Anti-Munc18c 抗体 [EPR12447] (ab175238)
![Western blot - Anti-Munc18c antibody [EPR12447] (ab175238)](https://www.abcam.co.jp/ps/products/175/ab175238/Images/ab175238-201748-anti-munc18c-antibody-epr12447-western-blot.jpg "Western blot - Anti-Munc18c antibody [EPR12447] (ab175238)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12447] to Munc18c
- Suitable for: WB, ICC/IF, IP
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Munc18c antibody [EPR12447]
Munc18c 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR12447] to Munc18c -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IF, IPmore details
適用なし: Flow Cyt or IHC-P -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- Human fetal kidney, Human spleen and K562 lysates; K562 cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EPR12447 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab175238の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/10000 - 1/50000. Predicted molecular weight: 68 kDa.
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| ICC/IF |
1/100 - 1/250.
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| IP |
1/10 - 1/100.
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| 特記事項 |
|---|
|
WB
1/10000 - 1/50000. Predicted molecular weight: 68 kDa. |
|
ICC/IF
1/100 - 1/250. |
|
IP
1/10 - 1/100. |
ターゲット情報
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機能
Together with STX4 and VAMP2, may play a role in insulin-dependent movement of GLUT4 and in docking/fusion of intracellular GLUT4-containing vesicles with the cell surface in adipocytes. -
組織特異性
Megakaryocytes and platelets. -
配列類似性
Belongs to the STXBP/unc-18/SEC1 family. -
翻訳後修飾
Phosphorylated by PKC in platelets in response to thrombin stimulation; phosphorylation inhibits binding to STX4. -
細胞内局在
Cytoplasm > cytosol. Cell membrane. In platelets, predominantly cytosolic. Low amounts membrane-associated. - Information by UniProt
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参照データベース
- Entrez Gene: 6814 Human
- Omim: 608339 Human
- SwissProt: O00186 Human
- Unigene: 530436 Human
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別名
- Munc18 3 antibody
- Munc18 c antibody
- Platelet Sec1 protein antibody
see all
画像
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Western blot - Anti-Munc18c antibody [EPR12447] (ab175238)All lanes : Anti-Munc18c antibody [EPR12447] (ab175238) at 1/10000 dilution
Lane 1 : Human fetal kidney tissue lysate
Lane 2 : Human spleen lysate
Lane 3 : K562 lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 68 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-Munc18c antibody [EPR12447] (ab175238)](https://www.abcam.co.jp/ps/products/175/ab175238/Images/ab175238-201746-anti-munc18c-antibody-epr12447-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Munc18c antibody [EPR12447] (ab175238)Immunofluorescent analysis of K562 cells labeling Munc18c with ab175238 at 1/100 (red) and DAPI staining (blue).
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![Immunoprecipitation - Anti-Munc18c antibody [EPR12447] (ab175238)](https://www.abcam.co.jp/ps/products/175/ab175238/Images/ab175238-201747-anti-munc18c-antibody-epr12447-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Munc18c antibody [EPR12447] (ab175238)Immunoprecipitation of Munc18c from Human fetal kidney lysate using ab175238 at 1/10
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![Anti-Munc18c antibody [EPR12447] (ab175238)](https://www.abcam.co.jp/ps/products/175/ab175238/Images/ab175238-4-benefits-of-recombinant-antibodies.png)
Anti-Munc18c antibody [EPR12447] (ab175238)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab175238 は 2 報の論文で使用されています。
- Brasher MI et al. Syntaxin4-Munc18c Interaction Promotes Breast Tumor Invasion and Metastasis by Regulating MT1-MMP Trafficking. Mol Cancer Res 20:434-445 (2022). PubMed: 34876482
- Brasher MI et al. Interaction of Munc18c and syntaxin4 facilitates invadopodium formation and extracellular matrix invasion of tumor cells. J Biol Chem 292:16199-16210 (2017). PubMed: 28798239