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Anti-Muscarinic Acetylcholine Receptor M5/CHRM5 抗体 (ab41171)

Submit a review Q&A (3)References (6)

Key features and details

  • Rabbit polyclonal to Muscarinic Acetylcholine Receptor M5/CHRM5
  • Suitable for: ELISA, IHC-FoFr, IHC (PFA fixed), WB
  • Reacts with: Rat
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-Muscarinic Acetylcholine Receptor M5/CHRM5 antibody
    Muscarinic Acetylcholine Receptor M5/CHRM5 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to Muscarinic Acetylcholine Receptor M5/CHRM5

  • 由来種

    Rabbit

  • 特異性

    Cross reactivity: M5 Muscarinic Receptor (519-531): 100% M5 Muscarinic Receptor: 75% No cross reactivity was observed in: M1 Muscarinic Receptor(451-460) M1 Muscarinic Receptor M2 Muscarinic Receptor(457-466) M2 Muscarinic Receptor M3 Muscarinic Receptor(580-589) M3 Muscarinic Receptor M4 Muscarinic Receptor(469-478) M4 Muscarinic Receptor

  • アプリケーション

    適用あり: ELISA, IHC-FoFr, IHC (PFA fixed), WBmore details

  • 種交差性

    交差種: Rat

  • 免疫原

    Synthetic peptide corresponding to Rat Muscarinic Acetylcholine Receptor M5/CHRM5 (C terminal).
    Database link: P08911

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab41171の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ELISA
Use at an assay dependent concentration.
IHC-FoFr
Use at an assay dependent concentration. PubMed: 23773170
IHC (PFA fixed)
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 60 kDa.

Blocking with 5% goat or donkey serum significantly reduces background when compared with BSA or milk blocking.
特記事項
ELISA
Use at an assay dependent concentration.
IHC-FoFr
Use at an assay dependent concentration. PubMed: 23773170
IHC (PFA fixed)
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 60 kDa.

Blocking with 5% goat or donkey serum significantly reduces background when compared with BSA or milk blocking.

ターゲット情報

  • 機能

    The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.

  • 配列類似性

    Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM5 sub-subfamily.

  • 細胞内局在

    Cell membrane. Cell junction, synapse, postsynaptic cell membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Acetylcholine receptor muscarinic 5 antibody
      • AChR antibody
      • AChR M5 antibody
      • ACM5_HUMAN antibody
      • CHKM5MR antibody
      • Cholinergic receptor muscarinic 5 antibody
      • Chrm 5 antibody
      • Chrm5 antibody
      • HM 5 antibody
      • HM5 antibody
      • m5 antibody
      • M5R antibody
      • MGC41838 antibody
      • Muscarinic acetylcholine receptor M5 antibody
      see all

    参考文献 (6)

    ab41171 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab41171 は 6 報の論文で使用されています。

    • Underhill SM & Amara SG Acetylcholine Receptor Stimulation Activates Protein Kinase C Mediated Internalization of the Dopamine Transporter. Front Cell Neurosci 15:662216 (2021). PubMed: 33897375
    • Zhu H  et al. Impairments of spatial memory in an Alzheimer's disease model via degeneration of hippocampal cholinergic synapses. Nat Commun 8:1676 (2017). PubMed: 29162816
    • Muise ED  et al. Localization of muscarinic acetylcholine receptor 2 to the intestinal crypt stem cell compartment. Data Brief 10:482-486 (2017). PubMed: 28054012
    • Muschter D  et al. Reactivity of rat bone marrow-derived macrophages to neurotransmitter stimulation in the context of collagen II-induced arthritis. Arthritis Res Ther 17:169 (2015). IF . PubMed: 26104678
    • Muschter D  et al. Sympathetic Neurotransmitters Modulate Osteoclastogenesis and Osteoclast Activity in the Context of Collagen-Induced Arthritis. PLoS One 10:e0139726 (2015). IF . PubMed: 26431344
    • Wasserman DI  et al. Cholinergic control of morphine-induced locomotion in rostromedial tegmental nucleus versus ventral tegmental area sites. Eur J Neurosci 38:2774-85 (2013). IHC-FoFr ; Mouse . PubMed: 23773170

