Anti-Muscarinic Acetylcholine Receptor 2/CM2 抗体 [31-1D1] (ab2805)
![Western blot - Anti-Muscarinic Acetylcholine Receptor 2/CM2 antibody [31-1D1] (ab2805)](https://www.abcam.co.jp/ps/products/2/ab2805/Images/ab2805-6452-anti-muscarinic-acetylcholine-receptor-2-antibody-31-1d1-western-blot.jpg "Western blot - Anti-Muscarinic Acetylcholine Receptor 2/CM2 antibody [31-1D1] (ab2805)")
Key features and details
- Mouse monoclonal [31-1D1] to Muscarinic Acetylcholine Receptor 2/CM2
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
製品の概要
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製品名
Anti-Muscarinic Acetylcholine Receptor 2/CM2 antibody [31-1D1]
Muscarinic Acetylcholine Receptor 2/CM2 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [31-1D1] to Muscarinic Acetylcholine Receptor 2/CM2 -
由来種
Mouse -
特異性
This antibody is specific for the m2 mAChR subtype. -
アプリケーション
適用あり: WB, ICC/IF, IHC-Pmore details -
種交差性
交差種: Mouse, Rat, Human
非交差種: Chicken -
免疫原
Full length native protein (purified) corresponding to Pig Muscarinic Acetylcholine Receptor 2/CM2. Purified from Pig Heart.
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ポジティブ･コントロール
- WB: Human brain and spinal cord tissue lysate. Mouse brain tissue lysate. IHC-P: Human kidney tissue. ICC/IF: PC12 cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide
Constituent: 0.1% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
31-1D1 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab2805の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (5) |
1/1000. Detects a band of approximately 64 kDa (predicted molecular weight: 52 kDa).
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| ICC/IF |
Use at an assay dependent concentration.
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| IHC-P |
1/20.
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| 特記事項 |
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WB
1/1000. Detects a band of approximately 64 kDa (predicted molecular weight: 52 kDa). |
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ICC/IF
Use at an assay dependent concentration. |
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IHC-P
1/20. |
ターゲット情報
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機能
The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is adenylate cyclase inhibition. -
関連疾患
Genetic variations in CHRM2 can influence susceptibility to major depressive disorder (MDD) [MIM:608516]. MDD is one of the most common psychiatric disorders. MDD is a complex trait characterized by one or more major depressive episodes without a history of manic, mixed, or hypomanic episodes. A major depressive episode is characterized by at least 2 weeks during which there is a new onset or clear worsening of either depressed mood or loss of interest or pleasure in nearly all activities. Four additional symptoms must also be present including changes in appetite, weight, sleep, and psychomotor activity; decreased energy; feelings of worthlessness or guilt; difficulty thinking, concentrating, or making decisions; or recurrent thoughts of death or suicidal ideation, plans, or attempts. The episode must be accompanied by distress or impairment in social, occupational, or other important areas of functioning. -
配列類似性
Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM2 sub-subfamily. -
細胞内局在
Cell membrane. Cell junction > synapse > postsynaptic cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 1129 Human
- Entrez Gene: 243764 Mouse
- Entrez Gene: 81645 Rat
- Omim: 118493 Human
- SwissProt: P08172 Human
- SwissProt: Q9ERZ4 Mouse
- SwissProt: P10980 Rat
- Unigene: 535891 Human
see all -
別名
- 7TM receptor antibody
- Acetylcholine receptor, muscarinic, 2 antibody
- AChR antibody
see all
画像
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Western blot - Anti-Muscarinic Acetylcholine Receptor 2/CM2 antibody [31-1D1] (ab2805)All lanes : Anti-Muscarinic Acetylcholine Receptor 2/CM2 antibody [31-1D1] (ab2805) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Human spinal cord tissue lysate - total protein (ab29188)
Lane 3 : Brain (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 52 kDa
Observed band size: 64 kDa why is the actual band size different from the predicted?
Additional bands at: 22 kDa, 40 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 secondsMuscarinic Acetylcholine Receptor 2/CM2 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Muscarinic Acetylcholine Receptor 2/CM2 antibody [31-1D1] (ab2805)](https://www.abcam.co.jp/ps/products/2/ab2805/Images/ab2805-200555-anti-muscarinic-acetylcholine-receptor-2-antibody-31-1d1-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Muscarinic Acetylcholine Receptor 2/CM2 antibody [31-1D1] (ab2805)Immunohistochemistry was performed on normal biopsies of deparaffinized human kidney tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer and microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a Mouse monoclonal antibody recognizing Muscarinic Acetylcholine Receptor 2/CM2 (ab2805) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
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![Immunocytochemistry/ Immunofluorescence - Anti-Muscarinic Acetylcholine Receptor 2/CM2 antibody [31-1D1] (ab2805)](https://www.abcam.co.jp/ps/products/2/ab2805/Images/ab2805-6453-anti-muscarinic-acetylcholine-receptor-2-antibody-31-1d1-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Muscarinic Acetylcholine Receptor 2/CM2 antibody [31-1D1] (ab2805)ICC/IF image of ab2805 stained PC12 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2805, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (13)
ab2805 は 13 報の論文で使用されています。
- Ma M et al. Aerobic exercise ameliorates cardiac hypertrophy by regulating mitochondrial quality control and endoplasmic reticulum stress through M2 AChR. J Cell Physiol 236:6581-6596 (2021). PubMed: 33615478
- Ma G et al. Association of Autoantibodies against M2-Muscarinic Acetylcholine Receptor with Atrial Fibrosis in Atrial Fibrillation Patients. Cardiol Res Pract 2019:8271871 (2019). PubMed: 30863630
- Leonhäuser D et al. Expression of components of the urothelial cholinergic system in bladder and cultivated primary urothelial cells of the pig. BMC Urol 19:62 (2019). PubMed: 31288793
- Tarafder S et al. A Combination of Oxo-M and 4-PPBP as a potential regenerative therapeutics for tendon injury. Theranostics 9:4241-4254 (2019). PubMed: 31281545
- Deng Y et al. Angiotensin-Converting Enzyme 2 in the Rostral Ventrolateral Medulla Regulates Cholinergic Signaling and Cardiovascular and Sympathetic Responses in Hypertensive Rats. Neurosci Bull 35:67-78 (2019). PubMed: 30318562
