AB247683

Anti-MXD3/MAD3 抗体 [EPR3882] - BSA and Azide free

Anti-MXD3/MAD3 antibody [EPR3882] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal MXD3/MAD3 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Rat samples. Cited in 1 publication.

別名を表示する

BHLHC13, MAD3, MXD3, Max dimerization protein 3, Max dimerizer 3, Class C basic helix-loop-helix protein 13, Max-associated protein 3, Max-interacting transcriptional repressor MAD3, Myx, bHLHc13

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MXD3/MAD3 antibody [EPR3882] - BSA and Azide free (AB247683)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MXD3/MAD3 antibody [EPR3882] - BSA and Azide free (AB247683)

This data was developed using ab108525, the same antibody clone in a different buffer formulation.

ab108525 at 1/100 dilution staining MAD3 in paraffin-embedded Human testis tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MXD3/MAD3 antibody [EPR3882] - BSA and Azide free (AB247683)

This data was developed using ab108525, the same antibody clone in a different buffer formulation.

ab108525 at 1/100 dilution staining MAD3 in paraffin-embedded Human breast tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MXD3/MAD3 antibody [EPR3882] - BSA and Azide free (AB247683)

This data was developed using ab108525, the same antibody clone in a different buffer formulation.

ab108525 at 1/100 dilution staining MAD3 in paraffin-embedded Human brain tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-MXD3/MAD3 antibody [EPR3882] - BSA and Azide free (AB247683)

This data was developed using ab108525, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-MXD3/MAD3 antibody [EPR3882] (<a href='/products/primary-antibodies/mxd3-mad3-antibody-epr3882-ab108525'>ab108525</a>) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

C6 cell lysate at 10 µg

Secondary

All lanes:

HRP-labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 23 kDa

false

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR3882

アイソタイプ

IgG

キャリアフリー

Yes

交差種

Rat, Human

アプリケーション

WB, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "Heat up to 98 degree C, below boiling, and then let cool for 10-20 minutes.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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製品の詳細

ab247683 is the carrier-free version of ab108525.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification

バッファー組成

pH: 7.2 - 7.4 Constituents: PBS

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

Do Not Freeze

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Transcriptional repressor. Binds with MAX to form a sequence-specific DNA-binding protein complex which recognizes the core sequence 5'-CAC[GA]TG-3'. Antagonizes MYC transcriptional activity by competing for MAX and suppresses MYC dependent cell transformation (By similarity).

See full target information MXD3

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Advanced materials (Deerfield Beach, Fla.) 35:e2302801 PubMed37589156

2023

Cellular Membrane-Engineered Nanovesicles as a Three-Stage Booster to Target the Lesion Core.

Applications

Unspecified application

Species

Unspecified reactive species

Fanyi Huang,Yawei Du,Yanjia Chen,Zeping Qiu,Zhiyan Wang,Yingze Fan,Yunjing Shi,Zhuojin Li,Ke Yang,Wenguo Cui,Wei Jin

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MXD3/MAD3 抗体 [EPR3882] - BSA and Azide free

Anti-MXD3/MAD3 antibody [EPR3882] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MXD3/MAD3 antibody [EPR3882] - BSA and Azide free (AB247683)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MXD3/MAD3 antibody [EPR3882] - BSA and Azide free (AB247683)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MXD3/MAD3 antibody [EPR3882] - BSA and Azide free (AB247683)

Western blot - Anti-MXD3/MAD3 antibody [EPR3882] - BSA and Azide free (AB247683)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

保管に関する情報

製品プロトコール

ターゲットの情報

文献 (1)

Abcam product promise