Key features and details
- Chicken polyclonal to Myc tag
- Suitable for: WB, IP, ICC
- Isotype: IgY
- 倫理基準に準拠 - アニマル・フリーの生産
製品名Anti-Myc tag antibody
Myc tag 一次抗体 製品一覧
製品の詳細Chicken polyclonal to Myc tag
アプリケーション適用あり: WB, IP, ICCmore details
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium azide
Concentration information loading...
特記事項（精製）Antibodies were purified from the yolks using a proprietary method that yields a purity of >90%. Antibodies were affinity-purified over a SulfoLink® (Pierce) column conjugated with the peptide used for immunizations, and then dialyzed against PBS. Filter-sterilized (0.2 µm).
関連性Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
- c-myc tag antibody
- Myc Epitope Tag antibody
All lanes : Anti-Myc tag antibody (ab172)
Lane 1 : Cultured normal mouse embryo fibroblasts were transfected with a plasmid containing the SAP1a cDNA fused with a c-myc epitope tag at its C-terminus. at 30 µg
Lane 2 : Cultured normal mouse embryo fibroblasts were transfected with a plasmid containing the SAP1a cDNA fused with a c-myc epitope tag at its C-terminus. at 20 µg
Lane 3 : Cultured normal mouse embryo fibroblasts were transfected with a plasmid containing the SAP1a cDNA fused with a c-myc epitope tag at its C-terminus. at 10 µg
Lane 4 : Cultured normal mouse embryo fibroblasts were transfected with a plasmid containing the SAP1a cDNA fused with a c-myc epitope tag at its C-terminus. at 5 µg
ab172 は 7 報の論文で使用されています。
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- Bréchet A et al. Protein kinase CK2 contributes to the organization of sodium channels in axonal membranes by regulating their interactions with ankyrin G. J Cell Biol 183:1101-14 (2008). ICC/IF . PubMed: 19064667