Anti-Muscarinic Acetylcholine Receptor 1/CHRM1 抗体 (ab77098)
Key features and details
- Goat polyclonal to Muscarinic Acetylcholine Receptor 1/CHRM1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Muscarinic Acetylcholine Receptor 1/CHRM1 antibody -
製品の詳細
Goat polyclonal to Muscarinic Acetylcholine Receptor 1/CHRM1 -
由来種
Goat -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Dog, Pig, Rhesus monkey, Orangutan
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免疫原
Synthetic peptide corresponding to Human Muscarinic Acetylcholine Receptor 1/CHRM1 aa 438-453 (C terminal).
Sequence:C-DKRRWRKIPKRPGSVH
Database link: NP_000729.2 -
ポジティブ･コントロール
- Human brain lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab77098 is purified from goat serum by ammonium sulphate precipitation, followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab77098の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 51 kDa.
1 hour primary incubation is recommended for this product. |
| 特記事項 |
|---|
|
WB
Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 51 kDa. 1 hour primary incubation is recommended for this product. |
ターゲット情報
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関連性
The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 1 is involved in mediation of vagally-induced bronchoconstriction and in the acid secretion of the gastrointestinal tract. Muscarinic receptors are so called as they are more sensitive to the effects of the alkaloid muscarine, rather than nicotine. -
細胞内局在
Cell Membrane; multi-pass membrane protein -
参照データベース
- Entrez Gene: 1128 Human
- Entrez Gene: 12669 Mouse
- Entrez Gene: 397099 Pig
- Entrez Gene: 25229 Rat
- Omim: 118510 Human
- SwissProt: P11229 Human
- SwissProt: P12657 Mouse
- SwissProt: Q5R949 Orangutan
see all -
別名
- AChR antibody
- Cholinergic receptor muscarinic 1 antibody
- CHRM1 antibody
see all
画像
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Western blot - Anti-Muscarinic Acetylcholine Receptor 1/CHRM1 antibody (ab77098)Anti-Muscarinic Acetylcholine Receptor 1/CHRM1 antibody (ab77098) at 0.300000011920929 µg/ml + human brain lysate (in RIPA buffer) at 35 µg
Predicted band size: 51 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab77098 は 4 報の論文で使用されています。
- Saha S et al. IMPA1 dependent regulation of phosphatidylinositol 4,5-bisphosphate and calcium signalling by lithium. Life Sci Alliance 7:N/A (2024). PubMed: 38056909
- Tsolias A & Medalla M Muscarinic Acetylcholine Receptor Localization on Distinct Excitatory and Inhibitory Neurons Within the ACC and LPFC of the Rhesus Monkey. Front Neural Circuits 15:795325 (2021). PubMed: 35087381
- Dankis M et al. Novel Insights Into Muscarinic and Purinergic Responses in Primary Cultures of Rat Lacrimal Gland Myoepithelial Cells. Invest Ophthalmol Vis Sci 62:19 (2021). PubMed: 34546325
- Winder M et al. Autonomic Receptor-mediated Regulation of Production and Release of Nitric Oxide in Normal and Malignant Human Urothelial Cells. Basic Clin Pharmacol Toxicol 121:257-265 (2017). PubMed: 28437032