Anti-MURF2 抗体 (ab4387)
Key features and details
- Goat polyclonal to MURF2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-MURF2 antibody -
製品の詳細
Goat polyclonal to MURF2 -
由来種
Goat -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Rat, Dog, Pig
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免疫原
Synthetic peptide:
HIFSFSWLNSLNE
, corresponding to C terminal amino acids 536-546 of Human MURF2. -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab4387の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 60 kDa.
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| IHC-P |
Use a concentration of 10 µg/ml.
|
| 特記事項 |
|---|
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 60 kDa. |
|
IHC-P
Use a concentration of 10 µg/ml. |
ターゲット情報
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機能
May regulate gene expression and protein turnover in muscle cells. -
組織特異性
Highly expressed in muscle. Low-level expression in liver. -
配列類似性
Contains 1 B box-type zinc finger.
Contains 1 COS domain.
Contains 1 RING-type zinc finger. -
細胞内局在
Cytoplasm. Nucleus. Nuclear under atrophic conditions and upon mechanical signals. Localizes to the sarcomeric M-band in cardiomyocytes. Colocalizes in part with microtubules. - Information by UniProt
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参照データベース
- Entrez Gene: 84675 Human
- Entrez Gene: 365751 Rat
- Omim: 606469 Human
- SwissProt: Q9BYV6 Human
- SwissProt: Q5PQN5 Rat
- Unigene: 85524 Human
- Unigene: 76142 Rat
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別名
- MURF 2 antibody
- MURF-2 antibody
- MuRF2 antibody
see all
画像
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Western blot - Anti-MURF2 antibody (ab4387)Anti-MURF2 antibody (ab4387) at 0.1 µg/ml + Human heart lysate (in RIPA buffer) at 35 µg
Predicted band size: 60 kDa
データシートおよび資料
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Datasheet download
参考文献 (8)
ab4387 は 8 報の論文で使用されています。
- Fahrner A et al. microRNA-501 controls myogenin+/CD74+ myogenic progenitor cells during muscle regeneration. Mol Metab 71:101704 (2023). PubMed: 36907509
- Fan Y et al. Lysine 222 in PPAR γ1 functions as the key site of MuRF2-mediated ubiquitination modification. Sci Rep 13:1999 (2023). PubMed: 36737649
- Müller E et al. E3-ligase knock down revealed differential titin degradation by autopagy and the ubiquitin proteasome system. Sci Rep 11:21134 (2021). PubMed: 34702928
- He J et al. MuRF2 regulates PPAR?1 activity to protect against diabetic cardiomyopathy and enhance weight gain induced by a high fat diet. Cardiovasc Diabetol 14:97 (2015). WB . PubMed: 26242235
- Cullup T et al. Recessive mutations in EPG5 cause Vici syndrome, a multisystem disorder with defective autophagy. Nat Genet 45:83-7 (2013). PubMed: 23222957
- Perera S et al. Developmental regulation of MURF E3 ubiquitin ligases in skeletal muscle. J Muscle Res Cell Motil 33:107-22 (2012). PubMed: 22426552
- Perera S et al. Developmental regulation of MURF ubiquitin ligases and autophagy proteins nbr1, p62/SQSTM1 and LC3 during cardiac myofibril assembly and turnover. Dev Biol 351:46-61 (2011). WB ; Mouse . PubMed: 21185285
- Willis MS et al. Muscle ring finger 1, but not muscle ring finger 2, regulates cardiac hypertrophy in vivo. Circ Res 100:456-9 (2007). WB ; Mouse . PubMed: 17272810
