Anti-MyD88 抗体 (ab2064)
Key features and details
- Rabbit polyclonal to MyD88
- Suitable for: IHC-P, WB, ICC/IF
- Knockout validated
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
-
製品名
Anti-MyD88 antibody
MyD88 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to MyD88 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WB, ICC/IFmore details -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic peptide corresponding to Human MyD88 aa 250-350 (C terminal).
-
ポジティブ･コントロール
- WB: HEK293, HT29, K562, HepG2, A549 and Jurkat whole cell lysate (ab7899). ICC/IF: HeLa, Jurkat and K562 cells.
-
特記事項
MyD88 is a general adapter protein for the Toll/IL-1R family of receptors and plays an important role in the inflammatory response induced by cytokines IL-1 and IL-18 and endotoxin. MyD88 gene is expressed in many tissues.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
KO cell lines
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab2064の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | (1) |
Use a concentration of 10 - 20 µg/ml.
|
| WB | (9) |
1/500 - 1/1000. Detects a band of approximately 35 kDa (predicted molecular weight: 33 kDa).
|
| ICC/IF |
Use a concentration of 20 µg/ml.
|
| 特記事項 |
|---|
|
IHC-P
Use a concentration of 10 - 20 µg/ml. |
|
WB
1/500 - 1/1000. Detects a band of approximately 35 kDa (predicted molecular weight: 33 kDa). |
|
ICC/IF
Use a concentration of 20 µg/ml. |
ターゲット情報
-
機能
Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response. Acts via IRAK1, IRAK2, IRF7 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Increases IL-8 transcription. Involved in IL-18-mediated signaling pathway. -
組織特異性
Ubiquitous. -
関連疾患
Defects in MYD88 are the cause of MYD88 deficiency (MYD88D) [MIM:612260]; also known as recurrent pyogenic bacterial infections due to MYD88 deficiency. Patients suffer from autosomal recessive, life-threatening, often recurrent pyogenic bacterial infections, including invasive pneumococcal disease, and die between 1 and 11 months of age. Surviving patients are otherwise healthy, with normal resistance to other microbes, and their clinical status improved with age. -
配列類似性
Contains 1 death domain.
Contains 1 TIR domain. -
ドメイン
The intermediate domain (ID) is required for the phosphorylation and activation of IRAK. -
細胞内局在
Cytoplasm. - Information by UniProt
-
参照データベース
- Entrez Gene: 4615 Human
- Entrez Gene: 17874 Mouse
- Omim: 602170 Human
- SwissProt: Q99836 Human
- SwissProt: P22366 Mouse
- Unigene: 82116 Human
- Unigene: 213003 Mouse
-
別名
- Mutant myeloid differentiation primary response 88 antibody
- MYD 88 antibody
- Myd88 antibody
see all
画像
-
Immunocytochemistry/ Immunofluorescence - Anti-MyD88 antibody (ab2064)
Immunofluorescent analysis of methanol-fixed HeLa cells labeling MyD88 with ab2064 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and Hoechst staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green).
-
Western blot - Anti-MyD88 antibody (ab2064)All lanes : Anti-MyD88 antibody (ab2064) at 2 µg/ml
Lane 1 : HeLa WT cell lysate
Lane 2 : MyD88 KO HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 33 kDa1 h incubation at RT in 5% NFDM/TBST.
beta-actin was used as a loading control at 1 µg/mL.
-
Western blot - Anti-MyD88 antibody (ab2064)All lanes : Anti-MyD88 antibody (ab2064) at 1/1000 dilution
Lane 1 : Wild-type A549 cell lysate
Lane 2 : MYD88 knockout A549 cell lysate
Lane 3 : HEK-293 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 33 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?Western blot: Anti-MYD88 antibody (ab2064) staining at 1/1000 dilution, shown in green; Mouse anti-CANX [CANX/1543] (ab238078) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab2064 was shown to bind specifically to MYD88. A band was observed at 35 kDa in wild-type A549 cell lysates with no signal observed at this size in MYD88 knockout cell line ab286715 (knockout cell lysate ab290793). To generate this image, wild-type and MYD88 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5% milk in TBS-0.1% Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
-
Western blot - Anti-MyD88 antibody (ab2064)All lanes : Anti-MyD88 antibody (ab2064) at 2 µg/ml
Lane 1 : A549 cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : K562 cell lysate
Lane 4 : HT29 cell lysate
Lane 5 : HEK293 cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 33 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-MyD88 antibody (ab2064)Immunocytochemistry/ Immunofluorescence analysis of 4% paraformaldehyde fixed Jurkat cells labeling MyD88 with ab2064 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue). -
Immunocytochemistry/ Immunofluorescence - Anti-MyD88 antibody (ab2064)Immunocytochemistry/ Immunofluorescence analysis of 4% paraformaldehyde fixed K562 cells labeling MyD88 with ab2064 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue). -
Western blot - Anti-MyD88 antibody (ab2064)This image is courtesy of an anonymous AbreviewAll lanes : Anti-MyD88 antibody (ab2064) at 1 µg/ml
Lanes 1-2 : Wild type MEFs whole cell lysate
Lanes 3-4 : MyD88 knockout MEFs whole cell lysate
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG polyclonal at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 33 kDa
Observed band size: 33 kDa
Exposure time: 15 seconds
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (147)
ab2064 は 147 報の論文で使用されています。
- Jiang H et al. Acupuncture Ameliorates Depression-Like Behaviors Through Modulating the Neuroinflammation Mediated by TLR4 Signaling Pathway in Rats Exposed to Chronic Restraint Stress. Mol Neurobiol 61:2606-2619 (2024). PubMed: 37917302
- Huang J et al. Effect of curcumin on regulatory B cells in chronic colitis mice involving TLR/MyD88 signaling pathway. Phytother Res 37:731-742 (2023). PubMed: 36196887
- Yao XP et al. Adaptor protein MyD88 confers the susceptibility to stress via amplifying immune danger signals. Brain Behav Immun 108:204-220 (2023). PubMed: 36496170
- Gong R et al. Electroacupuncture-induced activation of GABAergic system alleviates airway inflammation in asthma model by suppressing TLR4/MyD88/NF-κB signaling pathway. Chin Med J (Engl) 136:451-460 (2023). PubMed: 36867547
- González-Carnicero Z et al. Regulation by Nrf2 of IL-1β-induced inflammatory and oxidative response in VSMC and its relationship with TLR4. Front Pharmacol 14:1058488 (2023). PubMed: 36937865