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リコンビナント

Anti-Myc tag 抗体 [Hyper-myc] (ab289981)

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Western blot - Anti-Human monoclonal [Hyper-myc] to Myc tag antibody [Hyper-myc] (ab289981)
  • Immunocytochemistry/ Immunofluorescence - Anti-Myc tag antibody [Hyper-myc] (ab289981)
  • Immunoprecipitation - Anti-Human monoclonal [Hyper-myc] to Myc tag antibody [Hyper-myc] (ab289981)
  • Indirect ELISA - Anti-Myc tag antibody [Hyper-myc] (ab289981)
  • Anti-Myc tag antibody [Hyper-myc] (ab289981)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Human monoclonal [Hyper-myc] to Myc tag
  • Suitable for: IP, WB, ICC/IF, Indirect ELISA
  • Reacts with: Species independent

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-Myc tag antibody [Hyper-myc]
    Myc tag 一次抗体 製品一覧

  • 製品の詳細

    Human monoclonal [Hyper-myc] to Myc tag

  • 由来種

    Human

  • アプリケーション

    適用あり: IP, WB, ICC/IF, Indirect ELISAmore details
    適用なし: Flow Cyt (Intra)

  • 種交差性

    交差種: Species independent

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: HEK-293T whole cell lysates transfected with: cas9 (S. Aureas) expression vector with a myc-His-tag®, human SNCA140 expression vector containing a myc-His-tag®, human IDS expression vector containing a myc-His-tag®, human NF2 S518A expression vector containing a myc-His-tag®, human Alpha-synuclein expression vector containing a myc-His-tag®. HEK-293T. IP: HEK-293T transfected with human NF2 S518A expression vector containing a myc-His-tag® whole cell lysate. Indirect ELISA: Human myc-tag

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    Hyper-myc

  • アイソタイプ

    IgG1

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab289981の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IP
1/30.
WB
1/5000. Predicted molecular weight: 49 kDa.
ICC/IF
1/8000.
Indirect ELISA
Use at an assay dependent concentration.
特記事項
IP
1/30.
WB
1/5000. Predicted molecular weight: 49 kDa.
ICC/IF
1/8000.
Indirect ELISA
Use at an assay dependent concentration.
追加情報
Is unsuitable for Flow Cyt (Intra).

ターゲット情報

  • 関連性

    Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.

  • 細胞内局在

    Nuclear

  • 別名

    • c-myc tag antibody
    • Myc Epitope Tag antibody

画像

  • Western blot - Anti-Human monoclonal [Hyper-myc] to Myc tag antibody [Hyper-myc] (ab289981)
    Western blot - Anti-Human monoclonal [Hyper-myc] to Myc tag antibody [Hyper-myc] (ab289981)
    All lanes : Anti-Myc tag antibody [Hyper-myc] (ab289981) at 1/5000 dilution

    Lane 1 : HEK-293T (human embryonic kidney) transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate
    Lane 2 : HEK-293T transfected with cas9 ( S. Aureas) expression vector containing a myc-His-tag®, whole cell lysate
    Lane 3 : HEK-293T transfected with human SNCA140 expression vector containing a myc-His-tag®, whole cell lysate
    Lane 4 : HEK-293T transfected with human IDS expression vector containing a myc-His-tag®, whole cell lysate
    Lane 5 : HEK-293T transfected with human NF2 S518A expression vector containing a myc-His-tag®, whole cell lysate
    Lane 6 : HEK-293T transfected with human Alpha-synuclein expression vector containing a myc-His-tag®, whole cell lysate

    Lysates/proteins at 1 µg per lane.

    Secondary
    All lanes : Goat Anti-Human IgG Fc (HRP) preadsorbed (ab98624) at 1/10000 dilution

    Predicted band size: 49 kDa


    Exposure time: 1 second


    Blocking and diluting buffer and concnetration are 5% NFDM/TBST. 

    ab289981 detects a ranged of myc tagged proteins in WB. 

  • Immunocytochemistry/ Immunofluorescence - Anti-Myc tag antibody [Hyper-myc] (ab289981)
    Immunocytochemistry/ Immunofluorescence - Anti-Myc tag antibody [Hyper-myc] (ab289981)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (human embryonic kidney) whole cell lysate labelingMyc tag with ab289981 at 1/8000 dilution, followed by Goat anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody (Alexa Fluor® 488) secondary antibody at 1/500 dilution (green). Confocal image showing positive staining in HEK-293T cells transfected with CRISPR-Cas9 (S. Aureas) expression vector containing a myc tag. The nuclear counter stain is DAPI (blue).

    Anti-CRISPR-Cas9 rabbit monoclonal antibody (ab203933) at 1/500 dilution was used as counterstain followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (ab150088) (red). 


    The negative controls are as follows:
    -ve control 1: ab289981 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (ab150088) secondary antibody at 1/200 dilution.
    -ve control 2: ab203933 at 1/500 dilution, followed by Goat anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody (Alexa Fluor® 488) at 1/500 dilution.

  • Immunoprecipitation - Anti-Human monoclonal [Hyper-myc] to Myc tag antibody [Hyper-myc] (ab289981)
    Immunoprecipitation - Anti-Human monoclonal [Hyper-myc] to Myc tag antibody [Hyper-myc] (ab289981)

    Myc tag was immunoprecipitated from HEK-293T transfected with human NF2 S518A expression vector containing a myc-His-tag® whole cell lysate with ab289981 at 1/30 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. 

    Lane 1 (Input): HEK-293T transfected with human NF2 S518A expression vector containing a myc-His-tag® whole cell lysate 2 μg

    Lane 2 (+): HEK-293T transfected with human NF2 S518A expression vector containing a myc-His-tag® whole cell lysate

    Lane 3 (-): Human IgG instead of ab289981 in HEK-293T transfected with human NF2 S518A expression vector containing a myc-His-tag® whole cell lysate. 

  • Indirect ELISA - Anti-Myc tag antibody [Hyper-myc] (ab289981)
    Indirect ELISA - Anti-Myc tag antibody [Hyper-myc] (ab289981)

    Indirect ELISA antibody dose-response curve using ab289981 with antigen at 1000 ng/ml. 

  • Anti-Myc tag antibody [Hyper-myc] (ab289981)
    Anti-Myc tag antibody [Hyper-myc] (ab289981)

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (0)

ab289981 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab289981 は論文での使用が確認できていません。

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