Anti-MVP 抗体 [EPR13226(B)] (ab177145)
![Western blot - Anti-MVP antibody [EPR13226(B)] (ab177145)](https://www.abcam.co.jp/ps/products/177/ab177145/Images/ab177145-387273-anti-mvp-antibody-epr13226b-western-blot-hela-a549-molt4-lysates.jpg "Western blot - Anti-MVP antibody [EPR13226(B)] (ab177145)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13226(B)] to MVP
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-MVP antibody [EPR13226(B)]
MVP 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR13226(B)] to MVP -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: Flow Cyt,ICC/IF or IP -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Synthetic peptide within Human MVP aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: Q14764 -
ポジティブ･コントロール
- Human spleen, Calu3 and A549 lysates.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR13226(B) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab177145の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/5000. Detects a band of approximately 110 kDa (predicted molecular weight: 99 kDa).
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| 特記事項 |
|---|
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WB
1/1000 - 1/5000. Detects a band of approximately 110 kDa (predicted molecular weight: 99 kDa). |
ターゲット情報
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機能
Required for normal vault structure. Vaults are multi-subunit structures that may act as scaffolds for proteins involved in signal transduction. Vaults may also play a role in nucleo-cytoplasmic transport. Down-regulates INFG-mediated STAT1 signaling and subsequent activation of JAK. Down-regulates SRC activity and signaling through MAP kinases. -
組織特異性
Present in most normal tissues. Higher expression observed in epithelial cells with secretory and excretory functions, as well as in cells chronically exposed to xenobiotics, such as bronchial cells and cells lining the intestine. Overexpressed in many multidrug-resistant cancer cells. -
配列類似性
Contains 9 MVP (vault) repeats. -
ドメイン
MVP 3 mediates interaction with PTEN.
MVP 4 mediates interaction with PARP4. -
翻訳後修飾
Phosphorylated on Tyr residues after EGF stimulation.
Dephosphorylated by PTPN11. -
細胞内局在
Cytoplasm. Nucleus > nuclear pore complex. 5% found in the nuclear pore complex. Translocates from the nucleus to the cytoplasm upon EGF treatment. - Information by UniProt
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参照データベース
- Entrez Gene: 9961 Human
- Entrez Gene: 78388 Mouse
- Entrez Gene: 64681 Rat
- Omim: 605088 Human
- SwissProt: Q14764 Human
- SwissProt: Q9EQK5 Mouse
- SwissProt: Q62667 Rat
- Unigene: 632177 Human
see all -
別名
- LRP antibody
- Lung resistance related protein antibody
- Lung resistance-related protein antibody
see all
画像
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Western blot - Anti-MVP antibody [EPR13226(B)] (ab177145)All lanes : Anti-MVP antibody [EPR13226(B)] (ab177145) at 1/1000 dilution
Lane 1 : Wild-type HeLa lysate
Lane 2 : MVP knockout HeLa lysate
Lane 3 : A549 lysate
Lane 4 : MOLT-4 lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 99 kDaLanes 1-4: Merged signal (red and green). Green - ab177145 observed at 99 kDa. Red - loading control ab8245 observed at 37 kDa.
ab177145 Anti-MVP antibody [EPR13226(B)] was shown to specifically react with MVP in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264817 (knockout cell lysate ab257544) was used. Wild-type and MVP knockout samples were subjected to SDS-PAGE. ab177145 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-MVP antibody [EPR13226(B)] (ab177145)](https://www.abcam.co.jp/ps/products/177/ab177145/Images/ab177145-205112-anti-mvp-antibody-epr13226b-western-blot.jpg)
Western blot - Anti-MVP antibody [EPR13226(B)] (ab177145)All lanes : Anti-MVP antibody [EPR13226(B)] (ab177145) at 1/1000 dilution
Lane 1 : Human spleen lysate
Lane 2 : Calu3 lysate
Lane 3 : A549 lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 99 kDa -
![Anti-MVP antibody [EPR13226(B)] (ab177145)](https://www.abcam.co.jp/ps/products/177/ab177145/Images/ab177145-3-benefits-of-recombinant-antibodies.png)
Anti-MVP antibody [EPR13226(B)] (ab177145)
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab177145 は論文での使用が確認できていません。