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Anti-Myelin Basic Protein 抗体 [12] - BSA and Azide free (ab277483)

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Immunocytochemistry/ Immunofluorescence - Anti-Myelin Basic Protein antibody [12] - BSA and Azide free (ab277483)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [12] - BSA and Azide free (ab277483)
  • Western blot - Anti-Myelin Basic Protein antibody [12] - BSA and Azide free (ab277483)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [12] - BSA and Azide free (ab277483)
  • Western blot - Anti-Myelin Basic Protein antibody [12] - BSA and Azide free (ab277483)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rat monoclonal [12] to Myelin Basic Protein - BSA and Azide free
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Unconjugated

製品の概要

  • 製品名

    Anti-Myelin Basic Protein antibody [12] - BSA and Azide free
    Myelin Basic Protein 一次抗体 製品一覧

  • 製品の詳細

    Rat monoclonal [12] to Myelin Basic Protein - BSA and Azide free

  • 由来種

    Rat

  • アプリケーション

    適用あり: WB, IHC-P, ICC/IFmore details

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Full length protein corresponding to Cow Myelin Basic Protein.

  • エピトープ

    Amino acids 82-87 (DENPVV).

  • ポジティブ･コントロール

    • WB: Human cerebellum tissue lysate; Mouse brain tissue lysate; Rat brain tissue lysate. IHC-P: Human cerebellum tissue. ICC/IF: Mouse primary oligodendroglia cells.

  • 特記事項

    ab277483 is the carrier-free version of ab7349.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab277483の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
Use at an assay dependent concentration.
特記事項
WB
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
Use at an assay dependent concentration.

ターゲット情報

  • 機能

    The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation.

  • 組織特異性

    MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system.

  • 関連疾患

    Note=The reduction in the surface charge of citrullinated and/or methylated MBP could result in a weakened attachment to the myelin membrane. This mechanism could be operative in demyelinating diseases such as chronical multiple sclerosis (MS), and fulminating MS (Marburg disease).

  • 配列類似性

    Belongs to the myelin basic protein family.

  • 発生段階

    Expression begins abruptly in 14-16 week old fetuses. Even smaller isoforms seem to be produced during embryogenesis; some of these persisting in the adult. Isoform 4 expression is more evident at 16 weeks and its relative proportion declines thereafter.

  • 翻訳後修飾

    Several charge isomers of MBP; C1 (the most cationic, least modified, and most abundant form), C2, C3, C4, C5, C6, C7, C8-A and C8-B (the least cationic form); are produced as a result of optional PTM, such as phosphorylation, deamidation of glutamine or asparagine, arginine citrullination and methylation. C8-A and C8-B contain each two mass isoforms termed C8-A(H), C8-A(L), C8-B(H) and C8-B(L), (H) standing for higher and (L) for lower molecular weight. C3, C4 and C5 are phosphorylated. The ratio of methylated arginine residues decreases during aging, making the protein more cationic.
    The N-terminal alanine is acetylated (isoform 3, isoform 4, isoform 5 and isoform 6).
    Arg-241 was found to be 6% monomethylated and 60% symmetrically dimethylated.

  • 細胞内局在

    Myelin membrane. Cytoplasmic side of myelin.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • GDB antibody
    • Golli MBP antibody
    • Golli MBP; myelin basic protein antibody
    • Hemopoietic MBP antibody
    • HMBPR antibody
    • HUGO antibody
    • MBP antibody
    • MBP_CAVPO antibody
    • MBP_HUMAN antibody
    • MGC99675 antibody
    • MLD antibody
    • Myelin A1 protein antibody
    • Myelin A1 Protein, basic antibody
    • Myelin basic protein antibody
    • Myelin Deficient antibody
    • Myelin membrane encephalitogenic protein antibody
    • OTTHUMP00000163776 antibody
    • OTTHUMP00000174387 antibody
    • OTTHUMP00000174388 antibody
    • SHI antibody
    • Shiverer antibody
    • SP antibody
    see all

画像

  • Immunocytochemistry/ Immunofluorescence - Anti-Myelin Basic Protein antibody [12] - BSA and Azide free (ab277483)
    Immunocytochemistry/ Immunofluorescence - Anti-Myelin Basic Protein antibody [12] - BSA and Azide free (ab277483)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized mouse primary neural/glia cells labelling Myelin Basic Protein with ab7349 at a 1/200 dilution, followed by Goat anti-Rat secondary (Alexa Fluor® 488) (ab150157) secondary antibody at a 1/1000 dilution. Confocal image showing positive staining in mouse primary oligodendroglia cells (shown in green). Nuclear DNA was labeled with DAPI (shown in blue).

    Counterstained with Anti-MAP2 antibody (ab183830) at a 1/1000 dilution, followed by Goat Anti-Rabbit secondary (Alexa Fluor® 594) (ab150080) at a 1/1000 dilution (shown in magenta).

    Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    The negative controls are as follows:
    -ve control 1: Myelin Basic Protein (ab7349) at a 1/200 dilution, followed by Goat anti-Rabbit secondary (Alexa Fluor® 594) (ab150080) at a 1/1000 dilution. 
    -ve control 2: Anti-MAP2 (ab183830) at a 1/1000 dilution, followed by Goat anti-Rat secondary (Alexa Fluor® 488) (ab150157) at a 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab7349).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [12] - BSA and Azide free (ab277483)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [12] - BSA and Azide free (ab277483)

    This data was developed using the ab7349, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human cerebrum labeling Myelin Basic Protein with ab7349 at 1/4000 dilution (0.259 µg/ml) followed by a ready to use Goat Anti-Rat IgG H&L (HRP polymer; ab214882). The section was incubated with ab7349 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Western blot - Anti-Myelin Basic Protein antibody [12] - BSA and Azide free (ab277483)
    Western blot - Anti-Myelin Basic Protein antibody [12] - BSA and Azide free (ab277483)
    Anti-Myelin Basic Protein antibody [12] (ab7349) at 1/5000 dilution + Human cerebellum tissue lysate at 10 µg

    Secondary
    Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/10000 dilution

    Developed using the ECL technique.

    Observed band size: 18.5 kDa why is the actual band size different from the predicted?


    Exposure time: 1 second


    This data was developed using ab7349, the same antibody clone in a different buffer formulation.

    The molecular weight observed was in consistency with the literature (PMID: 9299539).

    Blocking buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [12] - BSA and Azide free (ab277483)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [12] - BSA and Azide free (ab277483)

    This data was developed using the ab7349, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human cerebrum labeling Myelin Basic Protein with ab7349 at 1/4000 dilution (0.259 µg/ml) followed by a ready to use Goat Anti-Rat IgG H&L (HRP polymer; ab214882). The section was incubated with ab7349 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with DAPI.

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Western blot - Anti-Myelin Basic Protein antibody [12] - BSA and Azide free (ab277483)
    Western blot - Anti-Myelin Basic Protein antibody [12] - BSA and Azide free (ab277483)
    All lanes : Anti-Myelin Basic Protein antibody [12] (ab7349) at 1/5000 dilution

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Rat brain tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/10000 dilution

    Observed band size: 14-21.5 kDa why is the actual band size different from the predicted?



    This data was developed using the ab7349, the same antibody clone in a different buffer formulation.

    The molecular weight observed was in consistency with the literature (PMID: 9299539).

    Blocking buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 3 seconds; Lane 2: 1 second.

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (0)

ab277483 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab277483 は論文での使用が確認できていません。

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