Anti-Myelin oligodendrocyte glycoprotein 抗体 [EP4281]

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] - Oligodendrocyte Marker (AB109746)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebrum tissue sections labeling Myelin oligodendrocyte glycoprotein with purified ab109746 at 1 : 1000 dilution (1.12 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use). PBS instead of the primary antibody was used as the negative control.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] - Oligodendrocyte Marker (AB109746)

ab109746 showing negative staining in Normal colon tissue. This image was produced using unpurified antibody.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] - Oligodendrocyte Marker (AB109746)

ab109746 showing negative staining in Breast carcinoma tissue. This image was produced using unpurified antibody.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] - Oligodendrocyte Marker (AB109746)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human astrocytoma tissue sections labeling Myelin oligodendrocyte glycoprotein with purified ab109746 at 1 : 1000 dilution (1.12 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use). PBS instead of the primary antibody was used as the negative control.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] - Oligodendrocyte Marker (AB109746)

ab109746 showing negative staining in Normal breast tissue. This image was produced using unpurified antibody.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] - Oligodendrocyte Marker (AB109746)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat cerebrum tissue sections labeling Myelin oligodendrocyte glycoprotein with purified ab109746 at 1 : 1000 dilution (1.12 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use). PBS instead of the primary antibody was used as the negative control.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] - Oligodendrocyte Marker (AB109746)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cerebrum tissue sections labeling Myelin oligodendrocyte glycoprotein with Purified ab109746 at 1 : 1000 dilution (1.12 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1 : 0 dilution. PBS instead of the primary antibody was used as the negative control.

• WB

Lab

Western blot - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] - Oligodendrocyte Marker (AB109746)

Blocking buffer concentrations : 5% NFDM/TBST

Diluting buffer concentrations : 5% NFDM/TBST

Exposure equipment : iBright CL 1000 Imaging System

All lanes:

Western blot - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] - Oligodendrocyte Marker (ab109746) at 1/1000 dilution

All lanes:

Human brain tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 28 kDa

Observed band size: 25 kDa,15 kDa

true

Exposure time: 3s

• WB

Lab

Western blot - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] - Oligodendrocyte Marker (AB109746)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The bands beneath the target band (25 kDa) are likely to be degraded target fragments.

In lanes 1-2, to minimize protein degradation, cells were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

In lanes 3-4, this blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

Exposure time :

Lanes 1-2 : 180 seconds,

Lanes 3-4 : 92 seconds.

Exposure equipment : iBright CL 1000 Imaging System.

All lanes:

Western blot - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] - Oligodendrocyte Marker (ab109746) at 1/1000 dilution

Lanes 1 and 3:

Mouse brain tissue lysate at 20 µg

Lanes 2 and 4:

Rat brain tissue lysate at 20 µg

Secondary

Lanes 1 - 2:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Lanes 3 - 4:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 28 kDa

Observed band size: 25 kDa,15 kDa

true

• WB

Lab

Western blot - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] - Oligodendrocyte Marker (AB109746)

Exposure time : Lane 1-3&5-7 : 20 seconds; Lane 4&8 : 180 seconds

In Western blot analysis, ab109746 exhibited lower sensitivity compared to ab108505 and ab233549, for which reason we recommend the latter two as superior alternatives

Protocol optimizations including increased protein loading (30-50 μg/lane), reduced primary antibody dilution (1 : 500), and fg-grade ECL substrates are suggested if ab109746 must be used.

Lanes 1, 4, 5 and 8:

Western blot - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] - Oligodendrocyte Marker (ab109746) at 1/1000 dilution

Lanes 2 and 6:

Western blot - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR4282] - Oligodendrocyte Marker (<a href='/products/primary-antibodies/myelin-oligodendrocyte-glycoprotein-antibody-epr4282-oligodendrocyte-marker-ab108505'>ab108505</a>) at 1/1000 dilution

Lanes 3 and 7:

Western blot - Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] - Oligodendrocyte Marker (<a href='/products/primary-antibodies/myelin-oligodendrocyte-glycoprotein-antibody-epr22629-310-oligodendrocyte-marker-ab233549'>ab233549</a>) at 1/1000 dilution

Lanes 1 - 4:

Mouse brain tissue lysate at 20 µg

Lanes 5 - 8:

Rat brain tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 25 kDa

false

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] - Oligodendrocyte Marker (AB109746)

ab109746 showing negative staining in Benign prostatic hyperplasia tissue. This image was produced using unpurified antibody.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] - Oligodendrocyte Marker (AB109746)

ab109746 showing negative staining in Colonic adenocarcinoma tissue. This image was produced using unpurified antibody.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] - Oligodendrocyte Marker (AB109746)

ab109746 showing negative staining in Normal tonsil tissue. This image was produced using unpurified antibody.

• WB

CiteAb

Western blot - Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] - Oligodendrocyte Marker (AB109746)

Myelin oligodendrocyte glycoprotein western blot using anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] ab109746. Publication image and figure legend from Hoban, A. E., Stilling, R. M., et al., 2016, Transl Psychiatry, PubMed 27045844.

ab109746 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab109746 please see the product overview.

Increased myelin protein in male germ-free (GF) and exGF mice. (a) Western blot analysis for MOG in the prefrontal cortex (PFC) of conventional (CON), GF and exGF. (b) Quantification of protein concentration was normalized to β-III-tubulin and expressed relative to control levels. (c) Schematic representation of findings highlighting the transcriptional network driving increased myelination. Bar graph data is shown as mean±s.e.m. *p<0.05; **p<0.01.

false