Anti-Myc tag 抗体 (ab172)
Key features and details
- Chicken polyclonal to Myc tag
- Suitable for: WB, IP, ICC
- Isotype: IgY
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Myc tag antibody
Myc tag 一次抗体 製品一覧 -
製品の詳細
Chicken polyclonal to Myc tag -
由来種
Chicken -
アプリケーション
適用あり: WB, IP, ICCmore details -
免疫原
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7
Preservative: 0.02% Sodium azide -
Concentration information loading...
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精製度
IgY fraction -
特記事項(精製)
Antibodies were purified from the yolks using a proprietary method that yields a purity of >90%. Antibodies were affinity-purified over a SulfoLink® (Pierce) column conjugated with the peptide used for immunizations, and then dialyzed against PBS. Filter-sterilized (0.2 µm). -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgY -
研究分野
関連製品
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab172の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB | (1) |
1/500.
|
IP |
1/250.
|
|
ICC |
1/250.
|
特記事項 |
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WB
1/500. |
IP
1/250. |
ICC
1/250. |
ターゲット情報
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関連性
Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells. -
細胞内局在
Nuclear -
別名
- c-myc tag antibody
- Myc Epitope Tag antibody
画像
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All lanes : Anti-Myc tag antibody (ab172)
Lane 1 : Cultured normal mouse embryo fibroblasts were transfected with a plasmid containing the SAP1a cDNA fused with a c-myc epitope tag at its C-terminus. at 30 µg
Lane 2 : Cultured normal mouse embryo fibroblasts were transfected with a plasmid containing the SAP1a cDNA fused with a c-myc epitope tag at its C-terminus. at 20 µg
Lane 3 : Cultured normal mouse embryo fibroblasts were transfected with a plasmid containing the SAP1a cDNA fused with a c-myc epitope tag at its C-terminus. at 10 µg
Lane 4 : Cultured normal mouse embryo fibroblasts were transfected with a plasmid containing the SAP1a cDNA fused with a c-myc epitope tag at its C-terminus. at 5 µg
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (8)
ab172 は 8 報の論文で使用されています。
- Zou QT et al. miR-138-5p inhibits the progression of colorectal cancer via regulating SP1/LGR5 axis. Cell Biol Int 47:273-282 (2023). PubMed: 36317454
- Ma T et al. HOXA10 promotion of HDAC1 underpins the development of lung adenocarcinoma through the DNMT1-KLF4 axis. J Exp Clin Cancer Res 40:71 (2021). PubMed: 33596966
- Wan J et al. MYB70 modulates seed germination and root system development in Arabidopsis. iScience 24:103228 (2021). PubMed: 34746697
- Puri R et al. Mul1 restrains Parkin-mediated mitophagy in mature neurons by maintaining ER-mitochondrial contacts. Nat Commun 10:3645 (2019). PubMed: 31409786
- Czarna A et al. Single-cell analysis of the fate of c-kit-positive bone marrow cells. NPJ Regen Med 2:27 (2017). PubMed: 29302361
- Rutkowska-Wlodarczyk I et al. A proteomic analysis reveals the interaction of GluK1 ionotropic kainate receptor subunits with Go proteins. J Neurosci 35:5171-9 (2015). PubMed: 25834043
- Selak S et al. A role for SNAP25 in internalization of kainate receptors and synaptic plasticity. Neuron 63:357-71 (2009). ICC/IF . PubMed: 19679075
- Bréchet A et al. Protein kinase CK2 contributes to the organization of sodium channels in axonal membranes by regulating their interactions with ankyrin G. J Cell Biol 183:1101-14 (2008). ICC/IF . PubMed: 19064667