AB232364

Anti-MURF1 + MURF3 + MURF2 抗体 [EPR6431(2)] - BSA and Azide free

Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal MURF2 antibody. Carrier free. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 1 publication.

別名を表示する

MURF2, RNF29, TRIM55, Tripartite motif-containing protein 55, Muscle-specific RING finger protein 2, RING finger protein 29, MuRF-2, MuRF2, IRF, MURF1, RNF28, SMRZ, TRIM63, E3 ubiquitin-protein ligase TRIM63, Iris RING finger protein, Muscle-specific RING finger protein 1, RING finger protein 28, RING-type E3 ubiquitin transferase TRIM63, Striated muscle RING zinc finger protein, Tripartite motif-containing protein 63, MuRF-1, MuRF1, MURF, MURF3, RNF30, TRIM54, Tripartite motif-containing protein 54, Muscle-specific RING finger protein, Muscle-specific RING finger protein 3, RING finger protein 30, MuRF, MuRF-3, MuRF3

6 Images

Supplier Data

Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] - BSA and Azide free (AB232364)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab172479).

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (<a href='/products/primary-antibodies/murf1-murf3-murf2-antibody-epr64312-ab172479'>ab172479</a>) at 1/500 dilution

All lanes:

His-Tagged human MURF2 (full length) recombinant protein at 1 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 75 kDa

false

Exposure time: 150s

Supplier Data

Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] - BSA and Azide free (AB232364)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab172479).

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (<a href='/products/primary-antibodies/murf1-murf3-murf2-antibody-epr64312-ab172479'>ab172479</a>) at 1/1000 dilution

All lanes:

His-Tagged human MURF3 (full length) recombinant protein at 1 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 45 kDa

false

Exposure time: 180s

Supplier Data

Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] - BSA and Azide free (AB232364)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab172479).

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (<a href='/products/primary-antibodies/murf1-murf3-murf2-antibody-epr64312-ab172479'>ab172479</a>) at 1/1000 dilution

All lanes:

His-Tagged human MURF1 (aa 1 to 353) recombinant protein at 1 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 60 kDa

false

Exposure time: 30s

Supplier Data

Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] - BSA and Azide free (AB232364)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab172479).

All lanes:

Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (<a href='/products/primary-antibodies/murf1-murf3-murf2-antibody-epr64312-ab172479'>ab172479</a>) at 1/1000 dilution

Lane 1:

Rat muscle tissue lysate at 10 µg

Lane 2:

Mouse muscle tissue lysate at 10 µg

Lane 3:

Human skeletal muscle tissue lysate at 10 µg

false

Lab

Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] - BSA and Azide free (AB232364)

Blocking and diluting buffer : 5% NFDM/TBST

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab172479).

All lanes:

Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (<a href='/products/primary-antibodies/murf1-murf3-murf2-antibody-epr64312-ab172479'>ab172479</a>) at 1/1000 dilution

Lane 1:

Human skeletal muscle lysate at 20 µg

Lane 2:

Mouse muscle lysate at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

Observed band size: 40 kDa

false

Lab

Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] - BSA and Azide free (AB232364)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab172479).

Blocking and diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (<a href='/products/primary-antibodies/murf1-murf3-murf2-antibody-epr64312-ab172479'>ab172479</a>) at 1/5000 dilution

All lanes:

Mouse muscle lysate at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

Observed band size: 40 kDa

false

Exposure time: 150s

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR6431(2)

アイソタイプ

IgG

キャリアフリー

Yes

交差種

Mouse, Rat, Human

アプリケーション

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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製品の詳細

ab232364 is the carrier-free version of ab172479.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Constituents: PBS

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

Do Not Freeze

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

E3 ubiquitin ligase that plays an important role in regulating cardiac development and contractility, muscle growth, metabolism, and fiber-type differentiation. Acts as a critical factor that regulates cardiomyocyte size during development in concert with TRIM63 by regulating E2F1-mediated gene expression (By similarity). Plays a role in apoptosis induction in cardiomyocytes by promoting ubiquitination of the DUSP1 phosphatase. Promotes non-canonical NF-kappa-B signaling and B-cell-mediated immune responses by mediating NFKB2 'Lys-48'-linked ubiquitination and processing. In turn, NFKB2 is further processed by valosin-containing protein/VCP, an ATPase that mediates ubiquitin-dependent protein degradation by the proteasome. May play a role in preventing macrophages from producing inflammatory factors and migrating by downregulating the level of nuclear NF-kappa-B subunit RELA. Also modifies PPARG via polyubiquitination and accelerates PPARG proteasomal degradation to inhibit its activity (PubMed : 36737649).

See full target information TRIM55

追加のターゲット

,TRIM63

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 23: PubMed36499021

2022

A New Role of NAP1L1 in Megakaryocytes and Human Platelets.

Applications

Unspecified application

Species

Unspecified reactive species

Martin Freitag,Hansjörg Schwertz

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MURF1 + MURF3 + MURF2 抗体 [EPR6431(2)] - BSA and Azide free

Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] - BSA and Azide free

Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] - BSA and Azide free (AB232364)

Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] - BSA and Azide free (AB232364)

Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] - BSA and Azide free (AB232364)

Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] - BSA and Azide free (AB232364)

Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] - BSA and Azide free (AB232364)

Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] - BSA and Azide free (AB232364)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

保管に関する情報

製品プロトコール

ターゲットの情報

追加のターゲット

文献 (1)

Abcam product promise