Anti-Muscarinic Acetylcholine Receptor M3/CHRM3 抗体 (ab87199)
Key features and details
- Rabbit polyclonal to Muscarinic Acetylcholine Receptor M3/CHRM3
- Suitable for: WB, IHC-P
- Reacts with: Rat
- Isotype: IgG
製品の概要
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製品名
Anti-Muscarinic Acetylcholine Receptor M3/CHRM3 antibody
Muscarinic Acetylcholine Receptor M3/CHRM3 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Muscarinic Acetylcholine Receptor M3/CHRM3 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Rat
交差が予測される動物種: Mouse, Sheep, Rabbit, Horse, Cow, Pig, Chinese hamster
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免疫原
Synthetic peptide corresponding to Rat Muscarinic Acetylcholine Receptor M3/CHRM3 aa 550 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab139964) -
ポジティブ･コントロール
- This antibody gave a positive signal in the following Rat tissue lysates: Brain; Forebrain; Substantia Nigra; Heart.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, 98.98% PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab87199の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 85 kDa (predicted molecular weight: 66 kDa).
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| IHC-P |
Use a concentration of 5 µg/ml.
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| 特記事項 |
|---|
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 85 kDa (predicted molecular weight: 66 kDa). |
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IHC-P
Use a concentration of 5 µg/ml. |
ターゲット情報
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機能
The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover. -
配列類似性
Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM3 sub-subfamily. -
細胞内局在
Cell membrane. Cell junction > synapse > postsynaptic cell membrane. Basolateral cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 281685 Cow
- Entrez Gene: 12671 Mouse
- Entrez Gene: 100144478 Pig
- Entrez Gene: 24260 Rat
- SwissProt: P41984 Cow
- SwissProt: Q9ERZ3 Mouse
- SwissProt: P11483 Pig
- SwissProt: P08483 Rat
see all -
別名
- AChR antibody
- ACM3_HUMAN antibody
- cholinergic receptor muscarinic 3 antibody
see all
画像
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Western blot - Anti-Muscarinic Acetylcholine Receptor M3/CHRM3 antibody (ab87199)All lanes : Anti-Muscarinic Acetylcholine Receptor M3/CHRM3 antibody (ab87199) at 1 µg/ml
Lane 1 : Brain (Rat) Tissue Lysate
Lane 2 : Rat Forebrain Rat Tissue Lysate
Lane 3 : Rat Substantia Nigra Tissue Lysate
Lane 4 : Heart (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 66 kDa
Observed band size: 85 kDa why is the actual band size different from the predicted?
Additional bands at: 16 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutesMuscarinic Acetylcholine Receptor M3/CHRM3 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Muscarinic Acetylcholine Receptor M3/CHRM3 antibody (ab87199)IHC image of Muscarinic Acetylcholine Receptor M3/CHRM3 staining in Rat normal brain (Sagittal) formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab87199, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (9)
ab87199 は 9 報の論文で使用されています。
- DeSpain K et al. Carotid smooth muscle contractility changes after severe burn. Sci Rep 11:18094 (2021). PubMed: 34508162
- Chiang BJ et al. Adipose-Derived Stem Cells and Their Derived Microvesicles Ameliorate Detrusor Overactivity Secondary to Bilateral Partial Iliac Arterial Occlusion-Induced Bladder Ischemia. Int J Mol Sci 22:N/A (2021). PubMed: 34210091
- Wang Y et al. Shugan Decoction Alleviates Colonic Dysmotility in Female SERT-Knockout Rats by Decreasing M3 Receptor Expression. Front Pharmacol 11:01082 (2020). PubMed: 33013355
- Tarafder S et al. A Combination of Oxo-M and 4-PPBP as a potential regenerative therapeutics for tendon injury. Theranostics 9:4241-4254 (2019). PubMed: 31281545
- Wu KC et al. I-Tiao-Gung extract through its active component daidzin improves cyclophosphamide-induced bladder dysfunction in rat model. Neurourol Urodyn 37:2560-2570 (2018). PubMed: 30252154
- Kashyap MP et al. BDNF overexpression in the bladder induces neuronal changes to mediate bladder overactivity. Am J Physiol Renal Physiol 315:F45-F56 (2018). PubMed: 29092846
- Wilson C et al. Clusters of specialized detector cells provide sensitive and high fidelity receptor signaling in the intact endothelium. FASEB J 30:2000-13 (2016). ICC . PubMed: 26873937
- Nishijima H et al. Denervation of nasal mucosa induced by posterior nasal neurectomy suppresses nasal secretion, not hypersensitivity, in an allergic rhinitis rat model. Lab Invest 96:981-93 (2016). PubMed: 27322954
- Eckhard A et al. Regulation of the perilymphatic-endolymphatic water shunt in the cochlea by membrane translocation of aquaporin-5. Pflugers Arch 467:2571-88 (2015). IF . PubMed: 26208470