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-3 of 3 Abreviews or Q&A

    Question

    Inquiry: Ich hätte eine Frage zu zwei Antikörpern, zum einen gg. den Acetylcholin-Rezeptor M5 (ab41171) und gg. die nicotinerge ACh-Rezeptor Untereinheit alpha7 (ab23832): Gibt es die Möglichkeit eine kleinere Menge zu erhalten bzw. ordern um den Antikörper testen zu können? MfG,

    Read More
    Answer

    Vielen Dank für Ihre Anfrage.

    Leider bieten wir generell keine kostenlosen Proben zu Testzwecken an. Hier bei Abcam verfahren wir so, dass wir einen kostenlosen Ersatz oder eine Gutschrift verschicken, falls ein Antikörper nicht so funktioniert wie auf unserem Datenblatt beschrieben. Falls der Antikörper in einer bisher nicht getesteten Anwendung oder Spezies verwendet wird, können wir leider keinen Ersatz anbieten, wir möchten Sie jedoch einladen unser Abreview-System zu verwenden.

    https://www.abcam.com/abtrial

    Das Abreview-System erlaubt es unseren Kunden, Rezensionen unserer Produkte zu veröffendlichen und dafür Punkte zu erhalten. Diese “Abpoints” können gegen Rabatte beim Kauf weiterer Produkte oder Geschenkgutscheine wie z.B. Amazon.com-Gutscheine eingetauscht werden. Sollten Sie sich dazu entscheiden, dieses Produkt zu kaufen, teilen Sie uns bitte Ihre Ergebnisse mittels eines „Abreview“ mit. Sie können die Rezension durch einen Link direkt auf unserem Produktdatenblatt abschicken.


    Ich hoffe, dies hilft Ihnen weiter. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

    Read More

    Question

    Inquiry: Hi, I am interested in the above antibody and was wondering if you have any information about its use for IHC in PFA-perfused rodent brain? It would be helpful to you have images of sections stained with this antibody before deciding on a purchase. Thank you very much

    Read More
    Answer

    Thank you for your previous email, and I sincerely apologize for the extended delay getting back to you.

    I have not been able to obtain any images of this antibody using in tissue staining, and I am sorry that we don't have these available.

    As I mentioned before, the antibody has only been tested in Western blot, ELISA, and IHC on standard PFA-fixed tissue.We do fully guarantee the antibody to work in these tested applications, however if you would like to try the antibody in IHC of PFA-perfused tissue (IHC-FoFr), we do have a testing discount program that you might be interested in. In this program, you would purchase ab41171 and test it in IHC-FoFr, then after submitting an Abreview with your data and protocol, we will give you one free primary antibody on a future order. I'd be happy to send more information if you are interested in the program.

    Please let me know if you have any additional questions or if there is anything else that we can do for you.

    Read More

    Question

    Inquiry: Hi, I am interested in the above antibody and was wondering if you have any information about its use for IHC in PFA-perfused rodent brain? It would be helpful to you have images of sections stained with this antibody before deciding on a purchase. Thank you very much,

    Read More
    Answer

    Thank you for contacting us with your question.

    I've looked through our records andto our knowledge, the antibody has only been tested inWestern blot, ELISA, and IHC on standard PFA-fixed tissue. I will get in touch with the lab and request images from the antibody testing in these applications.

    We do fully guarantee the antibody to work in these tested applications, however if you would like to try the antibody in IHC of PFA-perfused tissue (IHC-FoFr), we do have a testing discount program that you might be interested in. In this program, you would purchase ab41171 and test it in IHC-FoFr, then after submitting an Abreview with your data and protocol, we will give you one free primary antibody on a future order. I'd be happy to send more information if you are interested in the program.

    I'll be back in touch once I hear from the lab. Please let me know if you have any questions or if there is anything else we can do for you in the meantime.

    Read More

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