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リコンビナントRabMAb

Anti-MURF1 + MURF3 + MURF2 抗体 [EPR6431(2)] (ab172479)

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Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479)
  • Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479)
  • Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479)
  • Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479)
  • Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479)
  • Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479)
  • Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6431(2)] to MURF1 + MURF3 + MURF2
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)]

  • 製品の詳細

    Rabbit monoclonal [EPR6431(2)] to MURF1 + MURF3 + MURF2

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WBmore details

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide within Human MURF1 aa 1-100. The exact sequence is proprietary.
    Database link: Q969Q1

  • ポジティブ･コントロール

    • Rat muscle, Mouse muscle and Human skeletal muscle tissue lysate.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab172479の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000 - 1/5000. Predicted molecular weight: 40 kDa.
特記事項
WB
1/1000 - 1/5000. Predicted molecular weight: 40 kDa.

ターゲット情報

画像

  • Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479)
    Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479)
    All lanes : Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479) at 1/1000 dilution

    Lane 1 : Human skeletal muscle lysate
    Lane 2 : Mouse muscle lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

    Predicted band size: 40 kDa
    Observed band size: 40 kDa



    Blocking and diluting buffer: 5% NFDM/TBST

  • Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479)
    Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479)
    All lanes : Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479) at 1/1000 dilution

    Lane 1 : Rat muscle tissue lysate
    Lane 2 : Mouse muscle tissue lysate
    Lane 3 : Human skeletal muscle tissue lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 40 kDa

  • Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479)
    Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479)
    Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479) at 1/1000 dilution + His-Tagged human MURF1 (aa 1 to 353) recombinant protein at 1 µg with 5% NFDM/TBST

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 40 kDa
    Observed band size: 60 kDa why is the actual band size different from the predicted?


    Exposure time: 30 seconds
  • Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479)
    Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479)
    Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479) at 1/1000 dilution + His-Tagged human MURF3 (full length) recombinant protein at 1 µg with 5% NFDM/TBST

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 40 kDa
    Observed band size: 45 kDa why is the actual band size different from the predicted?


    Exposure time: 180 seconds
  • Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479)
    Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479)
    Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479) at 1/500 dilution + His-Tagged human MURF2 (full length) recombinant protein at 1 µg with 5% NFDM/TBST

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 40 kDa
    Observed band size: 75 kDa why is the actual band size different from the predicted?


    Exposure time: 150 seconds
  • Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479)
    Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479)
    Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479) at 1/5000 dilution + Mouse muscle lysate at 20 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

    Predicted band size: 40 kDa
    Observed band size: 40 kDa


    Exposure time: 150 seconds


    Blocking and diluting buffer: 5% NFDM/TBST

  • Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479)
    Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479)

参考文献 (53)

ab172479 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab172479 は 53 報の論文で使用されています。

  • Miyazaki M  et al. Supplementing cultured human myotubes with hibernating bear serum results in increased protein content by modulating Akt/FOXO3a signaling. PLoS One 17:e0263085 (2022). PubMed: 35077510
  • Ma X  et al. Endoplasmic Reticulum Stress Is Involved in Muscular Pathogenesis in Idiopathic Inflammatory Myopathies. Front Cell Dev Biol 10:791986 (2022). PubMed: 35237595
  • Duan J  et al. Comparison of the effects of different calorie amounts of enteral nutrition in hypercatabolism associated with ghrelin-POMC in endotoxemic rats. Nutr Metab (Lond) 19:28 (2022). PubMed: 35428321
  • Li S  et al. Endoplasmic Reticulum Stress Contributes to Ventilator-Induced Diaphragm Atrophy and Weakness in Rats. Front Physiol 13:897559 (2022). PubMed: 35832486
  • Testa MTJ  et al. Resistance Training Attenuates Activation of STAT3 and Muscle Atrophy in Tumor-Bearing Mice. Front Oncol 12:880787 (2022). PubMed: 35847939
View all Publications for this product

レビューと Q&A

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レビューを送る 質問を送る

Western blot abreview for Anti-MURF1 antibody [EPR6431(2)]

 Inconclusive
Abreviews
abreview image
Application
Western blot
Sample
Rat Tissue lysate - other (muscle (cytosolic protein))
Gel Running Conditions
Reduced Denaturing (9)
Loading amount
50 µg
Specification
muscle (cytosolic protein)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Baptiste Jude

Verified customer

投稿 Oct 13 2016

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