Anti-Myc tag 抗体 [Hyper-myc] - Mouse IgG2a (Chimeric) - BSA and Azide free (ab290005)

This data was developed using ab289980, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST

ab289980 detects a ranged of myc tagged proteins in WB.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T cells transfected with CRISPR-Cas9 (S. Aureas) expression vector containing a myc tag, labeling myc-tag with ab289980 at 1/500 (2.202 µg/ml) dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor^® 488) (ab150117) preadsorbed secondary antibody at 1/1000 dilution (Green). Confocal image showing positive staining in HEK-293T cells transfected with CRISPR-Cas9 (S. Aureas) expression vector containing a myc tag.

Nulcear counterstain is DAPI. Cells were also counterstained with ab203933 Anti-CRISPR-Cas9 rabbit monoclonal antibody at 1/500 (2 µg/ml) dilution, followed by secondary antibody ab203933 Anti-CRISPR-Cas9 rabbit monoclonal antibody at 1/200 (2.5 µg/ml) dilution. 

-ve control 1: ab289980 at 1/500 (2.202 µg/ml) dilution, followed by ab15088 1/200 (2.5 µg/ml)

-ve control 2: ab15088 1/200 (2 µg/ml), followed by ab150117 1/1000 (2 µg/ml)

This data was developed using ab289980, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of 293T (human embryonic kidney) transfected with TMEM-119 expression vector containing a myc-His-tag®, fixed with 2% paraformaldehyde and permeabelized 0.1% Tween-20. Cells were surface stained with TMEM-119 conjugated with Alexa Fluor® 647 (ab225494). Then fixed with 2% PFA for 10min followed by intracellularly stained with mouse IgG or ab289980. ab289980 was used at 1:1000 dilution (0.1μg)(Right) compared with Mouse monoclonal IgG (Left). Secondary antibody used was Goat anti-Mouse IgG (Alexa Fluor® 488, ab150113) at 1/2000 dilution. 

This data was developed using ab289980, the same antibody clone in a different buffer formulation.

Myc-tag was immunoprecipitated from HEK-293T transfected with human NF2 S518A expression vector containing a myc-His-tag® whole cell lysate (2µg). Western blot was performed using immunopreciptate, ab289980 was used at 1/30 dilution (2µg in 0.35mg lysates). Secondary antibody used was Veriblot for IP secondary antibody (HRP) (ab131366) at 1/5000 dilution. 

Lane 1 (Input): HEK-293T transfected with human NF2 S518A expression vector containing a myc-His-tag® whole cell lysate, 2µg.

Lane 2 (+): HEK-293T transfected with human NF2 S518A expression vector containing a myc-His-tag® whole cell lysate

Lane 3 (-):  Human IgG instead of ab289980 in HEK-293T transfected with human NF2 S518A expression vector containing a myc-His-tag® whole cell lysate

Blocking and dilution buffer: 5% NFDM/TBST. 

ab289980 detects a ranged of myc tagged proteins in WB.

This data was developed using ab289980, the same antibody clone in a different buffer formulation.

Indirect ELISA antibody dose-response curve of ab289980 for Human myc-tag antigen, at a concentration of 1000ng/ml. Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) at 1/1000 dilution.