40750120

2025

Endotoxaemia in childhood-onset systemic lupus erythematosus induces low-density granulocytes and extracellular traps of innate immune cells.

Applications

Unspecified application

Species

Unspecified reactive species

Wilasinee Saisorn,Pornpimol Phuengmaung,Chanunya Santiworakul,Kent Doi,Pornpimol Rianthavorn,Asada Leelahavanichkul

ACS applied materials & interfaces 17:8862-8874 PubMed39884672

2025

Physicochemical Design of Nanoparticles to Interface with and Degrade Neutrophil Extracellular Traps.

Applications

Unspecified application

Species

Unspecified reactive species

Preethi Raghavan,Cynthia A Perez,Thomas A Sorrentino,Jacqueline C Kading,Joel A Finbloom,Tejal A Desai

Frontiers in immunology 15:1436926 PubMed39315100

2024

Neutrophil extracellular traps and citrullinated fibrinogen contribute to injury in a porcine model of limb ischemia and reperfusion.

Applications

Unspecified application

Species

Unspecified reactive species

Valentina Zollet,Isabel Arenas Hoyos,Stefanie Hirsiger,Bilal Ben Brahim,Maria Francesca Petrucci,Daniela Casoni,Junhua Wang,Rolf Spirig,Kay Nettelbeck,Luisana Garcia,Lena Fuest,Esther Vögelin,Mihai Constantinescu,Robert Rieben

International journal of molecular sciences 25: PubMed39273570

2024

Complement-Mediated Two-Step NETosis: Serum-Induced Complement Activation and Calcium Influx Generate NADPH Oxidase-Dependent NETs in Serum-Free Conditions.

Applications

Unspecified application

Species

Unspecified reactive species

Maria Maqsood,Samuel Suntharalingham,Meraj Khan,Carolina G Ortiz-Sandoval,Wouter J C Feitz,Nades Palaniyar,Christoph Licht

Cell reports 43:114493 PubMed39028622

2024

Mitochondrial perturbations in low-protein-diet-fed mice are associated with altered neutrophil development and effector functions.

Applications

Unspecified application

Species

Unspecified reactive species

Mehakpreet K Thind,Emiliano Miraglia,Catriona Ling,Meraj A Khan,Aida Glembocki,Celine Bourdon,YueYing ChenMi,Nades Palaniyar,Michael Glogauer,Robert H J Bandsma,Amber Farooqui

Frontiers in immunology 15:1387945 PubMed38887281

2024

A local drug delivery system prolongs graft survival by dampening T cell infiltration and neutrophil extracellular trap formation in vascularized composite allografts.

Applications

Unspecified application

Species

Unspecified reactive species

Isabel Arenas Hoyos,Anja Helmer,Anaïs Yerly,Ioana Lese,Stefanie Hirsiger,Lei Zhang,Daniela Casoni,Luisana Garcia,MariaFrancesca Petrucci,Sabine E Hammer,Tereza Duckova,Yara Banz,Matteo Montani,Mihai Constantinescu,Esther Vögelin,Gregor Bordon,Simone Aleandri,Jean-Christophe Prost,Adriano Taddeo,Paola Luciani,Robert Rieben,Nicoletta Sorvillo,Radu Olariu

Rheumatology (Oxford, England) 63:2578-2589 PubMed38837706

2024

FTY720 ameliorates experimental MPO-ANCA-associated vasculitis by regulating fatty acid oxidation via the neutrophil PPARα-CPT1a pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Rui-Xue Wang,Luo-Yi Wang,Xiang-Yu Han,Su-Fang Chen,Xiao-Jing Sun,Zhi-Ying Li,Mark A Little,Ming-Hui Zhao,Min Chen

Cancers 16: PubMed38730718

2024

Susceptibility of Melanoma Cells to Targeted Therapy Correlates with Protection by Blood Neutrophils.

Applications

Unspecified application

Species

Unspecified reactive species

Simone Wendlinger,Jonas Wohlfarth,Claudia Siedel,Sophia Kreft,Teresa Kilian,Sarah Junker,Luisa Schmid,Tobias Sinnberg,Ulrich Dischinger,Markus V Heppt,Kilian Wistuba-Hamprecht,Friedegund Meier,Luise Erpenbeck,Elsa Neubert,Matthias Goebeler,Anja Gesierich,David Schrama,Corinna Kosnopfel,Bastian Schilling

Nature methods 21:868-881 PubMed38374263

2024

Generation of complex bone marrow organoids from human induced pluripotent stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Stephanie Frenz-Wiessner,Savannah D Fairley,Maximilian Buser,Isabel Goek,Kirill Salewskij,Gustav Jonsson,David Illig,Benedicta Zu Putlitz,Daniel Petersheim,Yue Li,Pin-Hsuan Chen,Martina Kalauz,Raffaele Conca,Michael Sterr,Johanna Geuder,Yoko Mizoguchi,Remco T A Megens,Monika I Linder,Daniel Kotlarz,Martina Rudelius,Josef M Penninger,Carsten Marr,Christoph Klein

View all publications

style

left-column

style

left-column

style

right-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Myeloperoxidase 抗体 [2C7]

Anti-Myeloperoxidase antibody [2C7]

Flow Cytometry (Intracellular) - Anti-Myeloperoxidase antibody [2C7] (AB25989)

関連する標識済み抗体及び組成の異なる製品 (2)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (111)

Abcam product promise