Anti-Myelin oligodendrocyte glycoprotein 抗体 (ab32760)
Key features and details
- Rabbit polyclonal to Myelin oligodendrocyte glycoprotein
- Suitable for: WB, IHC-FoFr, IP
- Reacts with: Mouse, Rat
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Myelin oligodendrocyte glycoprotein antibody
Myelin oligodendrocyte glycoprotein 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Myelin oligodendrocyte glycoprotein -
由来種
Rabbit -
特異性
Replenishment batches of our polyclonal antibody, ab32760 are tested in WB. Previous batches were additionally validated in IHC-FoFr and IP. These applications are still expected to work and are covered by our Abpromise guarantee. You may also be interested in our alternative recombinant antibody, ab233549.
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アプリケーション
適用あり: WB, IHC-FoFr, IPmore details -
種交差性
交差種: Mouse, Rat
交差が予測される動物種: Cow, Human, Common marmoset
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免疫原
Synthetic peptide corresponding to Rat Myelin oligodendrocyte glycoprotein aa 200 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab32759) -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab32760の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).
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| IHC-FoFr | (1) |
Use at an assay dependent concentration.
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| IP |
Use a concentration of 5 µg/ml.
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| 特記事項 |
|---|
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa). |
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IHC-FoFr
Use at an assay dependent concentration. |
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IP
Use a concentration of 5 µg/ml. |
ターゲット情報
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機能
Mediates homophilic cell-cell adhesion (By similarity). Minor component of the myelin sheath. May be involved in completion and/or maintenance of the myelin sheath and in cell-cell communication. -
組織特異性
Found exclusively in the CNS, where it is localized on the surface of myelin and oligodendrocyte cytoplasmic membranes. -
配列類似性
Belongs to the immunoglobulin superfamily. BTN/MOG family.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 100400400 Common marmoset
- Entrez Gene: 280863 Cow
- Entrez Gene: 4340 Human
- Entrez Gene: 17441 Mouse
- Entrez Gene: 24558 Rat
- Omim: 159465 Human
- SwissProt: Q29ZQ1 Common marmoset
- SwissProt: P55803 Cow
see all -
別名
- BTN6 antibody
- BTNL11 antibody
- MGC26137 antibody
see all
画像
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Western blot - Anti-Myelin oligodendrocyte glycoprotein antibody (ab32760)All lanes : Anti-Myelin oligodendrocyte glycoprotein antibody (ab32760) at 1 µg/ml
Lane 1 : Mouse Brain Whole Tissue Lysate
Lane 2 : Rat Brain Whole Tissue Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : IR Dye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size: 27 kDa
Observed band size: 27 kDa -
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Myelin oligodendrocyte glycoprotein antibody (ab32760)Image courtesy of Abreview from Sophie PezetAb32760 used on Mouse Cortex and Hippocampus in free floating sections using direct fluorescence. The tissues were fixed (animals perfused fixed) with 4% PFA and later postfixed overnight in the same fixative. They were cryoprotected in 30% sucrose and cut using a cryostat. -
Immunoprecipitation - Anti-Myelin oligodendrocyte glycoprotein antibody (ab32760)Myelin oligodendrocyte glycoprotein was immunoprecipitated using 0.5mg Mouse Brain tissue lysate, 5µg of Rabbit polyclonal to Myelin oligodendrocyte glycoprotein and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Mouse Brain tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab32760.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 27kDa: Myelin oligodendrocyte glycoprotein
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (28)
ab32760 は 28 報の論文で使用されています。
- Reimann RR et al. Rapid ex vivo reverse genetics identifies the essential determinants of prion protein toxicity. Brain Pathol 33:e13130 (2023). PubMed: 36329611
- Rowland ME et al. Systemic and intrinsic functions of ATRX in glial cell fate and CNS myelination in male mice. Nat Commun 14:7090 (2023). PubMed: 37925436
- You Y et al. Human neural cell type-specific extracellular vesicle proteome defines disease-related molecules associated with activated astrocytes in Alzheimer's disease brain. J Extracell Vesicles 11:e12183 (2022). PubMed: 35029059
- Sánchez-de la Torre A et al. Cannabinoid CB1 receptor gene inactivation in oligodendrocyte precursors disrupts oligodendrogenesis and myelination in mice. Cell Death Dis 13:585 (2022). PubMed: 35798697
- Khandker L et al. Cholesterol biosynthesis defines oligodendrocyte precursor heterogeneity between brain and spinal cord. Cell Rep 38:110423 (2022). PubMed: 35235799