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リコンビナントRabMAb

Anti-MXD3/MAD3 抗体 [EPR3882] (ab108525)

Submit a review Submit a question References (2)
Western blot - Anti-MXD3/MAD3 antibody [EPR3882] (ab108525)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MXD3/MAD3 antibody [EPR3882] (ab108525)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MXD3/MAD3 antibody [EPR3882] (ab108525)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MXD3/MAD3 antibody [EPR3882] (ab108525)
  • Anti-MXD3/MAD3 antibody [EPR3882] (ab108525)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3882] to MXD3/MAD3
  • Suitable for: WB, IHC-P
  • Reacts with: Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-MXD3/MAD3 antibody [EPR3882]
    MXD3/MAD3 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR3882] to MXD3/MAD3

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-Pmore details
    適用なし: ICC/IF or IP

  • 種交差性

    交差種: Rat, Human
    交差が予測される動物種: Mouse

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • HeLa and C6 cell lysates. Human brain, breast and testis tissue.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab108525の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 23 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

For antigen retrieval, heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.
特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 23 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

For antigen retrieval, heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.
追加情報
Is unsuitable for ICC/IF or IP.

ターゲット情報

  • 機能

    Transcriptional repressor. Binds with MAX to form a sequence-specific DNA-binding protein complex which recognizes the core sequence 5'-CAC[GA]TG-3'. Antagonizes MYC transcriptional activity by competing for MAX and suppresses MYC dependent cell transformation.

  • 配列類似性

    Contains 1 basic helix-loop-helix (bHLH) domain.

  • 細胞内局在

    Nucleus.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • bHLHc13 antibody
    • Class C basic helix-loop-helix protein 13 antibody
    • FLJ35523 antibody
    • Likely ortholog of mouse Max dimerization protein 3 antibody
    • MAD3_HUMAN antibody
    • MAX dimerization protein 3 antibody
    • Max dimerizer 3 antibody
    • Max-associated protein 3 antibody
    • Max-interacting transcriptional repressor MAD3 antibody
    • MGC2383 antibody
    • mxd3 antibody
    • Myx antibody
    • OTTHUMP00000223596 antibody
    • OTTHUMP00000223598 antibody
    see all

画像

  • Western blot - Anti-MXD3/MAD3 antibody [EPR3882] (ab108525)
    Western blot - Anti-MXD3/MAD3 antibody [EPR3882] (ab108525)
    All lanes : Anti-MXD3/MAD3 antibody [EPR3882] (ab108525) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : C6 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP-labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 23 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MXD3/MAD3 antibody [EPR3882] (ab108525)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MXD3/MAD3 antibody [EPR3882] (ab108525)

    ab108525 at 1/100 dilution staining MAD3 in paraffin-embedded Human brain tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MXD3/MAD3 antibody [EPR3882] (ab108525)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MXD3/MAD3 antibody [EPR3882] (ab108525)

    ab108525 at 1/100 dilution staining MAD3 in paraffin-embedded Human breast tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MXD3/MAD3 antibody [EPR3882] (ab108525)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MXD3/MAD3 antibody [EPR3882] (ab108525)

    ab108525 at 1/100 dilution staining MAD3 in paraffin-embedded Human testis tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-MXD3/MAD3 antibody [EPR3882] (ab108525)
    Anti-MXD3/MAD3 antibody [EPR3882] (ab108525)

参考文献 (2)

ab108525 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab108525 は 2 報の論文で使用されています。

  • Zhang X  et al. MXD3 as an Immunological and Prognostic Factor From Pancancer Analysis. Front Mol Biosci 8:702206 (2021). PubMed: 34859046
  • Tsai YW  et al. MXD3 Promotes Obesity and the Androgen Receptor Signaling Pathway in Gender-Disparity Hepatocarcinogenesis. Cells 10:N/A (2021). PubMed: 34943942

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