Anti-Myc tag 抗体 - ChIP Grade (ab9132)
Key features and details
- Goat polyclonal to Myc tag
- Suitable for: IP, ChIP, WB, ICC, ELISA
- Isotype: IgG
製品の概要
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製品名
Anti-Myc tag antibody - ChIP Grade
Myc tag 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to Myc tag -
由来種
Goat -
特異性
Antibody was verified by ELISA against peptide conjugated to BSA (EQKLISEEDL /BSA). -
アプリケーション
適用あり: IP, ChIP, WB, ICC, ELISAmore details -
免疫原
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 6.80
Preservative: 0.1% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Antibodies were affinity purified using the peptide immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at280 nm of 1.4 equals 1.0 mg of IgG. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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ChIP Related Products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab9132の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IP |
Use at 1-4 µg/mg of lysate.
Also PubMed: 20224777
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| ChIP | (2) |
Use at an assay dependent concentration. ChIP was performed with 25 ug chromatin, 5ug of antibody and 20 ul of Protein A/G beads.
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| WB | (3) |
1/1000 - 1/30000.
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| ICC |
1/200 - 1/2000.
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| ELISA |
1/100 - 1/500.
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| 特記事項 |
|---|
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IP
Use at 1-4 µg/mg of lysate. Also PubMed: 20224777
|
|
ChIP
Use at an assay dependent concentration. ChIP was performed with 25 ug chromatin, 5ug of antibody and 20 ul of Protein A/G beads. |
|
WB
1/1000 - 1/30000. |
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ICC
1/200 - 1/2000. |
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ELISA
1/100 - 1/500. |
ターゲット情報
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関連性
Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells. -
細胞内局在
Nuclear -
別名
- c-myc tag antibody
- Myc Epitope Tag antibody
画像
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Western blot - Anti-Myc tag antibody - ChIP Grade (ab9132)All lanes : Anti-Myc tag antibody - ChIP Grade (ab9132) at 0.04 µg/ml
Lane 1 : E. coli whole cell lysate at 0.2 µg
Lane 2 : E. coli whole cell lysate at 0.1 µg
Lane 3 : E. coli whole cell lysate at 0.05 µg -
ChIP - Anti-Myc tag antibody - ChIP Grade (ab9132)A stably transfected 293T human cell line harbouring the GAL4 upstream activation sequence was transiently transfected with a Myc or His - tagged GAL4 DNA Binding Domain construct. 48 hours post transfection Chromatin was prepared according to the Abcam X-ChIP protocol. The ChIP was performed with 25 ug chromatin, 5ug of antibody and 20 ul of Protein A/G beads. A non-specific antibody was used as the negative control. The immunoprecipitated DNA was quantified by real time PCR (SYBR Green approach).
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (151)
ab9132 は 151 報の論文で使用されています。
- Higerd-Rusli GP et al. The fates of internalized NaV1.7 channels in sensory neurons: Retrograde cotransport with other ion channels, axon-specific recycling, and degradation. J Biol Chem 299:102816 (2023). PubMed: 36539035
- Ballmer D et al. HP1 proteins regulate nucleolar structure and function by secluding pericentromeric constitutive heterochromatin. Nucleic Acids Res 51:117-143 (2023). PubMed: 36533441
- Palicharla VR et al. Interactions between TULP3 tubby domain and ARL13B amphipathic helix promote lipidated protein transport to cilia. Mol Biol Cell 34:ar18 (2023). PubMed: 36652335
- Dou C et al. Sialylation of TLR2 initiates osteoclast fusion. Bone Res 10:24 (2022). PubMed: 35232979
- Ermakova M et al. Enhanced abundance and activity of the chloroplast ATP synthase in rice through the overexpression of the AtpD subunit. J Exp Bot 73:6891-6901 (2022). PubMed: 35904136