Anti-MVK 抗体 (ab154515)
Key features and details
- Rabbit polyclonal to MVK
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-MVK antibody
MVK 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to MVK -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Recombinant fragment corresponding to a region within amino acids 163-396 of Human MVK (UniProt ID: Q03426).
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ポジティブ･コントロール
- 293T, A431, HepG2, Raji whole cell lysate and A549 xenograft.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab154515の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 42 kDa.
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| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively Tris-EDTA buffer (pH8.0) may be used.
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| 特記事項 |
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WB
1/500 - 1/3000. Predicted molecular weight: 42 kDa. |
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IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively Tris-EDTA buffer (pH8.0) may be used. |
ターゲット情報
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機能
May be a regulatory site in cholesterol biosynthetic pathway. -
パスウェイ
Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via mevalonate pathway; isopentenyl diphosphate from (R)-mevalonate: step 1/3. -
関連疾患
Defects in MVK are the cause of mevalonic aciduria (MEVA) [MIM:610377]. It is an accumulation of mevalonic acid which causes a variety of symptoms such as psychomotor retardation, dysmorphic features, cataracts, hepatosplenomegaly, lymphadenopathy, anemia, hypotonia, myopathy, and ataxia.
Defects in MVK are the cause of hyperimmunoglobulinemia D and periodic fever syndrome (HIDS) [MIM:260920]. HIDS is an autosomal recessive disease characterized by recurrent episodes of unexplained high fever associated with skin rash, diarrhea, adenopathy (swollen, tender lymph nodes), athralgias and/or arthritis. Concentration of IgD, and often IgA, are above normal. -
配列類似性
Belongs to the GHMP kinase family. Mevalonate kinase subfamily. -
細胞内局在
Cytoplasm. Peroxisome. - Information by UniProt
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参照データベース
- Entrez Gene: 4598 Human
- Entrez Gene: 17855 Mouse
- Entrez Gene: 81727 Rat
- Omim: 251170 Human
- SwissProt: Q03426 Human
- SwissProt: Q9R008 Mouse
- SwissProt: P17256 Rat
- Unigene: 130607 Human
see all -
別名
- FLJ96772 antibody
- KIME_HUMAN antibody
- LH receptor mRNA binding protein antibody
see all
画像
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Western blot - Anti-MVK antibody (ab154515)Anti-MVK antibody (ab154515) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size: 42 kDa
10% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MVK antibody (ab154515)Immunohistochemical analysis of formalin-fixed, paraffin-embedded A549 xenograft labeling MVK with ab154515 at 1/500.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab154515 は 1 報の論文で使用されています。
- Vergani E et al. Targeting of the Lipid Metabolism Impairs Resistance to BRAF Kinase Inhibitor in Melanoma. Front Cell Dev Biol 10:927118 (2022). PubMed: 35912092